We narrowed to 27,285 results for: Tat

1. pET21d-colEdes4/Imdes4

   Plasmid

   #107112

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes4/Imdes4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21d-colEdes5/Imdes5

   Plasmid

   #107113

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes5/Imdes5
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21d-colEdes6/Imdes6

   Plasmid

   #107114

   Purpose

   plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEdes6/Imdes6
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAW218

   Plasmid

   #113240

   Purpose

   E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

   Depositor

   Jennifer Doudna

   Article

   Wright et al Science. 2017 Sep 15;357(6356):1113-1118

   Inserts

   Cas1
   Cas2
   Leader-repeat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBbS8k-RFP_K102E_E115K

   Plasmid

   #109405

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   K102E and E115K repA mutations to increase copy n…

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBbS8k-RFP_N99K

   Plasmid

   #109409

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   N99K repA mutation to increase copy number

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBbS8k-RFP_K102E

   Plasmid

   #109408

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   K102E repA mutation to increase copy number

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBbS8k-RFP_E115K

   Plasmid

   #109407

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   E115K repA mutation to increase copy number

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBbS8k-RFP_M78I

   Plasmid

   #109413

   Purpose

   RepA Mutant

   Depositor

   Jay Keasling

   Article

   Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

   Insert

   repA
   

   Use

   Tags

   RFP

   Expression

   Bacterial

   Mutation

   M78I repA mutation to increase copy number

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFOSGE_COF3D04

    Plasmid

    #70967

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    etaTry (etaTry Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pfMDH_N300D

    Plasmid

    #67728

    Purpose

    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to aspartate

    Promoter

    T7

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-Ii-OVA.I+II

    Plasmid

    #64596

    Purpose

    Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Invariant chain

    Expression

    Mammalian

    Mutation

    Only amino acids 242-353 (class I and class II ep…

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti_dCas9-KRAB-MeCP2_Blast

    Plasmid

    #221501

    Purpose

    Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    dCas9-KRAB-MeCP2-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P307-FBP_6

    Plasmid

    #139697

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cag.Luc.puro

    Plasmid

    #74409

    Purpose

    Constitutive Luciferase expression in mammalian cells along with puromycin selection

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al EMBO Mol Med. 2017 May;9(5):672-686. doi

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET30a-AlkB-D135S

    Plasmid

    #79051

    Purpose

    Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO-PTEN-shRNA-1320

    Plasmid

    #25638

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-1320 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Ii-OVA.II

    Plasmid

    #64597

    Purpose

    Expression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Invariant chain

    Expression

    Mammalian

    Mutation

    Only amino acids 315-353 (class II epitopes)

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAcGP67A-PSMA

    Plasmid

    #11524

    Depositor

    Pamela Bjorkman

    Article

    Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

    Insert

    prostate-specific membrane antigen (FOLH1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    There is a short N-terminal truncation of this pr…

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG vGlut1 mCherry

    Plasmid

    #105010

    Purpose

    Excitatory presynaptic marker

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    SLC17A7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge