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We narrowed to 18,759 results for: nls

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Showing: 5121 - 5160 of 18759 results

  1. pM-CBE

    Plasmid
    #196293
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP
    Depositor
    Zhenghe Li
    Publication
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding CBE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    196293-plasmid-map-sequence-id-389996

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  2. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

    Plasmid
    #188384
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
    Depositor
    Kole Roybal
    Publication
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    Use
    Lentiviral
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188384-plasmid-map-sequence-id-371052

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  6. CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid
    #171380
    Purpose
    Expression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized RfxCas13d
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171380-plasmid-map-sequence-id-337345

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  19. pX330a dCas9-VP64

    Plasmid
    #92363
    Purpose
    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VP64
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92363-plasmid-map-sequence-id-178232

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  26. pX330 Ctnnb1.1

    Plasmid
    #59911
    Purpose
    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter
    Depositor
    Tyler Jacks
    Publication
    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    59911-plasmid-map-sequence-id-123316

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  29. lentiCRISPR - EGFP sgRNA 5

    Plasmid
    #51764
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    Use
    CRISPR and Lentiviral
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51764-plasmid-map-sequence-id-79386

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  30. pAC84-pCR8-dCas9

    Plasmid
    #48218
    Purpose
    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9(D10A;H840A)
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag
    Mutation
    D10A;H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48218-plasmid-map-sequence-id-69495

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  37. TDP-43 mRuby K84ONBK

    Plasmid
    #216226
    Purpose
    TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.
    Depositor
    Jennifer Lee
    Publication
    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do
    Insert
    Transactive response DNA binding protein of 43 kDa (TARDBP Human)
    Expression
    Mammalian
    Mutation
    Lysine 84 mutated to a TAG stop codon
    Promoter
    CMV Promoter
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    216226-plasmid-map-sequence-id-431056

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  38. pSH-Csy4-T2A-SaRFN

    Plasmid
    #85755
    Purpose
    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-T2A-FokI-SadCas9
    Use
    CRISPR
    Tags
    3xHA tag, Csy4, and FokI fusion via 36 amino acid…
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85755-plasmid-map-sequence-id-170274

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  40. PE7_AAVS1_knockin_HDR_donor

    Plasmid
    #223001
    Purpose
    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7
    Use
    Hdr donor
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    EF1α
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    223001-plasmid-map-sequence-id-442579

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Showing: 5121 - 5160 of 18759 results