165,307 results

1. pOpen-MMLV_RT (lack H)

   Plasmid

   #165556

   Purpose

   Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS

   Plasmid

   #158741

   Purpose

   Expresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

   Insert

   Hemagglutinin A/MI/45/15 H1N1
   

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   AA61-469

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PJ-DEAD

   Plasmid

   #38002

   Depositor

   Robin Irvine

   Article

   Hammond et al Science. 2012 Jun 21.

   Inserts

   FKBP1A (FKBP1A Human)
   SAC1 (SAC1 Budding Yeast)
   INPP5E (INPP5E Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Asp 1263 mutated to Ala and Cys 779 mutated to Ser

   Available Since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-IGFBP5-V5

   Plasmid

   #11608

   Depositor

   Steven Johnson

   Insert

   Insulin Growth Factor Binding Protein 5 (IGFBP5 Human)
   

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

5. RHCglo

   Plasmid

   #80169

   Purpose

   Minigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing

   Depositor

   Thomas Cooper

   Article

   Singh et al Biotechniques. 2006 Aug;41(2):177-81.

   Insert

   segments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXJ-His-CD276

   Plasmid

   #232662

   Purpose

   transient expresses CD276 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   CD276 (CD276 Human)
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

   Plasmid

   #218528

   Purpose

   sgRNA targeting human EIF2AK4/GCN2

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK4 (EIF2AK4 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Influenza-FPV-HA-mEGFP

   Plasmid

   #127810

   Purpose

   Expression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localization

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion
   

   Expression

   Mammalian

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZalphaB-SapL3

   Plasmid

   #78171

   Purpose

   Expression of Saporin L3 in pichia

   Depositor

   Vern Schramm

   Article

   Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

   Insert

   Saporin L3
   

   Tags

   alpha factor

   Expression

   Yeast

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. phMYT1L-N174

    Plasmid

    #66809

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    MYT1L (MYT1L Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human EF1a

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTHSSe_50

    Plasmid

    #109248

    Purpose

    incW BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV sgRNA Expression Plasmid

    Plasmid

    #174540

    Purpose

    Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

    Depositor

    Ormond MacDougald

    Article

    Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmiRGLO-Zc3h12a 3’UTR

    Plasmid

    #222662

    Purpose

    Luciferase reporter vector containing mouse Zc3h12a 3'UTR

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a 3'UTR (Zc3h12a Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-CMV-Galpha-oA ONE-GO

    Plasmid

    #189732

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-oA ONE-GO
    GNAO (Gnao1 Rat)
    

    Use

    Lentiviral

    Mutation

    I250 (silent mutation in YFP)

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3-TrxRFP2

    Plasmid

    #172322

    Purpose

    an improved fluorescent biosensor for thioredoxin redox

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    TrxRFP2, an improved version from TrxRFP1
    

    Tags

    His-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FU-tet-o-hc-myc

    Plasmid

    #19775

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. AiP13786 - pAAV-AiE0140h-minBG-iCre(R297T)-BGHpA (Alias: CN3786)

    Plasmid

    #230382

    Purpose

    AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minRho

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc-sgRNA-Cas9

    Plasmid

    #49330

    Purpose

    Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. mNeonGreen-Giantin

    Plasmid

    #98880

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Giantin
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc

    Plasmid

    #21850

    Depositor

    David Ron

    Article

    Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    ATF4 5' UTR and AUG fused to Luciferase driv…

    Available Since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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