We narrowed to 8,515 results for: SET

1. psiTE(2)-Gal4

   Plasmid

   #220451

   Purpose

   Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. psiTE(0)-Gal4DBD

   Plasmid

   #220452

   Purpose

   Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4DBD-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. psiTE(2)-Gal4DBD

   Plasmid

   #220454

   Purpose

   Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4DBD-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psiTE(1)-p65AD

   Plasmid

   #220456

   Purpose

   Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-p65AD-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. psiTE(0)-Gal4

   Plasmid

   #220449

   Purpose

   Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. psiTE(2)-LexAGAD

   Plasmid

   #220460

   Purpose

   Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-LexAGAD-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. psiTE(1)-Gal4DBD

   Plasmid

   #220453

   Purpose

   Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-Gal4DBD-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. psiTE(1)-LexAGAD

   Plasmid

   #220459

   Purpose

   Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-LexAGAD-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiTE(0)-p65AD

   Plasmid

   #220455

   Purpose

   Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

   Depositor

   Benjamin White

   Article

   Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   CfaN-p65AD-CfaC
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psiTE(1)-Gal4

    Plasmid

    #220450

    Purpose

    Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-Gal4-CfaC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psiTE(0)-LexAGAD

    Plasmid

    #220458

    Purpose

    Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-LexAGAD-CfaC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psiTE(2)-p65AD

    Plasmid

    #220457

    Purpose

    Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

    Depositor

    Benjamin White

    Article

    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    CfaN-p65AD-CfaC
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSGKP_AcsgRNA

    Plasmid

    #203807

    Purpose

    pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOCC154

    Plasmid

    #118902

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBDK

    Plasmid

    #135190

    Purpose

    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    KanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cat

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMH3

    Plasmid

    #52528

    Purpose

    serves as PCR-template to generate HR donors for C-terminal GFP-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    eGFP and Blasticigin resistance cassette
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOCC153

    Plasmid

    #118901

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Plasmid

    #58440

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMVV098

    Plasmid

    #215304

    Purpose

    Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PPhIF

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSC6

    Plasmid

    #104780

    Purpose

    pSC218UG-At Ubiquitin10:Gateway expression cassette binary vector

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJW1354

    Plasmid

    #69490

    Purpose

    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

    Depositor

    Jordan Ward

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    

    Use

    CRISPR; Template to generate cassettes for -media…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSC5

    Plasmid

    #104779

    Purpose

    pSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vector

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pADH147

    Plasmid

    #90991

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Plasmid

    #58439

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHSP70B-CP6-bsr

    Plasmid

    #165878

    Purpose

    Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pADH119

    Plasmid

    #90985

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCORE-Kp53

    Plasmid

    #72241

    Purpose

    Contains the CORE cassette with KanMX4 and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    kanMX4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGolden-Hyg

    Plasmid

    #51423

    Purpose

    Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    PGK-Hyg-pA (PGK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAEF5

    Plasmid

    #136305

    Purpose

    Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

    Depositor

    Gilles Fischer

    Article

    Agier et al Methods Mol Biol. 2021;2196:181-198. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAH237

    Plasmid

    #121146

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. IQ013: pMVP/Ad-DEST

    Plasmid

    #121843

    Purpose

    pMVP destination vector, empty adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMA-SpCas9-g10

    Plasmid

    #80793

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMTIKA

    Plasmid

    #22061

    Depositor

    Maria Castro

    Article

    Xiong et al J Virol. 2006 Jan . 80(1):27-37.

    Insert

    mCMV-TetOn expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

36. MTK8b_004

    Plasmid

    #123892

    Purpose

    Encodes the 3rd gen. 5' HIV LTR cassette as a type 8b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    5' HIV LTR (3rd gen.)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV GG hUbV-EGFP

    Plasmid

    #216161

    Purpose

    Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTAJAK-71 (pESC-NatMXsyn-USER)

    Plasmid

    #78232

    Purpose

    Empty vector, for the insertion of gRNA expression cassettes into the vector.

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR2B-dCas9-KRAB

    Plasmid

    #115547

    Purpose

    Entry cassette for dCas9-KRAB

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Insert

    dCas9-KRAB
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMVV104

    Plasmid

    #215310

    Purpose

    Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PAraBAD

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBS AluYa5rescueA70D

    Plasmid

    #78657

    Purpose

    Generates Alu inserts suitable for rescue

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

    Insert

    Ya5rescue cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. MTK7_014

    Plasmid

    #123881

    Purpose

    Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    3' HIV LTR (3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSCL.028

    Plasmid

    #184965

    Purpose

    Test effect of extending -Eco1 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDYSCKO-ADE1

    Plasmid

    #120264

    Purpose

    pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    pDYSCKO cassette-ADE1 targeting guide
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    SNR52

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAC1750

    Plasmid

    #127984

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAL119

    Plasmid

    #21909

    Depositor

    Maria Castro

    Article

    Ali et al Mol Ther. 2004 Dec . 10(6):1071-84.

    Insert

    hCMV promoter expression cassette
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

47. pgRNA-backbone

    Plasmid

    #172517

    Purpose

    Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

    Depositor

    Leonid Kruglyak

    Article

    Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

    Insert

    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. MXS_PGK::tTA2-bGHpA

    Plasmid

    #62448

    Purpose

    MXS_chaining vector with PGK::tTA2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tetracycline transactivator with PGK promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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49. pUF-AsCpf1-pre-gRNA

    Plasmid

    #137849

    Purpose

    AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    AsCpf1 and pre-gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. PB-EEA-g1-PGK-Puro

    Plasmid

    #102908

    Purpose

    PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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