We narrowed to 28,899 results for: PLE
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Plasmid#178981PurposepACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR arrayDepositorInsertCRISPR array from Candidatus Scalindua brodae
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL4 CTF-FLAG
Plasmid#217709PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL4 (ADGRL4 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-406PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB2 CTF-FLAG
Plasmid#217682PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB2 (BAI2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-911PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro
Plasmid#192683PurposeLentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS1034
Plasmid#63796PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection markerDepositorInsertCBP-3xFLAG-TEV-Protein A followed by KlLEU2
UseE. coli cloning vectorTagsCBP-3xFLAG-TEV-Protein AExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10714
Plasmid#183959PurposeU6-crKlf4-CAG-hyperdCas12a-miniVPRDepositorInsertsHyperdCas12a
crRNA targeting Klf4
UseCRISPRTagsHAExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAG and U6Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXSIP-GPR35-FLAG-APEX
Plasmid#204706Purposeexpresses GPR35-FLAG-APEX in mammalian cellsDepositorInsertG protein-coupled receptor 35 (GPR35 Human)
UseTagsFLAG, APEXExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry
Plasmid#84260PurposeExpresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulationDepositorInsertsSp sgSV40
PYL1-KRAB
UseCRISPR and LentiviralTagsIRES-mCherry and PYL1ExpressionMammalianMutationPromoterCMV and mouse U6Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G2-RacE
Plasmid#188966PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
racE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Arp2_pLib
Plasmid#173676PurposeArp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArp2 (ACTR2 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_1
Plasmid#103919PurposeProtein expression and purification of PTN13 PDZ1 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_3
Plasmid#103921PurposeProtein expression and purification of PTN13 PDZ3 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_2
Plasmid#103920PurposeProtein expression and purification of PTN13 PDZ2 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPN13_PDZ_5
Plasmid#103923PurposeProtein expression and purification of PTN13 PDZ5 domainDepositorInsertPTPN13 (PTPN13 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
LN-entry
Plasmid#128012PurposeVector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag
UseLamdan entry vectorTagsLN-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJN601
Plasmid#59790Purposezf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a markerDepositorInsertsZF1 domain from pie-1
N-terminal half of yfp with introns
intron containing floxed unc-119 gene in reverse orientation
C-terminal half of yfp with introns
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXW117As(RS-E11)
Plasmid#123145PurposeArsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ117, ParsR, PhrpLE, Pecf11Available sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-MCS-GAL4-MiniWhite
Plasmid#165890PurposePuromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.DepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-G protein Gamma 5
Plasmid#42200DepositorInsertGamma 5 (Gng5 Rat)
UseTagsVenus(1-155)ExpressionMammalianMutationcodon-optimizedPromoterCMVAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSR06
Plasmid#69152Purposeread-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr IIDepositorInsertsmCherry
eGFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0 and codon-optimzed index…Promoterrps-27Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
8200 Bicistronic_GFP_ires_hygro
Plasmid#64375PurposeThis a retroviral expression plasmid expressing GFP along with hygro resistance geneDepositorInsertEGFP
UseRetroviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC39
Plasmid#62333PurposesgRNA + 1x PP7 with mCherry for mammalian cellsDepositorInsertssgRNA + 1x PP7
mCherry
UseLentiviralTagsExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Arp3_pLib
Plasmid#173677PurposeArp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArp3 (ACTR3 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag
Plasmid#82469PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive formDepositorInsertmRNA capping enzyme (Rngtt Mouse)
UseTagsFlag and MycExpressionMammalianMutationchanged Lysine 294 to AlaninePromoterCMVAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-myc NES-mCE NLS-Flag
Plasmid#82468PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzymeDepositorInsertMyc-NES-mCE-Flag (Rngtt HIV, Mouse)
UseTagsFlag and MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPK-351
Plasmid#157921PurposepcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBDDepositorInsertsPIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD
UseSynthetic Biology; OptogeneticsTagsHA tag and SV40NLSExpressionMammalianMutationpif3 1-523PromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc NES- mCE(K294A)
Plasmid#82464PurposeExpresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)DepositorInsertNES mRNA capping enzyme (Rngtt HIV, Mouse)
UseTagsmycExpressionMammalianMutationK294APromoterCMVAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-myc- mCE(Del 25C)
Plasmid#82472PurposeExpresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminalDepositorInsertmyc-mCE-C terminal deleted (Rngtt Mouse)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only