We narrowed to 29,541 results for: REP

1. pFA6a-link-ymScarletI-URA3

   Plasmid

   #168055

   Purpose

   Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymScarletI
   

   Use

   Tags

   ymScarletI

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6_MBP_FXR1P

   Plasmid

   #198694

   Purpose

   Expression of human FXR1 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR1P (FXR1 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-hSyn-EGFP

   Plasmid

   #114215

   Purpose

   Lentiviral vector (control) that targets expression of EGFP to neurons

   Depositor

   Xue Han

   Article

   Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   human synapsin (hSyn)

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMT-flp

   Plasmid

   #67274

   Purpose

   Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

   Depositor

   Mark Liles

   Article

   Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

   Insert

   tetA, flp, repA101, oriR101
   

   Use

   Flp recombinase plasmid, temperature sensitive

   Tags

   His-SUMO (flp)

   Expression

   Mutation

   Promoter

   tetA promoter of plasmid pACYC184 origin

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9 fused to nanobody (anti-beta catenin)

   Plasmid

   #186420

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-nanobody fusion (anti-beta catenin)
   

   Use

   CRISPR

   Tags

   6His

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNCS-mRuby3

   Plasmid

   #74234

   Purpose

   Constitutive expression of mRuby3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   mRuby3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-hEF1a-MG

   Plasmid

   #175504

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Apex2-Venus-CAAX

   Plasmid

   #168511

   Purpose

   Expression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Sci Rep. 2018 Sep 27;8(1):14491. doi: 10

   Insert

   Apex2
   

   Use

   Tags

   Venus-CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK462 (Hygro-P2A-mAB)

   Plasmid

   #214388

   Purpose

   mAID-BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   Hygro-P2A-mAID-BromoTag
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-ymTq2-URA3

    Plasmid

    #168057

    Purpose

    Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Use

    Tags

    ymTurquoise2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMT-3P2A

    Plasmid

    #111772

    Purpose

    Quadcistronic construct: Co-express four genes by 3 P2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. modRNAc0-p53DD

    Plasmid

    #176902

    Purpose

    IVT template plasmid for making p53DD modRNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    p53DD
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p.UTA.3.0 Empty

    Plasmid

    #82447

    Purpose

    [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLSSmOrange-mKate2

    Plasmid

    #99868

    Purpose

    Measure FRET between LSSmOrange and mKate2

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    LSSmOrange
    

    Use

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSC101-GFPmut2-mScarlet-I TAA

    Plasmid

    #208187

    Purpose

    nonsense mistranslation, TAA stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Insertion 6TAA*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5155

    Plasmid

    #175391

    Purpose

    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

    Depositor

    Michael Wiles

    Article

    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting; Cloning vector, to be used to de…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK445 (BSD-P2A-BromoTag)

    Plasmid

    #214375

    Purpose

    BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-BromoTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDest-635

    Plasmid

    #159582

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTXTL-T7max-FLuc

    Plasmid

    #188311

    Purpose

    Expression of Firefly luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Firefly luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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