We narrowed to 4,271 results for: pme

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hRad51(A89E)–Cas9(D10A)

Plasmid

#125563

Purpose

Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(A89E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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AMPK(T>A)-SPARK

Plasmid

#226256

Purpose

phospho-null mutant of AMPK-SPARK

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

AMPK(T>A)-SPARK

Expression

Mammalian

Mutation

substituted Threonine 20 with Alanine

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRSetB-KanR-LiLac

Plasmid

#184569

Purpose

Fluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expression

Depositor

Gary Yellen

Article

Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

Insert

LiLac fluorescent sensor of lactate

Tags

His7

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Sst44-GFP

Plasmid

#172310

Purpose

SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

Depositor

Michael Greenberg

Article

Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

Insert

Sst44

Use

AAV

Promoter

pB-Globin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TetOn-dCas9-KRAB

Plasmid

#115545

Purpose

Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Type

Empty backbone

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7

Plasmid

#170141

Purpose

Expresses CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

6xHis and FLAG

Expression

Insect

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

Plasmid

#71720

Purpose

Expressing an mRuby-tagged TIR1 in the soma of C. elegans

Depositor

Abby Dernburg

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)

Tags

mRuby

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

eef-1A.1 (eft-3)

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Puro-iNgn3

Plasmid

#75337

Purpose

doxycycline inducible Ngn3 over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

neurogenin 3 (Neurog3 Mouse)

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-tdTomato-FlagAkt1

Plasmid

#64831

Purpose

Lentiviral expression of murine Flag-Akt1

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt1 (Akt1 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pPyCAG-EGFP-TM-IRES-Pac

Plasmid

#183603

Purpose

Mammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Tags

Human PDGFRB transmembrane domain and Mouse IgGK …

Expression

Mammalian

Promoter

CAG

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCB24 pDEST crybb1:TagBFP

Plasmid

#195987

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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791 p3E-2A-pCS2MCSpA v2

Plasmid

#195973

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-multiple cloning site

Use

Other

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I35 p35S-XVE-pEstradiol GG0-GG1

Plasmid

#195904

Purpose

Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S-XVE-LexA-p35S

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

Plasmid

#206213

Purpose

PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

Depositor

Masamichi Kamihira

Article

Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

Insert

tetracycline-responsive transactivator

Expression

Mammalian

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCK051 p5E exorh:mCerulean

Plasmid

#195952

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:mCerulean

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Expression

Mammalian

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-mBAF45c IRES GFP

Plasmid

#17882

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45c (Dpf3 Mouse)

Tags

FLAG and IRES EGFP

Expression

Mammalian

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-mBAF45b IRES GFP

Plasmid

#17881

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45b (Dpf1 Mouse)

Tags

FLAG and IRES EGFP

Expression

Mammalian

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only
Bmi1-overexpression

Plasmid

#21577

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Tags

ires-eGFP

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pIVT/Smarcc1

Plasmid

#122145

Purpose

For making Smarcc1 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarcc1 (Smarcc1 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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