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Addgene

We narrowed to 22,079 results for: his

Showing: 6301 - 6350 of 22079 results
  1. p5A0-T

    Plasmid
    #92016
    Purpose
    Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease
    Depositor
    Inserts
    TALE targeting lac operator
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    J23102 Promoter (from Anderson promoter library) …
    Available since
    Jan. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pEG03

    Plasmid
    #73841
    Purpose
    Encodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.
    Insert
    PhyB(1-917) with C-term his tag (PIL2 Mustard Weed)
    Use
    Dictyostelium discoideum expression
    Tags
    6x his tag
    Expression
    Mutation
    Promoter
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. p5A7-T

    Plasmid
    #92020
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Inserts
    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pPD1178-LPDV promoterless PuroR-P2A-miRFP720

    Plasmid
    #201537
    Purpose
    Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene
    Depositor
    Insert
    Promoterless PuroR-P2A-miRFP720
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pJL1-MBP-DQNAT

    Plasmid
    #198215
    Purpose
    pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Insert
    MBP_DQNAT
    Use
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pJL1-PD-DQNAT

    Plasmid
    #198216
    Purpose
    pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Insert
    PD-DQNAT
    Use
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pJL1-sfGFP-MOORmut

    Plasmid
    #198213
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag
    Depositor
    Insert
    sfGFP_MOORmut
    Use
    Tags
    6x His Tag and mutated (non-permissible) MOORmut …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pET-30a(+)-C-SH2

    Plasmid
    #111419
    Purpose
    express murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)
    Depositor
    Insert
    murine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pET-30a(+)-N-SH2

    Plasmid
    #111274
    Purpose
    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Insert
    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Use
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. WT-eGFP

    Plasmid
    #215168
    Purpose
    Encodes the enhanced green fluorescent protein
    Insert
    WT-eGFP
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pETTEV-SENP8

    Plasmid
    #196361
    Purpose
    protein expression of the His-tagged full length protein
    Depositor
    Insert
    SENP8/NEDP1
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. pCDB24

    Plasmid
    #91959
    Purpose
    empty expression vector for 10His-SUMO tag protein purification
    Depositor
    Type
    Empty backbone
    Use
    Tags
    10-His and Smt3
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pSCBe

    Plasmid
    #110065
    Purpose
    Trc1O promoter express gene in Synechocystis 6803
    Depositor
    Type
    Empty backbone
    Use
    Tags
    3' end His tag and 5' end FLAG tag
    Expression
    Bacterial
    Mutation
    Promoter
    Trc1O
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. MCA2-eGFP

    Plasmid
    #117098
    Purpose
    protein expression in yeast
    Depositor
    Insert
    MCA2-eGFP
    Use
    Tags
    His
    Expression
    Yeast
    Mutation
    Promoter
    AOX
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. MCA1-eGFP

    Plasmid
    #117097
    Purpose
    protein expression in yeast
    Depositor
    Insert
    MCA1-eGFP
    Use
    Tags
    His
    Expression
    Yeast
    Mutation
    Promoter
    AOX
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 6301 - 6350 of 22079 results