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AAV-RO1.7-Lrat

Plasmid

#206345

Purpose

AAV plasmid expressing LRAT in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

Insert

Lrat (Lrat Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

1.7kb human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN-CD4

Plasmid

#175942

Purpose

knockout mouse CD4

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

CD4 gRNA (Cd4 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Trib3

Plasmid

#131157

Purpose

Mammalian expression of flag-tagged mouse TRIB3

Depositor

Karen Keeshan, Warren Pear

Article

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib3 (Trib3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-Rosmarinus

Plasmid

#183462

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

Rosmarinus

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Nxph4-HA

Plasmid

#128102

Purpose

Express HA tagged Nxph4

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

Plasmid

#112829

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

Insert

LL5BIP (2410002F23Rik Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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mChT pcDNA3

Plasmid

#15765

Depositor

Randy Blakely

Article

Apparsundaram et al Biochem Soc Trans. 2001 Nov . 29(Pt 6):7

Insert

hemicholinium-3-sensitive choline transporter (Slc5a7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
iDuet101a-mCHF1

Plasmid

#55611

Purpose

Lentivirus containing mouse Hey2 coding region

Depositor

Michael Chin

Article

Hartman et al PLoS One. 2014 Mar 25;9(3):e93033. doi:

Insert

Hey2 (Hey2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pB-puro-V5-Trpt1

Plasmid

#126607

Purpose

Expresses N-terminal V5-tagged mouse Trpt1

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

tRNA 2' phosphotransferase 1 (Trpt1 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/42]

Plasmid

#190488

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/42.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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CBP-HAT-pET

Plasmid

#21091

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

Tags

His and T7 tag

Expression

Bacterial

Mutation

HAT domain aa 1171-1621

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
Fgf4enhLV (FpG12)

Plasmid

#69444

Purpose

positive control plasmid for lentiviral reporter expression in ESCs

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Inserts

minimal FGF4 promoter
EGFP
Ubiquitin promoter
Hygromycin resistance
FGF4 enhancer (Fgf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R]

Plasmid

#149455

Purpose

Mammalian Expression Plasmid of anti-MMP9 matrix metalloproteinase-9 precursor (Rat). Derived from hybridoma L51/82.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MMP9 matrix metalloproteinase-9 precursor (Rattus norvegicus) recombinant mouse monoclonal antibody (Mmp9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV KSR1 IRES GFP

Plasmid

#25973

Depositor

Rob Lewis

Article

Kortum et al Mol Cell Biol. 2004 May . 24(10):4407-16

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pBICD2-TUG

Plasmid

#52873

Purpose

retroviral expression of TUG

Depositor

Jonathan Bogan

Article

Bogan et al Nature. 2003 Oct 16;425(6959):727-33.

Insert

TUG (Aspscr1 Mouse)

Use

Retroviral

Tags

IRES-CD2 (mouse)

Expression

Mammalian

Mutation

Promoter

retrovirus LTR

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pMRX-IP/Venus-mDFCP1

Plasmid

#58742

Purpose

Expresses Venus-mDFCP1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

Venus

Expression

Mutation

Promoter

Available since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
attB-mCh-Dnmt3b1-Poly(A)-PuroR

Plasmid

#65556

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 1

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-Nedd4-N / 2524 JM16

Plasmid

#13312

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

aa1-52 and aa422-777 deleted

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-SM

Plasmid

#136578

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-E2A-cMyc (Sox2, Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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HA-RNF126 C231A

Plasmid

#138648

Purpose

Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)

Depositor

Jane McGlade

Article

Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

Insert

RNF126 (RNF126 Mouse)

Use

Tags

HA-HA

Expression

Mammalian

Mutation

C231A

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)

Plasmid

#156163

Purpose

Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated THBS1 (amino acid residues 1-954)

Promoter

CMV

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1mLCAD_FLAG

Plasmid

#24484

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

Plasmid

#193348

Purpose

tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458_miR-290-295KO-gRNA1

Plasmid

#172709

Purpose

Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

miR-290-295

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_miR-290-295KO-gRNA2

Plasmid

#172710

Purpose

Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

miR-290-295

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Unc5c.a-Fc-His

Plasmid

#72180

Purpose

Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.a (Unc5c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SEP-Nanoluc-cyOFP1

Plasmid

#141082

Purpose

prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc-cyOFP1

Use

Lentiviral

Tags

Expression

Mutation

Deletion of amino acids DISGG from positions 673 …

Promoter

EF1α

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 SV8

Plasmid

#184011

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Wild type cDNA

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
3265 pBS Mcl-1

Plasmid

#8763

Depositor

Stanley Korsmeyer

Article

Rinkenberger et al Genes Dev 2000 Jan 1;14(1):23-7.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
mEmerald-MCM6-N-22

Plasmid

#54172

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM6

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-H1-10

Plasmid

#56161

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H1 (H1f0 Mouse)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCB410 (His-Ub-Ate1-4 1A7B)

Plasmid

#104339

Purpose

Express His Tag Ate1-4 1A7B

Depositor

Alexander Varshavsky

Insert

ATE1 (Ate1 Mouse)

Use

Tags

6xHis-Ub

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac flag Bmi1

Plasmid

#1967

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Bmi1 (Bmi1 Mouse)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
mEos2-Talin-H-18

Plasmid

#57422

Purpose

Localization: Talin Head Domain, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin H

Use

Tags

mEos2

Expression

Mammalian

Mutation

Talin Head Domain

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAav-MCS-PQS2-3xHA

Plasmid

#84917

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

LOX-PGK-NEO-LOX

Use

AAV

Tags

PQS2 3xHA

Expression

Mutation

Promoter

mPGK

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Bin1 delta N-BAR

Plasmid

#168282

Purpose

Express N-BAR-deleted Bin1 mutant (aa 268-476)

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

aa 268-476

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flightless-1-LRR-HA

Plasmid

#21151

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

pcDNA3-Fli-Lrr-HA

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
HOXA9-MSCV-full-GFP

Plasmid

#20977

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

Insert

HOXA9 (Hoxa9 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Full length cDNA with ~1.1kb 3'UTR with 2 pu…

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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