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Showing: 2601 - 2620 of 65344 results

  1. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    John Doench, David Root
    Publication
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    96921-plasmid-map-sequence-id-181715

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  2. MO91-dCas9-AID(P182X)

    Plasmid
    #83260
    Purpose
    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.
    Depositor
    Xing Chang
    Publication
    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.
    Insert
    dCas9-AID P182X
    Use
    Retroviral
    Tags
    3xFLAG-NLS and FLAG
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9; P182X in AID
    Promoter
    MSCV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  3. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid
    #80768
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.
    Depositor
    Kazuhisa Nakayama
    Publication
    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20 Articles
    80768-plasmid-map-sequence-id-171038

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  4. PB-TAC-ERN

    Plasmid
    #80475
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111
    Depositor
    Knut Woltjen
    Publication
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    80475-plasmid-map-sequence-id-477521

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  7. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Publication
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    71288-plasmid-map-sequence-id-166343

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  8. MSP2440

    Plasmid
    #72250
    Purpose
    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    72250-plasmid-map-sequence-id-131871

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  17. pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

    Plasmid
    #85583
    Purpose
    cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)
    Depositor
    Paul Sternberg
    Publication
    Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    Prab-3-GAL4-SK(DBD)-VP64
    Expression
    Worm
    Promoter
    Prab-3-GAL4-SK(DBD)-VP64
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    85583-plasmid-map-sequence-id-170931

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  20. pGD-p19

    Plasmid
    #196326
    Purpose
    35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor
    Depositor
    Zhenghe Li
    Publication
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    tomato bushy stunt virus p19
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196326-plasmid-map-sequence-id-390023

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Showing: 2601 - 2620 of 65344 results