We narrowed to 13,037 results for: Mpl

1. pEF5/FRT-DEST EVC2dW-TEV-YFP-Flag

   Plasmid

   #41006

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   Ellis van Creveld syndrome 2 (EVC2 Mouse)
   

   Use

   Tags

   TEV-YFP-Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1177-1220

   Promoter

   Available since

   Nov. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pGEX6P1‐Afu rnhB

   Plasmid

   #108694

   Purpose

   For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Insert

   rnhB (AF_RS03165 Archaeoglobus fulgidus)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. M26I DJ-1 TEV site pET15b

   Plasmid

   #60685

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26I DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26I and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEXP5-NT-CuSeCat EE

   Plasmid

   #42569

   Depositor

   Ivan Korendovych

   Article

   Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8.

   Insert

   mutated calmodulin gene
   

   Use

   Tags

   Polyhistidine (6×His) region, TEV recognition sit…

   Expression

   Bacterial

   Mutation

   changed Phenylalanine 92 to Glutamine acid, Valin…

   Promoter

   T7

   Available since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSG5-FLAG-CaMKK alpha with CaMKKbeta RP domain

   Plasmid

   #33331

   Depositor

   Anthony Means

   Article

   Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

   Insert

   Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   CaMKKalpha RP domain (167-189) was replaced with …

   Promoter

   SV40

   Available since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pUASt-FMW-attB-AthHen1

   Plasmid

   #104958

   Purpose

   Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 drivers

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Use

   Tags

   FLAG Myc

   Expression

   Insect

   Mutation

   Promoter

   Hsp70 promoter

   Available since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 5' Del 6

   Plasmid

   #16054

   Depositor

   Robert Benezra

   Article

   Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

   Insert

   Id1 promoter, 5' deletion (Id1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   -327 to +88 of promoter

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

8. GST-PC-2-CTS

   Plasmid

   #86068

   Purpose

   A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Polycystin-2 (Pkd2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains the region from 1-15

   Promoter

   tac

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 5' Del 1

   Plasmid

   #16049

   Depositor

   Robert Benezra

   Article

   Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

   Insert

   Id1 promoter, 5' deletion (Id1 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   -1372 to +88 of promoter

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

10. 5' Del 4

    Plasmid

    #16052

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter, 5' deletion (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -727 to +88 of promoter

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-HA-REDD1 1-225 (#431)

    Plasmid

    #99927

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal deletion; encodes AA 1-225

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 5' Del 2

    Plasmid

    #16050

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter, 5' deletion (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -1147 to +88 of promoter

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

13. p252 pPr-CREM/CMV-STOP-luc

    Plasmid

    #8406

    Purpose

    Expression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM/lox/luciferase
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    Probasin promoter, CMV

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pEYFP-N1-hRIP3-FL VV458/460PP

    Plasmid

    #61368

    Purpose

    Expresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with VV458/460P mutation (RIPK3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V458 to P and V460to P

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. M26L DJ-1 TEV site pET15b

    Plasmid

    #60686

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26L DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26L and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. KIF5C(1-560)-EF(C)

    Plasmid

    #61675

    Purpose

    Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-EF(C) (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSALECT-Efnb2

    Plasmid

    #59707

    Purpose

    pSALECT with Efnb2 insert

    Depositor

    Christopher Anderson

    Article

    Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

    Insert

    Efnb2 (Efnb2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mKO-Tubulin-6

    Plasmid

    #57850

    Purpose

    Localization: Microtubules, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Article

    Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    mKO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSR04

    Plasmid

    #69151

    Purpose

    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    eGFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    eef-1A.1 (eft-3) and rps-27

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KIF5C(1-560)-IA

    Plasmid

    #61671

    Purpose

    Non-fluorescent, truncated kinesin-1 fused to IA linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-IA (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HOXB4-FLAG-WG-SP65

    Plasmid

    #21087

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    In vitro t/t

    Tags

    Expression

    Mutation

    W to G mutation in YPWM Pbx1 interaction motif, p…

    Promoter

    Available since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pEGFP-N1-hRIP1-FL I539P

    Plasmid

    #61380

    Purpose

    Expresses Human full length RIPK1with I539P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP1with I539P mutation (RIPK1 Human)
    

    Use

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    changed I539 to P

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3 HA c-Jun

    Plasmid

    #237333

    Purpose

    transient overexpression in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

    Insert

    c-Jun (JUN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. plenti-UBC-HA-TMEM230(R78L)

    Plasmid

    #225517

    Purpose

    Lentiviral TMEM230(R78L) expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    R78L

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plenti-UBC-HA-TMEM230(X121PG)

    Plasmid

    #225518

    Purpose

    Lentiviral TMEM230(X121PG) expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    X121PG

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. plenti-UBC-HA-TMEM230(X121W)

    Plasmid

    #225519

    Purpose

    Lentiviral TMEM230(X121W) expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    X121W

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. plenti-UBC-HA-TMEM230(Y29C)

    Plasmid

    #225520

    Purpose

    Lentiviral TMEM230(Y29C) expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al bioRxiv [Preprint]. 2025 Feb 9:2025.02.0

    Insert

    TMEM230 (TMEM230 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    Y29C

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYO359

    Plasmid

    #235750

    Purpose

    Protein expression of FL ScVPS34 and FL ScVPS15-ZZ

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Inserts

    VPS34 (VPS34 Budding Yeast)
    VPS15 (VPS15 Budding Yeast)
    

    Use

    Tags

    3xTEV-ZZ

    Expression

    Yeast

    Mutation

    S2A, aa 3-887 and T134A, I851R

    Promoter

    GAL-TDH3

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYO1006

    Plasmid

    #235724

    Purpose

    Expression of FL human Beclin 1 in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

    Insert

    Beclin 1 (BECN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. OPEN-A*24:02

    Plasmid

    #235133

    Purpose

    E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*24:02 (HLA-A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. OPEN-HLA-A*01:01

    Plasmid

    #235134

    Purpose

    E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV PHP.eB-CAG-Venus

    Plasmid

    #233695

    Purpose

    AAV expression of Venus from CAG promoter

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Venus
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSMPPv2-Tau-RING

    Plasmid

    #233688

    Purpose

    Expression of Tau-RING protein in mammalian cells

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Tau protein fused to TRIM21 RING domain
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSM41

    Plasmid

    #235728

    Purpose

    Protein expression of FL His6- human VPS34 in bacteria

    Depositor

    Roger Williams

    Article

    Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

    Insert

    VPS34 (PIK3C3 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRDA_052_HygroR

    Plasmid

    #228937

    Purpose

    Plasmid for cloning of custom gRNA using EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231346

    Purpose

    Self complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FAM177A1-Halo

    Plasmid

    #224591

    Purpose

    For expression of FAM177A1-Halo in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    FAM177A1 (FAM177A1 Human)
    

    Use

    Tags

    HALO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ZFPL1-GFP

    Plasmid

    #224590

    Purpose

    For expression of ZFPL1-GFP in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    ZFPL1 (ZFPL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FAM177A1-SNAP

    Plasmid

    #224592

    Purpose

    For expression of FAM177A1-SNAPTag in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    FAM177A1 (FAM177A1 Human)
    

    Use

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMRX-IP-hWIPI3-3×FLAG

    Plasmid

    #215511

    Purpose

    Expresses FLAG tagged human WIPI3.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    WD-repeat protein interacting with phosphoinositides 3 (WDR45B Human)
    

    Use

    Retroviral

    Tags

    3×FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDONR221-DXO

    Plasmid

    #177992

    Purpose

    For gateway cloning of DXO

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    DXO (DXO Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDONR221-PARP11

    Plasmid

    #184469

    Purpose

    For gateway cloning of PARP11

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP11 (PARP11 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

    Plasmid

    #215910

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332D, S1346D, S1616D, S169…

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

    Plasmid

    #215911

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332A, S1346A, S1616A, S169…

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

    Plasmid

    #215905

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332A, S1346A, S1616A, S169…

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pTE5418_pMBP-GlcR

    Plasmid

    #221192

    Purpose

    Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    glcR (pden4400 from Paracoccus denitrificans)
    

    Use

    Tags

    10x His tag with E. coli maltose binding protein …

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and T7 promoter-lacO

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMx/ATP9A-HA

    Plasmid

    #209230

    Purpose

    Mammalian expression of ATP9A

    Depositor

    Hye-Won Shin

    Article

    Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBK1302-AAV-EFSNC-dCjCas9-KRAB

    Plasmid

    #223147

    Purpose

    Expression of KRAB with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBK1312-AAV-EFSNC-dSaCas9-KRAB

    Plasmid

    #223157

    Purpose

    Expression of KRAB with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

    Plasmid

    #223163

    Purpose

    2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge