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We narrowed to 5,531 results for: PEP

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Showing: 3841 - 3870 of 5531 results

  6. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  9. pRSF_AaRbcLS

    Plasmid
    #229505
    Purpose
    Expresses Anthoceros agrestis Rubisco large and small subunit (C terminus hexahistidine)
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Rubisco large subunit
    Rubisco small subunit
    Tags
    hexahistidine
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229505-plasmid-map-sequence-id-456757

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  18. pcDNA3.1-H2-18 Heavy chain-SpyTag003

    Plasmid
    #216307
    Purpose
    Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216307-plasmid-map-sequence-id-433370

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  21. pcDNA3-FRB-VMAc-CSarcin

    Plasmid
    #70223
    Purpose
    Expresses VMA intein with C-terminal portion of alpha-sarcin
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (C-terminal portion)
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Asp 28 to Cys, deleted amino acids 1-27
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. NSarQ27G-VMAn-FKBP

    Plasmid
    #70230
    Purpose
    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pET30b_OmpAT7Sav

    Plasmid
    #138589
    Purpose
    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d
    Insert
    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    Use
    Synthetic Biology
    Tags
    OmpA export signal and T7 tag
    Expression
    Bacterial
    Promoter
    PT7
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138589-plasmid-map-sequence-id-274488

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  24. pOCC151

    Plasmid
    #118899
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    GST tag, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118899-plasmid-map-sequence-id-232310

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  30. pOCC5

    Plasmid
    #118863
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118863-plasmid-map-sequence-id-232276

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Showing: 3841 - 3870 of 5531 results