We narrowed to 11,959 results for: pcDNA

1. pcDNA3.1(+)-TTF-1-AD2-deletion

   Plasmid

   #75266

   Purpose

   Expresses a deletion mutant of human TTF-1 lacking the activation domain 2

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   TTF-1 (NKX2-1 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   AD2 (activation domain 2) deleted

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-FLAG-Lin28B-mCCHC

   Plasmid

   #51375

   Depositor

   Narry Kim

   Article

   Heo et al Cell. 2009 Aug 21;138(4):696-708. doi: 1

   Insert

   Lin28B (LIN28B Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   CCHC domain mutant (H137A, H159A)

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5/FRT/TO GFP DNAJB7

   Plasmid

   #19503

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB7 (DNAJB7 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-5'UTR-MAVS(KaganD88)

   Plasmid

   #52005

   Purpose

   Expresses the human MAVS CDS and contains the 5'UTR sequense

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   5utr-MAVS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5-FRT-TetO-fSNAPc

   Plasmid

   #106986

   Purpose

   open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   fSNAP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 3xFLAG TPP1 88-249

   Plasmid

   #53549

   Purpose

   mammalian expression of TPP1 OB-fold domain

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   aa 88-249

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-PARL-D319A-FLAG C-t

   Plasmid

   #191958

   Purpose

   Expression of Presenilin Associated Rhomboid Like (PARL) D319A mutant

   Depositor

   Luca Scorrano

   Insert

   PARL D319A
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D319A

   Promoter

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1+_LN-HA-AGO2-5xA

   Plasmid

   #92010

   Purpose

   Expresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   Lambda-N-HA

   Expression

   Mammalian

   Mutation

   S824A, S828A, T830A, S831A, S834A (5xA)

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 3440 pcDNA3 Bid p22

   Plasmid

   #8774

   Depositor

   Stanley Korsmeyer

   Article

   Zha et al Science 2000 Dec 1;290(5497):1761-5.

   Insert

   Bid p22 wt (Bid Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA4/HisMaxB-YAP1-S163A

    Plasmid

    #18989

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein S163A mutant (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Serine 163 changed to Alanine,

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.2/V5 hsa-mir-499

    Plasmid

    #26320

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-499 (MIR499A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-HA2-Cul4A-deltaROC

    Plasmid

    #20713

    Depositor

    Yue Xiong

    Article

    McCall et al Mol Cell Biol. 2008 Sep . 28(18):5621-33

    Insert

    Cullin 4A (CUL4A Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Deletion of residues 594 to 612 from N-terminus

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-hRIPK1-Flag-K377R

    Plasmid

    #112489

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K377R

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-myc-AP1S3 F4C

    Plasmid

    #58293

    Purpose

    mammalian expression of AP1S3 F4C mutant

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Use

    Tags

    6xHis and myc

    Expression

    Mammalian

    Mutation

    F4C: c.11T>G, p.Phe4Cys

    Promoter

    CMV

    Available since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1(+) Laccase2 MCS-mFndc3b

    Plasmid

    #126443

    Purpose

    Expresses mouse Fndc3b circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

    Insert

    Fndc3b (Fndc3b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    circFndc3b exons are separated by a miniature int…

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. delta-BRIP1-pcDNA3.1/MycHis

    Plasmid

    #19042

    Depositor

    Ronny Drapkin

    Article

    De Nicolo et al Clin Cancer Res. 2008 Jul 15. 14(14):467

    Insert

    delta BRIP1 (BRIP1, BRCA1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    This is a novel BRIP1 germline mutation that cons…

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

17. cRel RHD cFlag pcDNA3

    Plasmid

    #20016

    Depositor

    Stephen Smale

    Insert

    c-Rel (Rel Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Contains only the Rel Homology Domain of c-Rel. I…

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pCDNAFlagMenin(S394A, T397A, S399A)

    Plasmid

    #32081

    Depositor

    Matthew Meyerson

    Article

    Francis et al PLoS One. 2011 . 6(1):e16119.

    Insert

    Menin
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S394A,T397A, S399A

    Promoter

    CMV

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.2 V5-DEST mir-1-1 reporter

    Plasmid

    #46646

    Purpose

    expression of query pri-miRNA

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir-1-1 reporter
    

    Use

    RNAi; Expression of query pri-mirna sequences in …

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-CAML-Myc-His

    Plasmid

    #22295

    Depositor

    Erik Lillehoj

    Article

    Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

    Insert

    calcium modulating ligand (CAMLG Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3.2/V5 mmu-mir-153

    Plasmid

    #26330

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-153 (Mir153 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA4/HisMaxB-YAP1-S164A

    Plasmid

    #18990

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein S164A mutant (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Serine 164 changed to Alanine

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA-CasE-p300-Flag

    Plasmid

    #106275

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE-p300
    

    Use

    CRISPR

    Tags

    E tag, NLS and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-DDX11-Flag-KAK

    Plasmid

    #120729

    Purpose

    Expression of a Flag-tagged version of DDX11 KAK mutant in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 KAK mutant (DDX11 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Substitution of E201, Y202 and E203 with K, A and…

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-myc-MmUbc7mut/2777

    Plasmid

    #11489

    Depositor

    Allan Weissman

    Article

    Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

    Insert

    MmUbc7 (Ube2g2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    cys 889 mutated to ser

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3.1/Xpress beta4 1355T

    Plasmid

    #16040

    Depositor

    Filippo Giancotti

    Article

    Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    6xHis and Xpress

    Expression

    Mammalian

    Mutation

    1355T: amino acids 1-1355

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3.2 mir-1-1 reporter mir16-1 wt

    Plasmid

    #46683

    Purpose

    Expresses wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1 wt (MIR16-1 Human, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA-FRT-FAK-kinase0

    Plasmid

    #59122

    Purpose

    FAK fret control sensor without FERM domain with no linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3 Flag MFF iso1 WT

    Plasmid

    #74388

    Purpose

    Flag tagged human MFF isoform 1

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta exon 1 (1-26)

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA3.1-myc-AP1S3 R33W

    Plasmid

    #58294

    Purpose

    mammalian expression of AP1S3 R33W mutant

    Depositor

    Francesca Capon

    Article

    Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

    Insert

    AP1S3 (AP1S3 Human)
    

    Use

    Tags

    6xHis and myc

    Expression

    Mammalian

    Mutation

    R33W: c.97C>T,p.Arg33Trp

    Promoter

    CMV

    Available since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

31. Nano-lantern-H2B/pcDNA3

    Plasmid

    #51971

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    human H2B

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3 merlin 308-590

    Plasmid

    #11627

    Depositor

    Vijaya Ramesh

    Article

    Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

    Insert

    merlin (NF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Carboxy-terminal half of merlin isoform 2.

    Promoter

    Available since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

33. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid

    #84634

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    JNKsub(TA)DD-YPet-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.2/V5 mmu-mir-138-1

    Plasmid

    #26328

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-138-1 (Mir138-1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA3-HA-BHLHE40(S243A/E248A)

    Plasmid

    #110155

    Purpose

    Expression of human BHLHE40 (S243A/E248A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

    Insert

    BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S243A/E248A

    Promoter

    CMV

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4

    Plasmid

    #112839

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Grb2 (Grb2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SH3 (N-term) domain of Grb2

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.2/V5 hsa-mir-483

    Plasmid

    #26315

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-483 (MIR483 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA3-FLAG-HA-CAD S1900A

    Plasmid

    #46240

    Purpose

    FLAG-HA-CAD with S1900 mutated to alanine

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Serine 1900 changed to Alanine (S1900A)

    Promoter

    CMV

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

    Plasmid

    #80272

    Purpose

    mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5-MIS12-LAP-BUB1

    Plasmid

    #115904

    Purpose

    Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

    Insert

    MIS12-BUB1 (MIS12 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    BUB1 fused to MIS12

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge