We narrowed to 4,229 results for: Mcc
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Plasmid#200022PurposeFor lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoterDepositorInsert3xHA-TurboID-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GsAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F263-OKT3
Plasmid#190680PurposeEncodes an EGFRvIII-CD3 BITE protein (F263 clone)DepositorInsertAnti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F269-OKT3
Plasmid#190681PurposeEncodes an EGFRvIII-CD3 BITE protein (F269 clone)DepositorInsertAnti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHrD-IRES-Fluc
Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS ā¦Available SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CRISPR_DpmrA
Plasmid#160903PurposeCRISPR_Cas9, lambda red recombineeringDepositorInsertsCas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette
UseTagsExpressionMutationPromoteraraBADAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT.000
Plasmid#119559PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-RFP657
Plasmid#75162PurposeDerived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsRFP657ExpressionMammalianMutationPromoterAvailable SinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F265-OKT3
Plasmid#190679PurposeEncodes an EGFRvIII-CD3 BITE protein (F265 clone)DepositorInsertAnti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO ALIX Bro1-V (1-702)
Plasmid#80642PurposeResidues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648DepositorInsertALIX Bro1-V (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-702PromoterAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F271-OKT3
Plasmid#190682PurposeEncodes an EGFRvIII-CD3 BITE protein (F271 clone)DepositorInsertAnti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEFSAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
UseTagsExpressionYeastMutationPromoterAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-mB2M
Plasmid#154091PurposeSelf-Cutting and Integrating CRISPR backbone targeting murine B2MDepositorInsertsgRNA and BAIT targeting murine B2M locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 D2335A/N2340K
Plasmid#199575PurposeExpresses FLAG-tagged kinase dead SMG1DepositorAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL-IRES-ST-mCherry
Plasmid#110727PurposeExpresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherryDepositorInsertsUseTagsStr Streptavidin binding peptide (SBP) and mCherrā¦ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMV and IRESAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev
Plasmid#101340PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.DepositorInsertGFP, tat, rev
UseLentiviralTagsExpressionMutationPromoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Īrev
Plasmid#101348PurposeAll mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta revPromoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (pGEX2T(TEV))
Plasmid#80630PurposeBacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197DepositorAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG SMG1 T3550A/S3556A
Plasmid#199574PurposeExpresses FLAG-tagged mutated SMG1DepositorAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM0638
Plasmid#129005PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC101 NL4-3 envFS Īrev ĪRRE RTEm26CTE
Plasmid#101351PurposeHIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationPromoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0641
Plasmid#129006PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter AP-1 site mutant pGL3Basic
Plasmid#32728DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferase; Luciferase reporter geneTagsExpressionMutation-947 AP-1 binding site TGAGTCA deletionPromoter-962 CCND1 promoterAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQCi-hTRAC-sgRNA
Plasmid#154093PurposepQCi backbone with sgRNA targeting human TCR-alpha common chainDepositorInsertsgRNA targeting human TRAC locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter CREB site mutant pGL3Basic
Plasmid#32732DepositorInsertCCND1 Promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-51 CREB site TAACGTCA deletionPromoter-962 CCND1 PromoterAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pet16b ALIX Bro1 (1-359)
Plasmid#80641PurposeResidues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428DepositorInsertALIX Bro1, residues 1-359 (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-359PromoterT7Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCIPpay-BFP reporter
Plasmid#154096PurposeSelf-Cutting and Integrating Payload CRISPR backbone with BFP reporterDepositorInsertP2A-tagBFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic
Plasmid#32733DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) site CTTTGAT deletionPromoter-962 CCND1Available SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry2-sgC9cen
Plasmid#198326PurposeExpresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporterDepositorInsertChr9-CEN guide RNA
UseTagsExpressionMammalianMutationPromoterhU6Available SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsA site mutant pGL3Basic
Plasmid#32729DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferase; Luciferase reporter geneTagsExpressionMutation-822 EtsA site AGGAA deletionPromoter-962 CCND1 promoterAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsB site mutant pGL3Basic
Plasmid#32730DepositorInsertCCND1 (CCND1 Human)
UseLuciferaseTagsExpressionMutation-778 EtsB binding site AGGAA deletionPromoter-962 CCND1Available SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsC site mutant pGL3Basic
Plasmid#32731DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-424 EtsC site TTCCT deletionPromoter-962 CCND1 promoterAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-hTRAC
Plasmid#154094PurposeSelf-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chainDepositorInsertsgRNA and BAIT targeting human TRAC locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic
Plasmid#32734DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deletiā¦Promoter-962 CCND1Available SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMM0647
Plasmid#129008PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
UseTagsExpressionYeastMutationPromoterAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic
Plasmid#32735DepositorInsertCCND1 Promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletionā¦Promoter-962 CCND1Available SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_ORF18_TurboID_3xHA
Plasmid#200017PurposeExpresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHADepositorInsertORF18
UseTagsTurboID-3xHAExpressionMammalianMutationPromoterCMVAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_ORF30_TurboID_3xHA
Plasmid#200018PurposeExpresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHADepositorInsertORF30
UseTagsTurboID-3xHAExpressionMammalianMutationPromoterCMVAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_ORF31_TurboID_3xHA
Plasmid#200019PurposeExpresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHADepositorInsertORF31
UseTagsTurboID-3xHAExpressionMammalianMutationPromoterCMVAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_ORF66_TurboID_3xHA
Plasmid#200020PurposeExpresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHADepositorInsertORF66
UseTagsTurboID-3xHAExpressionMammalianMutationPromoterCMVAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO_3xHA_TurboID_ORF34
Plasmid#200021PurposeExpresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboIDDepositorInsertORF34
UseTags3xHA-TurboIDExpressionMammalianMutationPromoterCMV promoterAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only