58,072 results

1. TFORF1932

   Plasmid

   #142292

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUN (JUN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

   Plasmid

   #184464

   Purpose

   Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA6 asyn WT

   Plasmid

   #107425

   Purpose

   Mammalian expression of human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC-hrDNA-12.0

   Plasmid

   #206320

   Purpose

   A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

   Depositor

   Brian McStay

   Article

   van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

   Insert

   Intergenic spacer of human rDNA repeat
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TRUPATH Triple Gai1

   Plasmid

   #196048

   Purpose

   Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai1-RLuc8 (GNAI1 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-HxB.L

   Plasmid

   #65414

   Purpose

   contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

   Depositor

   Harold Erickson

   Article

   Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

   Insert

   TNC large splice variant (TNC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

   Plasmid

   #71293

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV4-p52

   Plasmid

   #23289

   Depositor

   Warner Greene

   Article

   Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

   Insert

   p52 (NFKB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.2 V5-DEST 3XFlag-SRSF3

   Plasmid

   #46736

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   SRSF3 (SRSF3 Human)
   

   Use

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLPCX-PECAMTS

    Plasmid

    #45850

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    PECAM1 (PECAM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ODD-Luciferase-pcDNA3

    Plasmid

    #18965

    Depositor

    William Kaelin

    Article

    Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

    Insert

    ODD-Firefly Luciferase (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HIF1a ODD sequence fused in frame to luciferase O…

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-Lrrc59

    Plasmid

    #226565

    Purpose

    Encodes Lrrc59 protein laebeled with mCherry

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmiRGLO Dual-Luciferase-PDAP1-3'UTR

    Plasmid

    #182251

    Purpose

    Human PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQE-60 Grx1-roGFP2-His

    Plasmid

    #64799

    Purpose

    Bacterial expression of His-tagged Grx1-roGFP2

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    His and roGFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TFORF3137

    Plasmid

    #144613

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HEY1 (HEY1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-EGFP-HaloTag2

    Plasmid

    #41742

    Depositor

    Craig Crews

    Article

    Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

    Insert

    Halotag 2
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-CRY2clust

    Plasmid

    #105624

    Purpose

    Expresses improved CRY2PHR homo-oligomerization

    Depositor

    Won Do Heo

    Article

    Park et al Nat Commun. 2017 Jun 23;8(1):30. doi: 10

    Insert

    mCherry-CRY2PHR-9aa (CRY2 Human, Mustard Weed)
    

    Use

    Tags

    9 amino acids

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mH2A1.1-CT-GFP

    Plasmid

    #45167

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-Ub KO

    Plasmid

    #11934

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin KO (RBX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    All 7 lysines of ubiquitin mutated to arginines.

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PPRE-H2B-eGFP

    Plasmid

    #84393

    Purpose

    Fluorescent reporter for PPARgamma activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

    Insert

    PPARG response element (PPARG Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    PPRE

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge