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We narrowed to 31,311 results for: uros

Showing: 6451 - 6500 of 31311 results

  10. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99374-plasmid-map-sequence-id-430620

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  12. eMscL WT

    Plasmid
    #107454
    Purpose
    eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Promoter
    human synapsin 1
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107454-plasmid-map-sequence-id-207518

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  17. lentiGuide-Hygro-dTomato

    Plasmid
    #99376
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99376-plasmid-map-sequence-id-191027

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  22. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100903-plasmid-map-sequence-id-196607

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  23. TFORF3414

    Plasmid
    #144890
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NEUROG1 (NEUROG1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144890-plasmid-map-sequence-id-404478

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  24. lentiGuide-Hygro-eGFP

    Plasmid
    #99375
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99375-plasmid-map-sequence-id-191025

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  25. p5E-elavl3

    Plasmid
    #75025
    Purpose
    Can be used to drive pan neuronal expression in zebrafish.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    elavl3
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75025-plasmid-map-sequence-id-141916

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  28. TFORF2945

    Plasmid
    #144421
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NEUROD1 (NEUROD1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    144421-plasmid-map-sequence-id-386493

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  29. lentiGuide-Hygro-iRFP670

    Plasmid
    #99377
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99377-plasmid-map-sequence-id-191028

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  30. pAAV-hSyn-GRAB_NE1m

    Plasmid
    #123308
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE sensor GRAB_NE1m
    Use
    AAV
    Mutation
    contains a glycine-to-threonine mutation at posit…
    Promoter
    hSyn
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123308-plasmid-map-sequence-id-240666

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  39. pMIA10.7

    Plasmid
    #214451
    Purpose
    BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.
    Depositor
    Roger Foo
    Article
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Inserts
    Tet-On 3G
    PuroR
    TRE3G CopGFP
    Use
    Bxbi donor
    Tags
    PuroR, T2A, and Tet-On 3G
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214451-plasmid-map-sequence-id-431689

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  46. CaV1.3e[8a,11,31b,Δ32,Δ42a]

    Plasmid
    #49332
    Purpose
    Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Diane Lipscombe
    Article
    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.
    Insert
    CACNA1D (Cacna1d Rat)
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…
    Promoter
    CMV
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    49332-plasmid-map-sequence-id-215242

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Showing: 6451 - 6500 of 31311 results