We narrowed to 29,679 results for: REP

1. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

   Plasmid

   #162164

   Purpose

   Selectable homology donor template for Aag2 cell AGO1

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   RFP
   3xFLAG
   

   Use

   CRISPR and Cre/Lox; Homology donor template

   Tags

   AGO1 homology and loxP

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and endogenous AGO1

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p15_attB(bxb)_lox

   Plasmid

   #183764

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC8_MF (VE5596)

   Plasmid

   #139768

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   PH or p10

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LT3GECIR

   Plasmid

   #111178

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   T3G

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti CMV TRE3G BCL2L1 Neo

   Plasmid

   #140749

   Purpose

   Inducibly expresses Bcl-xL in mammalian cells

   Depositor

   Scott Dixon

   Article

   Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TRE3G

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GLuc-FLAG-CB1

   Plasmid

   #158066

   Purpose

   Membrane expression detection of Cannabinoid receptor 1

   Depositor

   Adolfo Rivero-Muller

   Article

   Rodriguez-Rodriguez et al Sci Rep. 2020 Oct 23;10(1):18191. doi: 1

   Insert

   CB1 (CNR1 Human)
   

   Use

   AAV

   Tags

   Gaussia Luciferase

   Expression

   Mammalian

   Mutation

   contains 4Kb of 3' UTR

   Promoter

   pCAG

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

   Plasmid

   #182390

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-mNeongreen-His
   OmpA-mCherry
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET151-GyrB

   Plasmid

   #216233

   Purpose

   S. aureus USA300 GyrB Codon-optimized for E. coli

   Depositor

   Eric Brown

   Article

   French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

   Insert

   DNA gyrase
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LT3REVIR

   Plasmid

   #111176

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. hDAGLa-V5

    Plasmid

    #87676

    Purpose

    Expresses human DAGL? protein with intracellular V5 tag located on the C-terminal

    Depositor

    Pat Doherty

    Article

    Singh et al Biosci Rep. 2016 May 6;36(3). pii: e0033

    Insert

    Human Diacylglycerol Lipase Alpha (DAGLA Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGH00.0126

    Plasmid

    #64257

    Purpose

    Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation

    Depositor

    Siela Maximova, Mark Guiltinan

    Article

    Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E

    Inserts

    EGFP
    neomycin phosphotransferase II
    

    Use

    Binary agrobacterium vector for plant transformat…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    E12-omega

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xFLAG-CUL2-pCMV7.1

    Plasmid

    #155020

    Purpose

    N-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 2 (CUL2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1+(Zeo)-FLAG-muGFP

    Plasmid

    #166726

    Purpose

    Expresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cells

    Depositor

    Daniel Scott

    Article

    Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

    Insert

    muGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXJ-Myc-KLF2

    Plasmid

    #232654

    Purpose

    transient expresses KLF2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    KLF2 (KLF2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3xFLAG-CUL5-pCMV7.1

    Plasmid

    #155024

    Purpose

    N-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 5 (CUL5 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

    Plasmid

    #184632

    Purpose

    Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad67 (Gad1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2xCBX7CD_W35A-IFP2.0C

    Plasmid

    #215755

    Purpose

    Expresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xCBX7CD_W35A-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LEPZ

    Plasmid

    #111161

    Purpose

    miR-E (miR-30 variant)-based RNAi with ZsGreen reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-MLC2 WT

    Plasmid

    #195235

    Purpose

    Mammalian expression of GFP tagged MLC2. Wild-type

    Depositor

    Keith Mostov, David Bryant

    Article

    Yu et al EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1

    Insert

    MLC2 (MYL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST40-2XFL-Src-Y530F

    Plasmid

    #140319

    Purpose

    Expresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Y530F

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

    Plasmid

    #182415

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCDNA3_V5-APEX2-HMOX1

    Plasmid

    #85824

    Purpose

    Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    V5-APEX2-HMOX1 (HMOX1 Synthetic, Human)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #162334

    Purpose

    Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSaCas9-KRAB
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85038

    Purpose

    For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    FRT-stop-FRT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-CAG-Electra2-P2A-EGFP

    Plasmid

    #184936

    Purpose

    Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pXJ-His-VTCN1

    Plasmid

    #232664

    Purpose

    transient expresses VTCN1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    VTCN1 (VTCN1 Human)
    

    Use

    Tags

    His and His (retained after signal peptide cleava…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLv-EF1a-Parkin-A92mKO2

    Plasmid

    #213559

    Purpose

    Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

    Depositor

    Lani Wu, Steven Altschuler

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSCMV-H2B-FT-Medium

    Plasmid

    #157670

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Medium-FT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

    Plasmid

    #85041

    Purpose

    This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    loxP-stop-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. FgH1tUTG_mup53_Ex5

    Plasmid

    #85535

    Purpose

    Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    mu p53
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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