We narrowed to 16,938 results for: She

1. pcDNA3 H2B-mIFP T2A Mpro (C145A)

   Plasmid

   #163080

   Purpose

   Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

   Depositor

   Xiaokun Shu

   Insert

   Inactive Mpro mutant C145A and H2B-mIFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

   Plasmid

   #180156

   Purpose

   tdTomato cre reporter with Hipp11 recombineering homology arms

   Depositor

   Mario Capecchi

   Insert

   tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pE-SUMO CRYBB2

   Plasmid

   #80749

   Purpose

   Expression of human beta B2-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYBB2 (CRYBB2 Human)
   

   Use

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BDD FVIII H4

   Plasmid

   #46774

   Purpose

   pcDNA4.BDD FVIII.H4(R484H)

   Depositor

   Robert Peters

   Insert

   B-domain deleted FVIII H4 (R484H) (F8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R484H

   Promoter

   CMV

   Available since

   Nov. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

   Plasmid

   #110547

   Purpose

   CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.

   Depositor

   Ichiro Matsumura

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pBAD

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcBRCA1-385

   Plasmid

   #61586

   Purpose

   Expression of BRCA1 in mammalian cells

   Depositor

   Lawrence Brody

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. BDD FVIII H3

   Plasmid

   #46775

   Purpose

   pcDNA4.BDD FVIII.H3(M2238V)

   Depositor

   Robert Peters

   Insert

   B-domain deleted FVIII H3 protein (M2238V) (F8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M2238V

   Promoter

   CMV

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KSP_K560GFP

   Plasmid

   #129770

   Purpose

   Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged

   Depositor

   William Hancock

   Insert

   KSP (kif11.L Frog)
   

   Use

   Tags

   GFP and His6

   Expression

   Bacterial

   Mutation

   Truncation at 367, fused to kinesin-1 starting Al…

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TBC1D7

   Plasmid

   #32047

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TBC1D7 (TBC1D7 Human)
   

   Use

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. isoform PKC Zeta 1 (JCE875)

    Plasmid

    #182027

    Purpose

    yeast expression of mouse PKC Zeta isoform 1 with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Insert

    PKC Zeta isoform 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SETD2

    Plasmid

    #40741

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETD2 (SETD2 Human)
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

12. XBB.1.5+L455F+F456L

    Plasmid

    #208588

    Purpose

    Mammalian expression vector for expressing XBB.1.5+L455F+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+L455F+F456L spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PTPN22

    Plasmid

    #38933

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    G126V, V147G

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCDZ2FE_Rev3_1873-2025_4PA

    Plasmid

    #105673

    Purpose

    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein with mutations abolishing Rev7 binding

    Depositor

    Thanos Halazonetis

    Insert

    Rev3L (REV3L Human)
    

    Use

    Tags

    2X Flag tag

    Expression

    Mammalian

    Mutation

    Mutations: P1880A, P1885A, P1996A, P2001A

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHRW32

    Plasmid

    #130972

    Purpose

    HyPr plasmid with ble marker

    Depositor

    Chris Todd Hittinger

    Article

    Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

    Insert

    empty backbone
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV hSyn-Flex RG-cerulean

    Plasmid

    #98221

    Purpose

    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus

    Depositor

    Troy Margrie

    Insert

    Cerulean-E2A-RG
    

    Use

    AAV

    Tags

    cerulean

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCHA_OROV_NSs

    Plasmid

    #208939

    Purpose

    Expresses C-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    NSs
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 66 bp from 5' end and deleted 415 b…

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HaloTag-G protein gamma-5

    Plasmid

    #67039

    Purpose

    G protein gamma-5 tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-G protein gamma-5 (GNG5 Bovine)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His6x-EX-HA-FRB-MBD_pYFJ16

    Plasmid

    #120917

    Purpose

    Expresses His6-EX-HA-FRB-MBD in bacteria for protein purification

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    His6-EX-HA-FRB-MBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mmu-miR-30a-5p Block-iT design (BLK)

    Plasmid

    #107789

    Purpose

    miR-30a RNAi Expression

    Depositor

    Fabio Penna

    Insert

    Mmu-miR-30a-5p mature sequence (Mir30a Mouse)
    

    Use

    RNAi

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTRY4_HuC

    Plasmid

    #65753

    Purpose

    entry vector with HuC

    Depositor

    Thomas Tuschl

    Insert

    ELAVL3 (ELAVL3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEMB52/Bag5_1_447

    Plasmid

    #40540

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Bag5 (BAG5 Human)
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Mutation

    WT; aa1-447

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. RPRD1B

    Plasmid

    #40739

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RPRD1B (RPRD1B Human)
    

    Use

    Tags

    6xHis TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

24. pAAVS hOPM

    Plasmid

    #112498

    Purpose

    Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in AAVS locus.

    Depositor

    Bruce Conklin

    Insert

    OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

    Plasmid

    #68337

    Purpose

    Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

    Depositor

    Feng Zhang

    Insert

    St3Cas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-mGAT1

    Plasmid

    #22804

    Depositor

    Henry Lester

    Article

    Mager et al J Neurosci. 1996 Sep 1. 16(17):5405-14.

    Insert

    pcDNA3.1-mGAT1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Jan. 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUASp YFP Ras64B WT

    Plasmid

    #52245

    Purpose

    Drosophila expression of YFP-Ras64B

    Depositor

    Matthew Scott

    Insert

    Ras64B (Ras64B Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

28. PANK1

    Plasmid

    #32871

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PANK1 (PANK1 Human)
    

    Use

    Tags

    His tag (with Thrombin cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 231-597 only

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

29. pCMV-T7-ABEmax-SpG

    Plasmid

    #195278

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP

    Depositor

    Carsten Lederer, Petros Patsali

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV Agpat2-V5

    Plasmid

    #175159

    Purpose

    Lentiviral expression of mouse Agpat2-V5

    Depositor

    Larry Gerace

    Insert

    Agpat2 (Agpat2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV-Enh eGFP Reporter-huKC2-IGR21

    Plasmid

    #59456

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the human keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    huKrt1 3’intergenic region
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Mu Hsp68 minimal promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pUltra-hot-H2B-mTagBFP2

    Plasmid

    #161792

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Insert

    H2B-mTagBFP2
    

    Use

    Lentiviral; Multicistronic

    Tags

    mCherry and mTagBFP2

    Expression

    Mammalian

    Mutation

    I174A in the mTagBFP2 protein according to PLoS O…

    Promoter

    UbC

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p2Bac.HA.p52

    Plasmid

    #39219

    Depositor

    Aziz Sancar

    Insert

    p52 (GTF2H4 Human)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Pp10

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pKG187

    Plasmid

    #63077

    Purpose

    Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    FAAP24, IMAGE clone 3659284 (FAAP24 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SMYD3

    Plasmid

    #32048

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SMYD3 (SMYD3 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

36. pSpCas9_mouse_Adar1_ccB

    Plasmid

    #158122

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA5/FRT/TO SBBP-CDK1 WT (2007)

    Plasmid

    #61847

    Purpose

    mammalian expression of CDK1 WT, used for Ips

    Depositor

    Jonathon Pines

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pLV Cnep1r1-V5

    Plasmid

    #175133

    Purpose

    Lentiviral expression of mouse Cnep1r1-V5

    Depositor

    Larry Gerace

    Insert

    Cnep1r1 (Cnep1r1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 4MTL

    Plasmid

    #60100

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    METTL21C:PBC002-E06:C229066 (METTL21C Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

40. 4P7A

    Plasmid

    #61954

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    

    Use

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    March 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

41. pENTRY4_HuB

    Plasmid

    #65752

    Purpose

    entry vector with HuB

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGEX-6P-Cdh1wt (570)

    Plasmid

    #39877

    Depositor

    Jonathon Pines

    Insert

    FZR1 (FZR1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

43. isoform PKC Zeta 2 (JCE863)

    Plasmid

    #182028

    Purpose

    yeast expression of mouse PKC Zeta isoform 2 with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Insert

    PKC Zeta isoform 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLEX307-CTSL-G418

    Plasmid

    #158468

    Purpose

    Constitutive lentiviral expression of human CTSL.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    CTSL (CTSL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHyg-PCUP1-AID*(N)

    Plasmid

    #99529

    Purpose

    N-terminal AID* degron cassette with CUP1 promoter and HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Insert

    IAA17(71-117) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    Expression

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. ENO3

    Plasmid

    #39072

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ENO3 (ENO3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

47. pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

    Plasmid

    #44081

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    12xHis tag, 6xGly linker, ADH1 transcription term…

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pTre-Tight-luciferase-Hottip (522)

    Plasmid

    #65490

    Purpose

    positive control for lncRNA expression assay

    Depositor

    Rory Johnson, Roderic Guigo

    Insert

    Hottip (HOTTIP Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSpot-Tag-Lamin

    Plasmid

    #117062

    Purpose

    For mammalian expression of lamin B1 with N-terminal Spot-Tag

    Depositor

    Felix Hartlepp

    Insert

    Lamin B1 (LMNB1 Human)
    

    Use

    Tags

    Spot-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pENTRY4_HuD

    Plasmid

    #65754

    Purpose

    entry vector with HuD

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge