We narrowed to 15,732 results for: LEA

1. Reb_79 aka PAS620

   Plasmid

   #71225

   Purpose

   Reb_1 with RebA(Q91R)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebA( G53A, Q91R)

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pKmyc-Exp5

   Plasmid

   #12552

   Depositor

   Ian Macara

   Article

   Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

   Insert

   Exportin-5 (XPO5 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Oct. 2, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGFP-HBx

   Plasmid

   #24931

   Depositor

   Xin Wang

   Article

   Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

   Insert

   HBx
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRESneo-FLAG/HA EYFP

   Plasmid

   #10825

   Depositor

   Thomas Tuschl

   Article

   Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

   Insert

   EYFP
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ec dnaN-pHK/P

   Plasmid

   #237239

   Purpose

   over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)

   Depositor

   Michael O'Donnell

   Insert

   dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
   

   Tags

   his-10+PKA kinase motif+PreScission Protease clea…

   Expression

   Bacterial

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOpen-BsupolLF

   Plasmid

   #165563

   Purpose

   Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

   Depositor

   FreeGenes Project, Jennifer Molloy, Drew Endy

   Insert

   Bsu DNA Polymerase I, Large Fragment
   

   Use

   Synthetic Biology

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. p3-K230-FokI_L (DAS)

   Plasmid

   #32546

   Depositor

   Jin-Soo Kim

   Article

   Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

   Insert

   ZFN
   

   Tags

   HA epitope

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Dec. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pNIC- AVITAG-CTH10STII- -GG

   Plasmid

   #215805

   Purpose

   Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3-miR29b

   Plasmid

   #21121

   Depositor

   Joshua Mendell

   Article

   Hwang et al Science. 2007 Jan 5. 315(5808):97-100.

   Insert

   miR-29b-1 (MIR29B1 Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pET11b_MutT

    Plasmid

    #73155

    Purpose

    Expresses in E. coli, E coli MutT

    Depositor

    Sandra Gabelli, Maurice Bessman

    Article

    Bessman et al J Biol Chem. 1996 Oct 11;271(41):25059-6

    Insert

    MutT
    

    Expression

    Bacterial

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCAG_eGCaMP(plus)

    Plasmid

    #201147

    Purpose

    expresses eGCaMP+ in mammalian cells

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP+
    

    Expression

    Mammalian

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAP2

    Plasmid

    #171208

    Purpose

    bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease

    Depositor

    Geraldine Seydoux

    Article

    Schmidt et al bioRxiv 2020.10.15.340570

    Insert

    pgl-3 (pgl-3 Nematode)
    

    Tags

    MBP::HIS::TEV cleavage site

    Expression

    Bacterial

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Hbxadr-Hatag

    Plasmid

    #24930

    Depositor

    Xin Wang

    Article

    Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

    Insert

    HBx
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

14. p3-K230-FokI_R (wt)

    Plasmid

    #32545

    Depositor

    Jin-Soo Kim

    Article

    Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

    Insert

    ZFN
    

    Tags

    HA epitope

    Expression

    Mammalian

    Available Since

    Dec. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

15. Reb_221 aka PAS628

    Plasmid

    #71233

    Purpose

    Reb_1 with RebA(G90A)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA(G53A, G90A)

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pG1AK

    Plasmid

    #71736

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Expression

    Bacterial

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTV-Oct4-TK-HMS

    Plasmid

    #62351

    Purpose

    Mouse Oct4 targeting vector

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    TK-Hyg-24xMS2
    

    Use

    Mouse Targeting

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Reb_80 aka PAS621

    Plasmid

    #71226

    Purpose

    Reb_1 with RebB(I67T, N84I)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebB(I67T, N84I)

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

19. Reb_140 aka PAS625

    Plasmid

    #71230

    Purpose

    Reb_1 with RebA(S88P)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA(S88P)

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pNIC-NHSTIIT-AVITAG-GG

    Plasmid

    #215802

    Purpose

    Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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