159,557 results

1. pSNAPf-Cox8A Control Plasmid

   Plasmid

   #101129

   Purpose

   Expression of SNAP-Tagged Cox8A in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Insert

   Cox8A (COX8A Human)
   

   Use

   Tags

   SNAP-tag (SNAPf)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1 TaGu N-1F

   Plasmid

   #203762

   Purpose

   For expression of N-flag tagged TaGu R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.22-PGK1-HRE::dLUC

   Plasmid

   #128095

   Purpose

   Hypoxia response element real-time luciferase reporter

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
   

   Use

   Luciferase

   Tags

   drives expression of destabilized luciferase (2CP…

   Expression

   Mammalian

   Mutation

   Promoter

   inserted PGK1 hypoxia response elements (HRE) (x3)

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LD-GA

   Plasmid

   #209862

   Purpose

   Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP A
   

   Use

   Tags

   Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. hGip-R

   Plasmid

   #14942

   Depositor

   Bernard Thorens

   Article

   Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

   Insert

   Gip-R (GIPR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Short form of the receptor

   Promoter

   Available since

   June 7, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-RAB-ICUE

   Plasmid

   #118480

   Purpose

   ddRFP-based single-color biosensor for monitoring cAMP dynamics.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-ICUE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX333-T2A-EGFP

   Plasmid

   #197417

   Purpose

   SpCas9-T2A-EGFP with dual sgRNA cloning backbones

   Depositor

   Yeh-Hsing Lao

   Article

   Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh; U6

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHMM

   Plasmid

   #67717

   Purpose

   Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

   Depositor

   Jeffrey Kieft, Robert Batey

   Article

   Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

   Insert

   Hexahistidine/Maltose binding protein/MS2 coat protein fusion
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNA polymerase

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBA2219

   Plasmid

   #38152

   Depositor

   David Waugh

   Article

   Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

   Insert

   DsbC (dsbC E. coli)
   

   Use

   Tags

   N-terminal leader peptide (cloning artifact)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid

    #208963

    Purpose

    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209723

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

    Plasmid

    #125228

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-TDP-43

    Plasmid

    #235487

    Purpose

    Expression of human TDP-43-EGFP

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. HLA_A0101

    Plasmid

    #165009

    Purpose

    Expression of HLA-A*01:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-A*01:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-PyronicSF/pCMV-myc-nuc

    Plasmid

    #124814

    Purpose

    Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Nuclear Localization Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-mCherry-CAAX

    Plasmid

    #129285

    Purpose

    Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

    Depositor

    Carlos Carmona-Fontaine

    Article

    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

    Insert

    Fluorescent reporter mCherry fused to CAAX polybasic sequence
    

    Use

    Lentiviral

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFP.E227

    Plasmid

    #138249

    Purpose

    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P17_up1691, P20_992, and iP16_3622

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C9orf72

    Plasmid

    #83437

    Purpose

    Expresses GFP-C9orf72 in mammalian cells

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    C9orf72 (C9orf72 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rtn4a-GFP

    Plasmid

    #61807

    Purpose

    Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Rtn4a Stop Codon removed

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pEB1-mGFPmut2

    Plasmid

    #103980

    Purpose

    Plasmid encoding mGFPmut2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mGFPmut2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65A, V68L, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJR85

    Plasmid

    #140095

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. Flag-miniTurbo

    Plasmid

    #124647

    Purpose

    Expresses Flag-miniTurbo fusion for proximity labeling

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234442

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLX304-GW-DCK*-IRES-GFP

    Plasmid

    #176291

    Purpose

    Gateway vector for use in generating POI-DCK* fusion reporter

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-hsyn-GRAB_HA1h

    Plasmid

    #190473

    Purpose

    Express the high-affinity histamine sensor GRAB_HA1h in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine sensor GRAB_HA1h (high affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-GFP

    Plasmid

    #32395

    Depositor

    John T Gray

    Article

    Gray et al Methods Mol Biol. 2011;807:25-46.

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid

    #218628

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-R-iLACCO1

    Plasmid

    #208025

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge