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We narrowed to 14,223 results for: Cas9

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Showing: 2521 - 2540 of 14223 results

  1. pAC5-dual-dCas9VP48-sgTetO

    Plasmid
    #48237
    Purpose
    Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9VP48 and sgTetO
    Use
    CRISPR
    Tags
    HA-tag and VP48
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48237-plasmid-map-sequence-id-69544

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  2. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  3. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196712-plasmid-map-sequence-id-388051

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  4. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

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  6. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  9. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid
    #69534
    Purpose
    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.
    Depositor
    Timo Otonkoski
    Publication
    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-dCas9VP192-T2A-EGFP
    Tags
    C-term T2A-EGFP and DHFR Destabilised Domain
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69534-plasmid-map-sequence-id-122831

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  10. PX458-3xHA-SpCas9-HF1

    Plasmid
    #201952
    Purpose
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201952-plasmid-map-sequence-id-405306

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  13. pUC19-A8REH9

    Plasmid
    #103084
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A8REH9(Cas9 coding gene from Eubacterium dolichum DSM 3991)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103084-plasmid-map-sequence-id-209043

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  14. pUC19-K1LM04

    Plasmid
    #103119
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103119-plasmid-map-sequence-id-201919

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  15. pUC19-Q4A5I2

    Plasmid
    #103125
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q4A5I2(Cas9 coding gene from Mycoplasma synoviae (strain 53))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103125-plasmid-map-sequence-id-201888

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  16. pUC19-Q5X8T6

    Plasmid
    #103127
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q5X8T6(Cas9 coding gene from Legionella pneumophila (strain Paris))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103127-plasmid-map-sequence-id-201890

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  17. pUC19-C7G697

    Plasmid
    #103091
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    C7G697(Cas9 coding gene from Roseburia intestinalis L1-82)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103091-plasmid-map-sequence-id-201802

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  18. pUC19-D4J3S7

    Plasmid
    #103096
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    D4J3S7(Cas9 coding gene from Coprococcus catus GD/7)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103096-plasmid-map-sequence-id-201806

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  19. pUC19-E3ELL7

    Plasmid
    #103099
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E3ELL7(Cas9 coding gene from Bifidobacterium bifidum (strain S17))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103099-plasmid-map-sequence-id-201809

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  20. pUC19-F3QMY7

    Plasmid
    #103107
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    F3QMY7(Cas9 coding gene from Parasutterella excrementihominis YIT 11859)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103107-plasmid-map-sequence-id-201816

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Showing: 2521 - 2540 of 14223 results