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Showing: 6251 - 6300 of 157643 results

  2. pEvol-MjaYRS

    Plasmid
    #153557
    Purpose
    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli
    Depositor
    Huiwang Ai
    Article
    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038
    Inserts
    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    glnS and pBAD
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    153557-plasmid-map-sequence-id-306290

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  5. pL97

    Plasmid
    #78576
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter
    Depositor
    Xuming Mao
    Article
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ermEp* promoter from S. erythraea
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    78576-plasmid-map-sequence-id-421926

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  6. pGL3 Enhancer Vector

    Plasmid
    #212938
    Purpose
    Firefly luciferase vector for investigating regions controlling transcription
    Depositor
    Debrya Groskreutz
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212938-plasmid-map-sequence-id-419193

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  8. GFP-PS9

    Plasmid
    #177944
    Purpose
    Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    eGFP (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177944-plasmid-map-sequence-id-348205

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  9. pH2B-cherryFP

    Plasmid
    #51001
    Purpose
    Expresses H2B-chFP
    Depositor
    Rusty Lansford
    Insert
    CherryFP
    Use
    Tags
    histone2B
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51001-plasmid-map-sequence-id-77770

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  14. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71667-plasmid-map-sequence-id-243369

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  16. eGFP-hCOL1A1

    Plasmid
    #140110
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag, K20…
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140110-plasmid-map-sequence-id-276323

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  23. pJAT30

    Plasmid
    #204294
    Purpose
    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204294-plasmid-map-sequence-id-405490

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  33. pTJK299

    Plasmid
    #138000
    Purpose
    SIX1 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    SIX1 (SIX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138000-plasmid-map-sequence-id-274994

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  35. FUGW-APPSwe-T2A-Hygro

    Plasmid
    #190805
    Purpose
    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance gene
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Swedish (KM670/671NL)
    Promoter
    Ubi C
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    190805-plasmid-map-sequence-id-372847

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  36. pcDNA3-Casp2-Flag

    Plasmid
    #11811
    Depositor
    Guy Salvesen
    Insert
    Caspase-2 (CASP2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only
    11811-plasmid-map-sequence-id-299764

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  40. Nxn-1 beta Flag/pCMV

    Plasmid
    #197339
    Purpose
    Plasmid expressing an antigen targeting Neurexin 1beta
    Depositor
    Thomas Südhof
    Insert
    Neurexin 1beta (Nrxn1 Rat)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197339-plasmid-map-sequence-id-401579

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  48. mEmerald-Homer1-C-18

    Plasmid
    #54120
    Purpose
    Localization: Membrane Adaptor, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Homer1 (Homer1 Rat)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  50. pTH847-gst-optsc

    Plasmid
    #96908
    Purpose
    Plasmid for yeast expression of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon optimised glutathione-S transferase
    Use
    Tags
    8xhistidine
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96908-plasmid-map-sequence-id-206073

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Showing: 6251 - 6300 of 157643 results