We narrowed to 18,759 results for: nls

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pJZ012_EFS-Cas12a2-puro

Plasmid

#257716

Purpose

Express SuCas12a2 in mammalian cell lines; Puromycin selection.

Depositor

Jennifer Doudna

Publication

Zeng et al Nature. 2026 Jun 8. doi: 10.1038/s41586-

Insert

SuCas12a2

Use

CRISPR

Tags

NLS-FLAG

Expression

Mammalian

Promoter

EF-1alpha

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-KRAB

Plasmid

#191314

Purpose

piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Publication

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

KRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Publication

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Promoter

U6 and hsp70

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSAD-F3-NPEST-iCRE-2A-mCherryPEST

Plasmid

#99608

Purpose

Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

Depositor

Marco Tripodi

Publication

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

iCRE
mCherry

Use

Unspecified

Tags

PEST

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE2 Lnp-mCherry

Plasmid

#86687

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Publication

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

LNPK (LNPK Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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DHFR-SpCas9-DHFR

Plasmid

#85447

Purpose

Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Publication

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-SpCas9-DHFR

Use

AAV

Tags

Destabilized domain (C-terminus version) of E.col…

Expression

Mammalian

Promoter

Cbh

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PGK-AAVS1ZFNL

Plasmid

#60916

Purpose

encodes zinc finger nuclease specific to AAVS1 locus (left)

Depositor

Paul Gadue

Publication

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

AAVS1-ZFNL

Promoter

PGK

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EMM65

Plasmid

#49042

Purpose

Backbone contains SHD/C 0.5 Domain TALE N-terminal: TAL C-terminus: LSD1: Isoform 2

Depositor

Bradley Bernstein, Eric Mendenhall

Publication

Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

Type

Empty backbone

Use

Tale lsd1 expression vector

Tags

LSD1

Expression

Mammalian

Promoter

EF1 alpha

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pThy1-Brainbow3.1

Plasmid

#45178

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pThy1-Brainbow3.1

Use

Linearized for transgenic mouse injection

Promoter

Thy1

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-GAAZFP-FokI-RR

Plasmid

#18754

Depositor

Scot Wolfe

Publication

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LTRi

Plasmid

#15913

Depositor

James Collins

Publication

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Oct. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pLVTH-sip53

Plasmid

#12239

Depositor

Didier Trono

Publication

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

shRNA against p53 (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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VP16-ER beta (long)

Plasmid

#11352

Depositor

Donald McDonnell

Publication

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTRIPZ-NLS-PTEN

Plasmid

#206984

Purpose

Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

Depositor

Ming Chen

Publication

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

tet-on promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TO-CasRx(NLS)-T2A-mCherry

Plasmid

#242433

Purpose

CasRx (NLS-RfxCas13d-NLS) for RNA targeting under the CMV-TO promoter (CMV with two Tet-Operators)

Depositor

Diego Di Bernardo

Publication

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

CasRx

Use

CRISPR

Tags

Tag / Fusion Protein: T2A mCherry

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-SpRY

Plasmid

#159979

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Publication

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PE2-SpRY

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ErkKTR-irFP

Plasmid

#111510

Purpose

Reporter of Erk activity

Depositor

Jared Toettcher

Publication

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

ERK KTR (Mapk1 Mouse)

Use

Lentiviral

Tags

iRFP

Promoter

SSFV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Publication

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

Plasmid

#107270

Purpose

Expresses Cas9 upon induction with doxicycline.

Depositor

Pablo Menéndez

Publication

Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

hSpCas9
rtTA
bGH polyA-SV40polyA

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG and TRE

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAdSh.PGK.Cas9

Plasmid

#58253

Purpose

Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

Depositor

Manuel Goncalves

Publication

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

Human codon-optimized S. pyogenes Cas9

Use

Adenoviral and CRISPR

Expression

Mammalian

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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