We narrowed to 67,243 results for: nin

1. p5E TRE-3GV (JDW 1163)

   Plasmid

   #224476

   Purpose

   Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   TRE3GV promoter
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1a-Neuromodulin-IRES-Puromycin

   Plasmid

   #134666

   Purpose

   Expresses EGFP-tagged plasma membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Neuromodulin (GAP43 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. JI401: Ad5 E1A/Hexon qRT-PCR standard

   Plasmid

   #131753

   Purpose

   Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Inserts

   Ad5 E1A gene fragment
   Ad5 Hexon gene fragment
   

   Use

   Standard for qrt-pcr

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pECNUS4

   Plasmid

   #184887

   Purpose

   ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-REPORT(PGK/CMV)-GtwyA

   Plasmid

   #208574

   Purpose

   LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   mnucTomato
   HSVtk
   Neomycin
   d2nucEGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/CMV and minP

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEditA

   Plasmid

   #207531

   Purpose

   Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
   

   Use

   CRISPR; Pseudomonas vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR-SF61

   Plasmid

   #163916

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277

   Insert

   SF5Phe tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-DIO_EGFP-RandE3

   Plasmid

   #79872

   Purpose

   For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. DCT.VV.Orf45

   Plasmid

   #12733

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf45
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

10. 3341 pcDNA3 Bcl-2 T69A S70A S87A

    Plasmid

    #13340

    Depositor

    Stanley Korsmeyer

    Article

    Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Threonine 69 to Alanine; changed Serine 7…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pAAV_tKiMBI-T2A-caMEK

    Plasmid

    #199578

    Purpose

    Expresses tKiMBI and caMEK in an AAV vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
    constitutively active MEK
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. DCT.VV.Orf170

    Plasmid

    #12858

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf170
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

13. AA296

    Plasmid

    #216028

    Purpose

    Fragmid fragment: (Cas protein) NNNNCC PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Nme2Cas9_v1.1 [Nme2]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR P2R-P3-mKate2

    Plasmid

    #48345

    Purpose

    Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    mKate2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a stop codon at the 3' end of the c…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS1871

    Plasmid

    #205976

    Purpose

    U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH DR-BpiI-DR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR P4-P1R-EYFP

    Plasmid

    #48350

    Purpose

    Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EYFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR P2R-P3-ECFP

    Plasmid

    #48353

    Purpose

    Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a stop codon.

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-V5-mScarlet-I_SV40pA (JDW 968)

    Plasmid

    #224529

    Purpose

    A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpeCas9-HF

    Plasmid

    #192141

    Purpose

    Expresses highly specific SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9-HF
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    SpeCas9-HF (R247A, N415A, S421A)

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Tollip M240A,F241A

    Plasmid

    #178051

    Purpose

    GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Methionine 240 to Alanine and changed Phe…

    Promoter

    CMV

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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