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pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

Plasmid

#141378

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

13 Articles

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pC019 - LwCas13a from Leptotrichia wadei

Plasmid

#91909

Purpose

Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a and guide expression cassette

Use

CRISPR

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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EGFP-CAAX

Plasmid

#86056

Purpose

A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Homo sapiens Harvey rat sarcoma (HRAS Human)

Tags

GFP

Expression

Mammalian

Mutation

contains the region from 825-857 of HRAS as a far…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV-nEFCas9

Plasmid

#87115

Purpose

SpCas9 expression AAV backbone plasmid under control of nEF promoter

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

nEF-Cas9

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pDECKO_mCherry

Plasmid

#78534

Purpose

Backbone to clone single or paired sgRNAs

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA)

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Tags

GFP11x7 and mCherry

Expression

Insect

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Publication

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-6xHis-(-30)GFP

Plasmid

#62936

Purpose

Expression of 6xHis-(-30)GFP in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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ptf-Galectin3

Plasmid

#64149

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Publication

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Galectin3 (LGALS3 Human)

Tags

EGFP and mRFP

Expression

Mammalian

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCAG-VSVP

Plasmid

#64088

Purpose

Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

Depositor

Ian Wickersham

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VSV phosphoprotein

Expression

Mammalian

Promoter

CAG

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Publication

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Mutation

*see comment below

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-Ef1a-fDIO EYFP

Plasmid

#55641

Purpose

Flp-Dependent EYFP

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has service

AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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FLAG-Stx17

Plasmid

#45911

Depositor

Noboru Mizushima

Publication

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Syntaxin 17 (STX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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PU6::unc-119_sgRNA

Plasmid

#46169

Purpose

unc-119 targeting sgRNA

Depositor

John Calarco

Publication

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

unc-119 targeting sgRNA (unc-119 Synthetic)

Use

CRISPR

Promoter

C. elegans U6 snRNA pol III promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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LeGO-Cer2

Plasmid

#27338

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Cerulean

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pLKO-puro FLAG SREBP2

Plasmid

#32018

Depositor

David Sabatini

Publication

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCS2 HRS-RFP

Plasmid

#29685

Depositor

Edward De Robertis

Publication

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)

Tags

RFP

Expression

Mammalian

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Arp3-pmCherryC1

Plasmid

#27682

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Arp3 (ACTR3 Human)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pDONR223-PDGFRA

Plasmid

#23892

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRA (PDGFRA Human)

Use

Gateway Cloning

Mutation

M260I

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Publication

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Expression

Bacterial and Mammalian

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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