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177,170 results

Showing: 6351 - 6400 of 177170 results

  5. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Publication
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Expression
    Mammalian
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    128605-plasmid-map-sequence-id-318004

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  7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    118156-plasmid-map-sequence-id-239644

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  13. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    100903-plasmid-map-sequence-id-196607

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  16. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    91854-plasmid-map-sequence-id-176529

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  17. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Publication
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Expression
    Mammalian
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    87836-plasmid-map-sequence-id-176310

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  18. pcDNA3-hPR-A

    Plasmid
    #89119
    Purpose
    mammalian expression of full-length human progesterone receptor-A, equivalent to human PR-B amino acids 165-933
    Depositor
    Elizabeth Wilson
    Publication
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human progesterone receptor-A (PGR Human)
    Expression
    Mammalian
    Mutation
    human progesterone receptor-A, equivalent to huma…
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    89119-plasmid-map-sequence-id-175729

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  23. pScarlessHD-sfGFP-DsRed

    Plasmid
    #80811
    Purpose
    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    sfGFP-piggyBac-DsRed
    Use
    Unspecified
    Tags
    sfGFP
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    80811-plasmid-map-sequence-id-152800

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  24. Talin-CS

    Plasmid
    #83377
    Purpose
    Control FRET probe for measuring tension on Talin molecule
    Depositor
    Martin Schwartz
    Publication
    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d
    Insert
    Talin1 (Tln1 Mouse)
    Use
    Retroviral
    Tags
    EGFP-F40-tagRFP (tension sensor module)
    Expression
    Mammalian
    Mutation
    Tension sensor module is inserted at C-terminal o…
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    83377-plasmid-map-sequence-id-158762

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  30. pRubiG-T2A-Cas9

    Plasmid
    #75348
    Purpose
    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    GFP (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    75348-plasmid-map-sequence-id-142640

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  38. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid
    #64219
    Purpose
    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA targeting ROSA26-1
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-BFP-P2A-E1B
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    64219-plasmid-map-sequence-id-107960

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  42. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60228
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    60228-plasmid-map-sequence-id-96394

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  43. mCherry-Occludin-N-10

    Plasmid
    #55112
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Occludin (OCLN Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    55112-plasmid-map-sequence-id-257392

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  46. SpyTag-β-Lactamase-SpyCatcher

    Plasmid
    #52656
    Purpose
    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.
    Depositor
    Mark Howarth
    Publication
    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2
    Insert
    TEM-1 β-Lactamase
    Tags
    SpyCatcher and SpyTag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    52656-plasmid-map-sequence-id-422741

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  50. pCAG-hCas9

    Plasmid
    #51142
    Purpose
    Expresses hCas9 under the CAG promoter for CRISPR
    Depositor
    Izuho Hatada
    Publication
    Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hCas9
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
Showing: 6351 - 6400 of 177170 results