We narrowed to 44,723 results for: ina

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pET24a-VHH-mCardinal2

Plasmid

#243766

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCardinal2. VHH-mCardinal2 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mCardinal2-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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REV7 C-terminal sgRNA

Plasmid

#207095

Purpose

pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GCTCATAAAGGCAGCTGAGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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N-Terminal Halo XRCC4

Plasmid

#207541

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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N-terminal Halo-Ku70

Plasmid

#207547

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with flanked by human Ku70 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactiveEmpty

Plasmid

#213168

Purpose

Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 N-terminal sgRNA

Plasmid

#207094

Purpose

pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTACTGCAGAATGACTACAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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NBS1 C-terminal sgRNA

Plasmid

#207092

Purpose

pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TAAAAAGGAGAAGATAACTG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD1 N-terminal sgRNA

Plasmid

#207101

Purpose

pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATGGCAGGACTATGGCAGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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ATM C-terminal sgRNA

Plasmid

#207097

Purpose

pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTCTAAAGGCTGAATGAAA

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mCardinal

Plasmid

#197258

Purpose

Expresses the protein mCardinal in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Mss4-Kina

Plasmid

#202739

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:S. cerevisiae Mss4 (377-756) (MSS4 Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4D636K-Kina

Plasmid

#202725

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)

Expression

Mammalian

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4-Kina

Plasmid

#202724

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756) (MSS4 Budding Yeast)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Mss4D636K-Kina

Plasmid

#202740

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:S. cerevisiae Mss4 (377-756)(D636K) (MSS4 Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-StefinA

Plasmid

#182418

Purpose

Bacterial expression of mNeongreen-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-stefin (CSTA Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-NematocilinA

Plasmid

#193010

Purpose

Plasmid to generate an ISH probe for Hydra Nematocilin A

Depositor

Brigitte Galliot

Article

Vogg et al bioRxiv 2021.12.22.47383

Insert

Hydra Nematocilin A

Use

Cloning vector

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-NLS

Plasmid

#184450

Purpose

Nucleus-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-3xNLS

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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