We narrowed to 29,679 results for: REP

1. pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

   Plasmid

   #62956

   Purpose

   Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR221-TCF7

   Plasmid

   #79498

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   TCF7 (TCF7 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBR_attB(C31)_FRT

   Plasmid

   #183761

   Purpose

   phiC31-specific donor plasmid for the cloning of payloads <20 kb

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER-ABKAR

   Plasmid

   #61508

   Purpose

   Expression of AMPK biosensor targeted to the endoplasmic reticulum

   Depositor

   Takanari Inoue, Jin Zhang

   Article

   Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

   Insert

   AMPK Biosensor
   

   Use

   Tags

   ER targeting sequence from CYP450 2C1 (see commen…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

   Plasmid

   #184631

   Purpose

   Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gad65 (Gad2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PGK EGFP-PPM1H-WT

   Plasmid

   #200084

   Purpose

   Mammalian expression of wild-type PPM1H

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   PPM1H (PPM1H Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-1_VNp-mNeongreen_CydAB-mCherry

   Plasmid

   #182391

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-mNeongreen-His
   cydAB-mCherry
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cox7c-pHLuorin-sems

   Plasmid

   #107766

   Purpose

   labeling of mitochondrial cytochrome c oxidase, pH measurements

   Depositor

   Karin Busch

   Article

   Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

   Insert

   Subunit Cox7c fused to pHluorin (COX7C Human)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. myc-hIFITM3-delta59-68

   Plasmid

   #104361

   Purpose

   Expresses human IFITM3-delta59-68 with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

   Insert

   IFITM3 (IFITM3 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   deleted amino acids 59 through 68

   Promoter

   CMV

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LEPG

    Plasmid

    #111160

    Purpose

    miR-E (miR-30 variant)-based RNAi with GFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET32a-hEPR

    Plasmid

    #199233

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Proepiregulin preproprotein (EREG Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

    Plasmid

    #62954

    Purpose

    Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

    Plasmid

    #62955

    Purpose

    Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PM-ABKAR

    Plasmid

    #61506

    Purpose

    Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

    Depositor

    Takanari Inoue, Jin Zhang

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    Lyn kinase sequence (see comments)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pAM-DCA-muGFP

    Plasmid

    #166727

    Purpose

    AAV, encodes monomeric ultrastable GFP (muGFP)

    Depositor

    Daniel Scott

    Article

    Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

    Insert

    muGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Chicken Beta Actin

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCMV-H2B-FT-Fast

    Plasmid

    #157669

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Fast-FT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE

    Plasmid

    #67291

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Asc1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSCMV-H2B-FT-Slow

    Plasmid

    #157671

    Purpose

    Retroviral vector for expressing chimeric human histone H2B fused with the "Slow" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.

    Depositor

    Shangqin Guo

    Article

    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi

    Insert

    Histone H2B
    

    Use

    Retroviral

    Tags

    Slow-FT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Donor-PIGA

    Plasmid

    #153530

    Purpose

    Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    PIGA (a 1,991-bp fragment from intron 5 and exon 6)
    

    Use

    Donor plasmid

    Tags

    N/A

    Expression

    Mutation

    No mutation

    Promoter

    No promoter

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. psigma1-EGFP

    Plasmid

    #53611

    Purpose

    Expresses sigma1 of adaptor protein of AP1

    Depositor

    Tom Kirchhausen

    Article

    Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

    Insert

    sigma 1 (Ap1s1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBABR IRFP-Bcd-LEXY

    Plasmid

    #182595

    Purpose

    Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Use

    Tags

    IRFP and LEXY

    Expression

    Insect

    Mutation

    Promoter

    maternal tubulin

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBS-KS-attB2-SA(0)-T2A-Gal4

    Plasmid

    #62899

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 2xHP1bCD_W42A-IFP2.0C

    Plasmid

    #215751

    Purpose

    Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xHP1bCD_W42A-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PXL

    Plasmid

    #75349

    Purpose

    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCX-SpiCee-Kras

    Plasmid

    #140838

    Purpose

    mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-Kras
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FgH1tUTG_mup53_Ex4

    Plasmid

    #85534

    Purpose

    Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    mu p53
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hsp70: mKate2-DN Kif5a

    Plasmid

    #105972

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Kif5a (kif5aa Zebrafish)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    truncation of aa416-1033

    Promoter

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lenti-eCas9-sgNT

    Plasmid

    #140238

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PGK ARF6(Q67L)-CFP

    Plasmid

    #200428

    Purpose

    Mammalian expression of Q67L mutation in ARF6

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    PGK

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA3_HMOX1-V5-APEX2

    Plasmid

    #85823

    Purpose

    Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    HMOX1-V5-APEX2 (HMOX1 Human, Synthetic)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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