161,873 results

1. pCMV_SEPT2-mApple

   Plasmid

   #180319

   Purpose

   mammalian expression of human SEPT2 fused to monomeric Apple

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB CAG-mTagBFP2-T2A-iCreERT2

   Plasmid

   #177308

   Purpose

   CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 Connexin43-GFP-APEX2

   Plasmid

   #49385

   Purpose

   Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Connexin43 - EGFP - APEX2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS73

   Plasmid

   #110629

   Purpose

   CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating

   Depositor

   Regine Kahmann

   Article

   Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d

   Inserts

   short U6 promoter
   cas9
   tnos terminator
   

   Use

   CRISPR; Self-replicating in ustilago maydis, conf…

   Tags

   N-terminal NLS, C-terminal HA-tag +NLS

   Expression

   Bacterial

   Mutation

   Streptococcus pyogenes gene codon-optimized for U…

   Promoter

   U. maydis hsp70 promoter (Kronstad and Leong, 198…

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-HRD1

   Plasmid

   #231688

   Purpose

   Expresses human HRD1 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   HRD1 (SYVN1 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUGW-eGFP-ZEB1 3'UTR

   Plasmid

   #81018

   Purpose

   FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

   Depositor

   Jeffrey Rosen

   Article

   Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

   Insert

   Zeb1 3'UTR (UTS2R Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSYN-miRFP670nano3-WPRE-hGHpA

   Plasmid

   #184688

   Purpose

   Mammalian expression, miRFP670nano3

   Depositor

   Vladislav Verkhusha, Axel Nimmerjahn

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSYN

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCKan-yibDp-GFPuv

   Plasmid

   #127078

   Purpose

   Low phosphate inducible yibD gene promoter expressing GFPuv reporter

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.23-GW

   Plasmid

   #60323

   Purpose

   This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUGW-APOE-E4-T2A-mCherry

    Plasmid

    #190809

    Purpose

    Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E4

    Promoter

    Ubi C

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRS-XylS-dCas9

    Plasmid

    #220196

    Purpose

    mini-Tn7 dCas9 expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    XylS/pM

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLKO.1-UPF1-CDS

    Plasmid

    #136037

    Purpose

    UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid

    #234954

    Purpose

    Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid

    #234951

    Purpose

    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-AKAR4-NES

    Plasmid

    #64727

    Purpose

    Cytosol-targeted, FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

    Insert

    AKAR4-NES
    

    Use

    Tags

    Cerulean, NES, and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

    Plasmid

    #207863

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICSL11024 (pICH47732::NOSp-NPTII-OCST)

    Plasmid

    #51144

    Purpose

    Confers KAN resistance in planta

    Depositor

    Jonathan D Jones

    Insert

    NOSp-NPTII-OCST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. CD40L-SrtA

    Plasmid

    #121167

    Purpose

    pMP71 vector expressing Tomato - P2A - CD40L - SrtA - flag

    Depositor

    Gabriel Victora

    Article

    Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

    Insert

    CD40L-SrtA
    

    Use

    Retroviral

    Tags

    Tomato and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPSU250

    Plasmid

    #173837

    Purpose

    Expresses 250 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBT281

    Plasmid

    #122611

    Purpose

    express evoAPOBEC1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. C1-CFP-Lyn11

    Plasmid

    #155234

    Purpose

    membane marker

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lyn11 membrane targeted CFP. Membrane control
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1.Gα11-LgB97

    Plasmid

    #134361

    Purpose

    Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα11-LgB97 (GNA11 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMRXIB-mRuby3-LC3

    Plasmid

    #221738

    Purpose

    Stable expression of mRuby3-LC3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MXS_BidirectionalCAG

    Plasmid

    #78175

    Purpose

    Bidirectional CAG promoter (compatible with the MXS Chaining Kit)

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    Bidirectional CAG Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1(+)/Puro/TagRFP-LL5b

    Plasmid

    #112828

    Purpose

    transient/stable overexpression

    Depositor

    Joshua Sanes, Tomasz Prószyński

    Article

    Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

    Insert

    LL5b (Phldb2 Mouse)
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tTRE, hPGK

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. JD171

    Plasmid

    #229483

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PG13-mCherry

    Plasmid

    #195291

    Purpose

    mCherry reporter containing 13 copies of the p53-binding consensus sequence

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCB-SC101-Spe

    Plasmid

    #133765

    Purpose

    shuttle vector backbone

    Depositor

    Himadri Pakrasi

    Article

    Liu et al Microb Cell Fact. 2018 Mar 26;17(1):48.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector for e.coli and …

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

    Plasmid

    #100835

    Purpose

    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pX330S-2

    Plasmid

    #58778

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1.Gα13-LgB106

    Plasmid

    #134364

    Purpose

    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα13-LgB106 (GNA13 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GPI-mCherry2

    Plasmid

    #182869

    Purpose

    Monomer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    GPI-mCherry2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid

    #107055

    Purpose

    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mCh-alpha-tubulin

    Plasmid

    #49149

    Purpose

    expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNIR-FbmCherry

    Plasmid

    #184676

    Purpose

    NIR-Fb to mCherry

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbmCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLminP_Luc2P_RE9

    Plasmid

    #90343

    Purpose

    HIF1a - Hypoxia gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    HIF1a

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA-SFPQ

    Plasmid

    #166959

    Purpose

    Expresses HA-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEGFP-TDP-43

    Plasmid

    #235487

    Purpose

    Expression of human TDP-43-EGFP

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pHDR-USR-SpCas9

    Plasmid

    #208829

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LaM8-AK74-irFP

    Plasmid

    #159592

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at AK74
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mCherry-Rab5DN(S34N)

    Plasmid

    #35139

    Depositor

    Sergio Grinstein

    Article

    Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Dominant Negative- S34N

    Promoter

    CMV

    Available since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PBCAG-mRFP

    Plasmid

    #40996

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBabepuro-HA-GASC1

    Plasmid

    #24182

    Purpose

    Mammalian expression of human JMJD2C with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    GASC1 (KDM4C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

47. pLentiCRISPR_zeo_sgTP53

    Plasmid

    #183180

    Purpose

    pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgTP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3.1/CALR wt-FLAG

    Plasmid

    #204532

    Purpose

    Mammalian expression of human CALR wt-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR wt-FLAG (CALR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pDONR223_BFP

    Plasmid

    #25891

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    BFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pHRIG-Akt3

    Plasmid

    #53596

    Purpose

    lentiviral expression of constitutively active human Akt3 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    cAkt3 (AKT3 Human)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only