We narrowed to 5,634 results for: pcas

1. ABE-RA3.0

   Plasmid

   #208301

   Purpose

   Expresses ABE-RA3.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA3.0-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX459-CDKN1A

   Plasmid

   #217457

   Purpose

   For CDKN1A (p21) knockout

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   CRISPR Cas9 guide for CDKN1A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-NeuroD6iresGFP

   Plasmid

   #45027

   Depositor

   Connie Cepko

   Article

   Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

   Insert

   NeuroD6 (Neurod6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRANS_232

   Plasmid

   #91121

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

   Plasmid

   #183027

   Purpose

   B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABE-RA2.0

   Plasmid

   #208299

   Purpose

   Expresses ABE-RA2.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA2.0-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ABE-RA3.1

   Plasmid

   #208302

   Purpose

   Expresses ABE-RA3.1 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA3.1-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p15A_SpyCas9_CmR

   Plasmid

   #119162

   Purpose

   Expresses S. pyogenes Cas9

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   Cas9 from S. pyogenes (NEWENTRY )
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT128c

   Plasmid

   #138512

   Purpose

   P2

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   inteinC-dSpCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRiA4-VIR

    Plasmid

    #138193

    Purpose

    Virulence helper compatible with pCambia binary vectors.

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    vir gene cluster
    

    Use

    Vir gene expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ePE6d

    Plasmid

    #229029

    Purpose

    Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice

    Depositor

    Li Juan

    Article

    Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBR322-Pdp1_NTD-Cas9

    Plasmid

    #198275

    Purpose

    For loading SpCas9 into PVCs; also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

    Plasmid

    #71878

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC-delta (Otc Rat)
    

    Use

    Tags

    Expression

    Mutation

    Deleted amino acid 30-114 from full length OTC

    Promoter

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR-dCas9

    Plasmid

    #125687

    Purpose

    Gene knockdown for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spdCas9, sgRNA casette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLSLGFP.R

    Plasmid

    #51501

    Purpose

    Single component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Insert

    GFP
    

    Use

    Plant t-dna plasmid

    Tags

    Expression

    Mutation

    Promoter

    2x35S

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-MALT1-/pRK5

    Plasmid

    #27971

    Depositor

    Xiaolu Yang

    Article

    Kawadler et al Mol Cell. 2008 Aug 8. 31(3):415-21.

    Insert

    MALT1 (MALT1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pKIR1.0

    Plasmid

    #86409

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFPL-Ch

    Plasmid

    #61647

    Purpose

    Gateway vector for generating cyan fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced cyan fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCX-H2B-KikGR

    Plasmid

    #98649

    Purpose

    Expresses a nuclear-localized photoconvertible green-to-red fluorescent protein

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

    Insert

    KikGR
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE enhancer chick beta actin promoter

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

20. PRDX6A-c001

    Plasmid

    #175474

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX6 (PRDX6 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    See Depositor Comments

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTRANS_220d

    Plasmid

    #91114

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTRANS_212

    Plasmid

    #91111

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKD013

    Plasmid

    #207931

    Purpose

    Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braiding

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. px459V2.0_Conc2

    Plasmid

    #134451

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSQT817

    Plasmid

    #53373

    Purpose

    wildtype Cas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. UAS-mNAMPT

    Plasmid

    #187887

    Purpose

    Gal4/UAS expression of mammalian NAMPT

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    mNAMPT
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTRANS_210d

    Plasmid

    #91109

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pScI_dCas9-CDA

    Plasmid

    #108549

    Purpose

    Bacterial Target-AID vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGJJ001

    Plasmid

    #183619

    Purpose

    bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge