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HI.fate.UB

Plasmid

#191556

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.SFFV

Plasmid

#191554

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Xenopus C-cadherin

Plasmid

#17023

Depositor

Barry Gumbiner

Article

Lee et al Dev Biol. 1995 Oct . 171(2):363-73.

Insert

C-cadherin (cdh3.S Frog)

Use

Xenopus expression

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pTW195b63

Plasmid

#136931

Purpose

Directed evolution of SpCas11

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII(N)-npuN

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pG/ER(G)

Plasmid

#108219

Purpose

Yeast expression vector for human estrogen receptor alpha

Depositor

Didier Picard

Article

Liu et al FEMS Microbiol Lett. 1998 Feb 15;159(2):

Insert

Human estrogen receptor a (ESR1 Human)

Expression

Yeast

Promoter

TDH3

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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p38delta

Plasmid

#29575

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

p38delta (Mapk13 Mouse)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Starting Met replaced with Ala

Available Since

July 13, 2011

Availability

Academic Institutions and Nonprofits only
OeNL_pRSETb

Plasmid

#89529

Purpose

Orange color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-GFP26

Plasmid

#106423

Purpose

Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP26

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pYL322d1/eGFP-C

Plasmid

#183152

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-HASAP1-ST

Plasmid

#138965

Purpose

chemigenetic voltage indicator, soma targeted

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HASAP1-ST

Tags

somatic localization tag

Expression

Mammalian

Promoter

CAG

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_A3003

Plasmid

#91043

Purpose

Module A, Promoter: CmYLCV, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

CmYLCV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_222

Plasmid

#91116

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMH220

Plasmid

#122836

Purpose

dCas9 with GreenGate B-C flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis

Use

Golden gate compatible cloning vector

Mutation

Contains D10A and H840A mutations

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ141A2.0

Plasmid

#99896

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-jAspSnFR3-mRuby3-NLS

Plasmid

#203494

Purpose

Nuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Lentiviral

Tags

Nuclear localization signal and mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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saposin B^W

Plasmid

#161759

Purpose

Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

Depositor

Sam Gellman

Article

Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

Insert

saposin B R38_W

Tags

His-tag

Expression

Bacterial

Mutation

changed Arginine 38 to Tryptophan

Promoter

T7

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-5-enzymatic-domain

Plasmid

#214306

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

aa133-434

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBAV150

Plasmid

#79764

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Jean Greenberg

Article

Vinatzer et al Mol Microbiol. 2006 Oct;62(1):26-44. Epu

Type

Empty backbone

Use

Tetr

Expression

Plant

Promoter

DEX

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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XE69 Xenopus Beta-catenin in GFP

Plasmid

#16839

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pAXY0006

Plasmid

#205575

Purpose

Glucos(1-231)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Inserts

Glucosyltransferase and NpuDnaE(N)_Arabidopsis-codon-OP
CYP76AD1-P2A-DODA

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCO603

Plasmid

#139736

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnLuz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

Luz

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFH66

Plasmid

#131765

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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p3A9cbetaTCR

Plasmid

#69600

Purpose

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pTRANS_223

Plasmid

#91117

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE161

Plasmid

#79453

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Expression

Plant

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Cox8x2-ReNL_pcDNA3

Plasmid

#89533

Purpose

Red color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

CoxVIII X2

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pFH102

Plasmid

#128207

Purpose

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCO600

Plasmid

#139734

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnHisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

Hisps

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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P212

Plasmid

#139718

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_p35S:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHR Clover-VISH2

Plasmid

#188629

Purpose

Fluorescent reporter for SLP76/VISH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

VISH2

Use

Lentiviral

Tags

Clover

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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