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Showing: 6451 - 6500 of 6616 results

  5. RCAS-sgATG7

    Plasmid
    #75228
    Purpose
    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh
    Depositor
    Noor Gammoh
    Article
    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:
    Insert
    Autophagy Related 7 (Atg7 Mouse)
    Use
    Retroviral
    Promoter
    U6
    Available Since
    June 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. OPRL1-DuET

    Plasmid
    #213360
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRL1 (OPRL1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213360-plasmid-map-sequence-id-430882

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  9. GNRHR-DuET

    Plasmid
    #213254
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GNRHR (GNRHR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213254-plasmid-map-sequence-id-430804

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  11. pcDNA3.1 NKX6-2(1-196)-Venus

    Plasmid
    #209182
    Purpose
    Mammalian expression of human NKX6-2 cDNA SPAX8-related truncated mutation containing the amino acids 1 to 196; fused with the green fluorescent protein Venus allowing the use of fluorescent based protocols.
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-196) fused with Venus (NKX6-2 Human)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209182-plasmid-map-sequence-id-413578

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  13. pcDNA3.1 NKX6-2(1-188)-Venus

    Plasmid
    #203714
    Purpose
    SPAX8-related truncated mutation (E189*) containing the amino acids 1 to 188 of human NKX6-2 fused with the Venus fluorescent protein
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-188) fused with Venus (NKX6-2 Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    deletion of amino acids 189-277
    Promoter
    CMV
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203714-plasmid-map-sequence-id-401198

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  14. pcDNA3.1 NKX6-2(1-40)-Venus

    Plasmid
    #203713
    Purpose
    SPAX8-related truncated mutation (K41*) containing the amino acids 1 to 40 of human NKX6-2, fused with the Venus fluorescent protein.
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-40) fused with Venus (NKX6-2 Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    deletion of amino acids 41-277
    Promoter
    CMV
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203713-plasmid-map-sequence-id-401045

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  15. pCSmChnERT(#99)

    Plasmid
    #184063
    Purpose
    cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d
    Insert
    mCherry-nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184063-plasmid-map-sequence-id-370311

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  16. pTS2329

    Plasmid
    #169629
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169629-plasmid-map-sequence-id-366778

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  17. pTS2320

    Plasmid
    #169625
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169625-plasmid-map-sequence-id-365625

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  19. vPyACR_2164382

    Plasmid
    #153029
    Purpose
    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
    Depositor
    Peter Hegemann
    Article
    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.
    Insert
    vPyACR_2164382
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    vPyACR_2164382 was C-terminally truncated to incl…
    Promoter
    CMV (+ enhancer)
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    153029-plasmid-map-sequence-id-300511

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  20. vPyACR_21821

    Plasmid
    #153030
    Purpose
    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
    Depositor
    Peter Hegemann
    Article
    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.
    Insert
    vPyACR_21821
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    vPyACR_21821 was C-terminally truncated to includ…
    Promoter
    CMV (+ enhancer)
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    153030-plasmid-map-sequence-id-300513

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  33. CRHR2-DuET

    Plasmid
    #213216
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CRHR2 (CRHR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213216-plasmid-map-sequence-id-430778

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  43. AP2u2-CMV-S_protein

    Plasmid
    #226456
    Purpose
    Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Insert
    Spike Protein
    Tags
    6xHis Tag, Foldon Domain, and Thrombin Cleavage S…
    Expression
    Mammalian
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226456-plasmid-map-sequence-id-458388

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  47. GRPR-DuET

    Plasmid
    #213306
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRPR (GRPR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213306-plasmid-map-sequence-id-430844

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Showing: 6451 - 6500 of 6616 results