We narrowed to 23,431 results for: Spr;

1. pUC18-mini-Tn7T-Plac-dCas9 (Plac)

   Plasmid

   #105235

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Promoter

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCI108-SiT-Cas12a-[Cond]

   Plasmid

   #128407

   Purpose

   Conditionally express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCI118-SiT-Cas12a-[Ind]

   Plasmid

   #128406

   Purpose

   Inducible expression of both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BPK2139

   Plasmid

   #65776

   Purpose

   Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCFD1-dU6:1gRNA

   Plasmid

   #49408

   Purpose

   expression of gRNA under control of the Drosophila U6:1 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-1:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

   Plasmid

   #117134

   Purpose

   All-in-one expression vector for Cas9 and gRNA against CD44

   Depositor

   Kosuke Yusa

   Insert

   gCD44 v2 (Cd44 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sgRNA 2 GAPDH

   Plasmid

   #83809

   Purpose

   Express Sg-2 sgRNA targeting GAPDH

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBLO1811_arC9_noNLS_human

   Plasmid

   #74494

   Purpose

   Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   arC9
   

   Use

   CRISPR

   Tags

   t2a mCherry (C-terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LbdCas12a KRAB

   Plasmid

   #188503

   Purpose

   Expresses FLAG tagged LbdCas12a KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DR274-eGFP sgRNA

    Plasmid

    #61051

    Purpose

    for generation of a eGFP specific sgRNA from a T7 promoter

    Depositor

    Filippo Del Bene

    Article

    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

    Insert

    EGFP sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEmCherry2

    Plasmid

    #118413

    Purpose

    Red fluorescent reporter for assessing CRISPR gRNA activity

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAN-PTet-dCas9

    Plasmid

    #62244

    Purpose

    controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    catalytically inactive, D10A, H840A

    Promoter

    Ptet

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

    Plasmid

    #64112

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74010

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5xUAS
    Cas9
    Cre
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. c3GIC9

    Plasmid

    #62191

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid

    #74705

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKM126

    Plasmid

    #105134

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NAX_CAGGS_CAS9_Puro

    Plasmid

    #167863

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRU206

    Plasmid

    #167693

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::asCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRU322

    Plasmid

    #167683

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge