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We narrowed to 6,273 results for: nls

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Showing: 5121 - 5160 of 6273 results

  1. pCMV-SpCas9-VRQR(+N1286Q)-P2A-EGFP (LRF247)

    Plasmid
    #242677
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + N1286Q mutations in SpCas9(D1135V/G1218R/N…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242677-plasmid-map-sequence-id-488986

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  2. pCMV-SpCas9-VRQR(+G1104K)-P2A-EGFP (LRF54)

    Plasmid
    #242671
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + G1104K mutations in SpCas9(G1104K/D1135V/G…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242671-plasmid-map-sequence-id-488983

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  3. pCMV-SpCas9-VRQR(+A61R)-P2A-EGFP (KAC1030)

    Plasmid
    #242665
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + A61R mutations in SpCas9(A61R/D1135V/G1218…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242665-plasmid-map-sequence-id-488982

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  4. pCMV-SpCas9-VRQR(+L244Q)-P2A-EGFP (LRF96)

    Plasmid
    #242667
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L244Q mutations in SpCas9(L244Q/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242667-plasmid-map-sequence-id-488981

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  5. pCMV-SpCas9-VRQR(+N1317R)-P2A-EGFP (LRF53)

    Plasmid
    #242678
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + N1317R mutations in SpCas9(D1135V/G1218R/N…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242678-plasmid-map-sequence-id-488980

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  6. pCMV-SpCas9-VRQR(+A1285K)-P2A-EGFP (KAC1134)

    Plasmid
    #242676
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + A1285K mutations in SpCas9(D1135V/G1218R/A…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242676-plasmid-map-sequence-id-488979

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  7. pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49)

    Plasmid
    #242675
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L1245V mutations in SpCas9(D1135V/G1218R/L…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242675-plasmid-map-sequence-id-488976

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  8. pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)

    Plasmid
    #242681
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242681-plasmid-map-sequence-id-488974

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  9. pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

    Plasmid
    #242672
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242672-plasmid-map-sequence-id-488973

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  10. pCMV-SpCas9-VQR-P2A-EGFP (LRF199)

    Plasmid
    #242664
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VQR mutations in SpCas9(D1135V/R1335Q/T1337R)
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242664-plasmid-map-sequence-id-488971

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  11. pCMV-SpCas9-VRQR(+T1138K)-P2A-EGFP (LRF243)

    Plasmid
    #242674
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(T1138K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + T1138K mutations in SpCas9(D1135V/T1138K/G…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242674-plasmid-map-sequence-id-488969

    Enlarge

  12. pCMV-SpCas9-VRQR(+D1332K)-P2A-EGFP (KAC966)

    Plasmid
    #242680
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(D1332K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + D1332K mutations in SpCas9(D1135V/G1218R/D…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242680-plasmid-map-sequence-id-488968

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  15. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

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  16. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  22. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  23. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

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  28. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  31. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71667-plasmid-map-sequence-id-243369

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  33. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid
    #61365
    Purpose
    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    Nm-dCas9-p300 Core (EP300 Neisseria meningiditis, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61365-plasmid-map-sequence-id-244292

    Enlarge

  34. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74407-plasmid-map-sequence-id-140317

    Enlarge

  35. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

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  37. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100936-plasmid-map-sequence-id-206124

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  38. pGfa2(ABDx3)-nLac

    Plasmid
    #53133
    Purpose
    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells
    Depositor
    Michael Brenner
    Article
    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    -2163 to +47, with 3 copies of 3 copies of the gf…
    Promoter
    human Gfa2
    Available Since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53133-plasmid-map-sequence-id-189228

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  40. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    U1a promoter
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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Showing: 5121 - 5160 of 6273 results