8,734 results

1. Rtn4a-GFP

   Plasmid

   #61807

   Purpose

   Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

   Insert

   Rtn4a (RTN4 Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Rtn4a Stop Codon removed

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL2-eNOSPromoter-Luciferase

   Plasmid

   #19297

   Depositor

   Charles Lowenstein

   Article

   Arnet et al J Biol Chem. 1996 Jun 21. 271(25):15069-

   Insert

   endothelial nitric oxide synthase promoter (NOS3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-BiFC-Venus

   Plasmid

   #105804

   Purpose

   Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   VN173 (I152L); VC155

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLXSN16E6E7

   Plasmid

   #52394

   Purpose

   The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

   Depositor

   Denise Galloway

   Article

   Halbert et al J Virol. 1991 Jan;65(1):473-8.

   Insert

   HPV16 E6E7
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-M13-TEV-C-P2A-TdTomato

   Plasmid

   #92391

   Purpose

   produces AAV expressing Cal-light M13-TevC component and TdTomato

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   M13-TEV-C-P2A-TdTomato
   

   Use

   AAV and Synthetic Biology

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. HP4_M13

   Plasmid

   #120340

   Purpose

   Helper plasmid for production of phagemid ssDNA, based on m13mp18

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

   Insert

   Coding part of the M13 phage genome
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   native phage promoters

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMVTNT PINK1 C-myc

   Plasmid

   #13314

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC189

   Plasmid

   #32114

   Depositor

   Eric Rubin

   Article

   Chiang et al Gene. 2002 Aug 21;296(1-2):179-85.

   Type

   Empty backbone

   Use

   Transposon delivery vector

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas13-M3nls

    Plasmid

    #155366

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. DsRed2-Mito-7

    Plasmid

    #55838

    Purpose

    Localization: Mitochondria, Excitation: 558, Emission: 583

    Depositor

    Michael Davidson

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLenti HRE-Luc pGK Hygro

    Plasmid

    #118706

    Purpose

    reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    HRE (HIF-responsive promoter (3xHRE)-luc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MP4

    Plasmid

    #69652

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRL-MHCK7-GCaMP6

    Plasmid

    #65042

    Purpose

    Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

    Depositor

    Nenad Bursac

    Article

    Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

    Insert

    GCaMP6
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    MHCK7

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL2-p21 promoter-Luc

    Plasmid

    #33021

    Depositor

    Martin Walsh

    Article

    Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

    Insert

    p21 promoter (CDKN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    p21

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3993 pET ubcH7

    Plasmid

    #8654

    Depositor

    Peter Howley

    Article

    Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

    Insert

    UbcH7 (UBE2L3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pHR_Gal4UAS_pGK_mCherry

    Plasmid

    #79124

    Purpose

    Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CMV/TO Neo DEST (685-3)

    Plasmid

    #17292

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-MHL

    Plasmid

    #26096

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. paavCAG-post-mGRASP-2A-dTomato

    Plasmid

    #34912

    Purpose

    post

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Has Service

    AAV5

    Insert

    postsynaptic mGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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