We narrowed to 5,634 results for: pcas

1. pX330-MM002-ZFP143-C-terminal-sgRNA

   Plasmid

   #212706

   Purpose

   Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

   Depositor

   Elzo de Wit

   Insert

   ZFP143 C-terminal sgRNA (Zfp143 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lenti-Cas9-Exo-Blast-BFP

   Plasmid

   #196721

   Purpose

   Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-Exo1-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330.orup

   Plasmid

   #110404

   Purpose

   CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Or46a-Gal4

   Plasmid

   #63179

   Purpose

   Expresses Gal4 under control of the Drosophila melanogaster Or46a odorant receptor promoter

   Depositor

   Leslie Vosshall

   Article

   Fishilevich et al Curr Biol. 2005 Sep 6;15(17):1548-53.

   Insert

   Or46a promoter
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. CJ31 (miR-1006 minigene)

   Plasmid

   #17768

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   dme-miR-1006 minigene
   

   Use

   Drosophila p-element vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pBBR-Cas9ΔyqhD-HA500

   Plasmid

   #220369

   Purpose

   A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

   Depositor

   Yilan Liu

   Inserts

   SpCas9
   sgRNA for yqhD in P. putida KT2440
   

   Use

   CRISPR; Broad host range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCX137

   Plasmid

   #229212

   Purpose

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCX459

   Plasmid

   #229214

   Purpose

   CRISPR array plasmid for MAP1B (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for MAP1B (Spacers 1-24)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCX460

   Plasmid

   #229215

   Purpose

   CRISPR array plasmid for MAP1B (Spacers 25-48)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for MAP1B (Spacers 25-48)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-GFP

    Plasmid

    #11153

    Purpose

    Mammalian expression vector for expression of GFP (CMV promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    CMV promoter-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAG-FR-GECO1c

    Plasmid

    #163682

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1c

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CAG-FR-GECO1a

    Plasmid

    #163681

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1a

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNDamMyc

    Plasmid

    #59217

    Purpose

    Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

    Depositor

    Bas van Steensel

    Article

    van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

    Type

    Empty backbone

    Use

    Damid

    Tags

    Dam and Myc

    Expression

    Insect

    Mutation

    Promoter

    Drosophila heat-shock promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCralbp-DsRed

    Plasmid

    #11158

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    Cralbp promoter (Rlbp1 Mouse)
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-ABE-P48R-UGI

    Plasmid

    #161817

    Purpose

    Expresses ABE-P48R-UGI in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJRT.Agro.CLCrVB1.3

    Plasmid

    #37974

    Depositor

    Niki Robertson

    Article

    Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

    Insert

    pJRTCLCrVB1.3
    

    Use

    Agrobacterium binary

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTALYM3

    Plasmid

    #47874

    Purpose

    Destination plasmid with mClover for Golden Gate reaction of TALE assembly

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Type

    Empty backbone

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMycDam

    Plasmid

    #59218

    Purpose

    Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam

    Depositor

    Bas van Steensel

    Article

    van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

    Type

    Empty backbone

    Use

    Damid

    Tags

    Dam and Myc

    Expression

    Insect

    Mutation

    Promoter

    Drosophila heat-shock promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKF295

    Plasmid

    #72871

    Purpose

    expression of improved FLP recombinase

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    FLP recombinase (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    P2->S, added NLS

    Promoter

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SV40 basal promoter-LacZ

    Plasmid

    #18816

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    SV40 basal promoter-LacZ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pTC147

    Plasmid

    #70013

    Purpose

    T-DNA vector with Kanamycin resistance carrying a 35S:ANT1 over-expression cassette.

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    35S:ANT1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BA-Wnt10b

    Plasmid

    #1831

    Depositor

    Philip Leder

    Article

    Lane et al Oncogene 1997 Oct;15(18):2133-44.

    Insert

    Wnt10b (Wnt10b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

23. F3/-2.5CAT

    Plasmid

    #25425

    Depositor

    Charles P. Emerson Jr.

    Article

    Goldhamer et al Science. 1992 Apr 24. 256(5056):538-42.

    Insert

    Myo D Promoter + F3 (MYOD1 Human)
    

    Use

    Reporter construct

    Tags

    CAT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

24. -2.5CAT

    Plasmid

    #25424

    Depositor

    Charles P. Emerson Jr.

    Article

    Goldhamer et al Science. 1992 Apr 24. 256(5056):538-42.

    Insert

    Myo D Promoter (MYOD1 Human)
    

    Use

    Reporter construct

    Tags

    CAT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pEGB3_alpha2 35:PhyB:VP16 (GB2745)

    Plasmid

    #160630

    Purpose

    TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

    Depositor

    Diego Orzaez

    Insert

    PhyBVP16
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXN-FBLWLF

    Plasmid

    #26262

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    Multiple cloning site
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FoRC.PGa

    Plasmid

    #84584

    Purpose

    Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.

    Depositor

    Francesca Pignoni

    Article

    Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FoRC.PRa

    Plasmid

    #84585

    Purpose

    Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.

    Depositor

    Francesca Pignoni

    Article

    Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FoRC.PRm

    Plasmid

    #84587

    Purpose

    Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive RFP reporter.

    Depositor

    Francesca Pignoni

    Article

    Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CJ34 (miR-1003 3'ssAG->GA)

    Plasmid

    #17770

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    dme-miR-1003 minigene 3'ssAG->GA
    

    Use

    Drosophila p-element vector

    Tags

    Expression

    Mutation

    splice site mutation

    Promoter

    Available since

    May 12, 2008

    Availability

    Academic Institutions and Nonprofits only