4,177 results

1. TetO-FUW-cMYC

   Plasmid

   #20324

   Depositor

   Rudolf Jaenisch

   Article

   Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

   Insert

   cMYC (Myc Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

   Plasmid

   #139996

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-SFFV-dCas9-BFP

   Plasmid

   #46910

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, and HA

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET His6 Sumo TEV LIC cloning vector (2S-T)

   Plasmid

   #29711

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis, SUMO, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEN-CMV-CjCas9

   Plasmid

   #89752

   Purpose

   Expression of CjCas9 in mammalian cells

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40-HA

   Expression

   Mammalian

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-p53-shRNA-427

   Plasmid

   #25636

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   p53-shRNA-427 (TP53 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV_NLS-dSaCas9-NLS-VPR

   Plasmid

   #68495

   Purpose

   AAV vector containing nuclease null SaCas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   dSaCas9
   

   Use

   AAV

   Tags

   VPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEZT-BM

   Plasmid

   #74099

   Purpose

   Multi-purpose rapid titer bacmam expression vector

   Depositor

   Ryan Hibbs

   Article

   Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Bacmam, baculovirus

   Tags

   EGFP following p10 promoter

   Expression

   Mammalian

   Promoter

   CMV, p10

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 LIC cloning vector (6A)

   Plasmid

   #30124

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-Talin1

    Plasmid

    #26724

    Depositor

    Anna Huttenlocher

    Article

    Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

    Insert

    Talin (Tln1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIN BRCA2 WT

    Plasmid

    #16245

    Depositor

    Mien-Chie Hung

    Article

    Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

    Insert

    BRCA2 (BRCA2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1236 pcDNA3 Myr HA Akt3

    Plasmid

    #9017

    Depositor

    William Sellers

    Insert

    Akt3 (AKT3 Human)
    

    Tags

    HA and Myr

    Expression

    Mammalian

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-nCas9n-nanos 3’UTR

    Plasmid

    #62542

    Purpose

    expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

    Insert

    nanos 3' UTR (nanos3 Zebrafish)
    

    Use

    CRISPR

    Mutation

    3' UTR only

    Promoter

    SP6

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV5B-Flag-Smad3

    Plasmid

    #11742

    Depositor

    Jeff Wrana

    Article

    Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

    Insert

    SMAD3 (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 7, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBABE puro K-Ras V12

    Plasmid

    #9052

    Purpose

    Retroviral expression of a constitutively active form of K-Ras

    Depositor

    William Hahn

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V. Constitutively active.

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFR_CrPV_xb

    Plasmid

    #11509

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Available Since

    March 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-SpCas9-NG

    Plasmid

    #117919

    Purpose

    Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ143

    Plasmid

    #69295

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

    Plasmid

    #141385

    Purpose

    Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-E (E SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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