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tetO-ALN

Plasmid

#43918

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B, NR4A2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

6xHis and V5

Expression

Mammalian

Mutation

Gylcine-serine-glycine (GSG) linker followed by v…

Promoter

TRE promoter, Tet-ON

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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dsRed-cent2

Plasmid

#29523

Depositor

Joseph Gleeson

Article

Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

Insert

Cent2 (CETN2 Human)

Tags

dsRed

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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AP2u2-mCherry

Plasmid

#27672

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Ap2m1 (Ap2m1 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP

Plasmid

#19444

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pShuttle-Cre-HA

Plasmid

#16583

Depositor

Bert Vogelstein

Article

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

cre recombinase (cre P1 bacteriophage)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-hPIASy

Plasmid

#15208

Depositor

Ke Shuai

Article

Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

Insert

PIASy (PIAS4 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-GFP_2.0

Plasmid

#11375

Purpose

Retroviral construct for miRNA expression

Depositor

Chang-Zheng Chen

Article

Chen et al Science. 2004 Jan 2. 303(5654):83-6.

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA 14-3-3 sigma

Plasmid

#11946

Depositor

Michael Yaffe

Article

Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

Insert

Stratifin (SFN Human)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

Plasmid

#141383

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLBH531_MBP-Cas14a1 expression

Plasmid

#112500

Purpose

Expresses MBP fused to Cas14a1

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_WT_mCherry

Plasmid

#84020

Purpose

expression of SRSF2 in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-BRD2

Plasmid

#65376

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD2 (BRD2 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Dendra2-Lifeact-7

Plasmid

#54694

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

Lifeact-Dendra2

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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