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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,962 results for: tet on

Showing: 6101 - 6150 of 6962 results

  2. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64986-plasmid-map-sequence-id-109956

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  7. JAB3312

    Plasmid
    #190406
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    190406-plasmid-map-sequence-id-391159

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  8. JAB2602

    Plasmid
    #190389
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    190389-plasmid-map-sequence-id-393424

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  12. pSTAP-seq_human-4xUAS

    Plasmid
    #125150
    Purpose
    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    4 x upstream activating sequence (UAS)
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    125150-plasmid-map-sequence-id-254778

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  23. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Calvin Tiengwe
    Article
    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Promoter
    Pol II readthrough transcription after integration
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210224-plasmid-map-sequence-id-415797

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  24. pRJPaph-lacZYA

    Plasmid
    #67037
    Purpose
    Plasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    lacZYA
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67037-plasmid-map-sequence-id-115553

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  26. pBAD-tdTEVDB_C-15A/G4C

    Plasmid
    #177205
    Purpose
    Permuted td intron with evolved initiation sequence for the expression of circular mRNA
    Depositor
    Stephen Fried
    Article
    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175
    Insert
    Permuted GFP with evolved initiation sequence for circular mRNA
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Permuted sfGFP after residue 52 & Changed Cyt…
    Promoter
    araBAD
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177205-plasmid-map-sequence-id-373215

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  28. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid
    #185687
    Purpose
    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).
    Depositor
    Peter Walter
    Article
    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75
    Insert
    ERmembrane-GST-2xHaloTag-KKMP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    185687-plasmid-map-sequence-id-366415

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  32. pRJPaph-gusA

    Plasmid
    #67036
    Purpose
    Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    gusA
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67036-plasmid-map-sequence-id-115551

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  33. JAB2603

    Plasmid
    #190390
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    190390-plasmid-map-sequence-id-393426

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  35. mCer-Vamp2(qv/fw)

    Plasmid
    #53238
    Purpose
    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Use
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Mutated Q76 to V; F77 to W.
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pENV8(GAAA)-GFPuv

    Plasmid
    #70128
    Purpose
    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop
    Depositor
    Robert Batey
    Article
    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:
    Insert
    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70128-plasmid-map-sequence-id-130022

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  39. pRJPaph-mTq2

    Plasmid
    #67031
    Purpose
    Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    mTurqoise2
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67031-plasmid-map-sequence-id-115541

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  42. IBMc071

    Plasmid
    #161943
    Purpose
    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161943-plasmid-map-sequence-id-327544

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  43. pTL-Bag3-gRNA3

    Plasmid
    #196254
    Purpose
    Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196254-plasmid-map-sequence-id-385944

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  44. pTL-Bag2-gRNA2

    Plasmid
    #196252
    Purpose
    Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196252-plasmid-map-sequence-id-385943

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  45. pTL-Bag1-gRNA1

    Plasmid
    #196251
    Purpose
    Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196251-plasmid-map-sequence-id-385941

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  46. pTL-Bag4-gRNA4

    Plasmid
    #196255
    Purpose
    Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs
    Depositor
    Bing Yang
    Article
    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1
    Insert
    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196255-plasmid-map-sequence-id-385931

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  50. pCS5

    Plasmid
    #55750
    Purpose
    Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.
    Depositor
    Matthew Bennett
    Article
    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50
    Insert
    KanR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Residues 59-264 of KanR
    Promoter
    T7-lac
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    55750-plasmid-map-sequence-id-86407

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Showing: 6101 - 6150 of 6962 results