161,570 results

1. pmTurquoise2-Tubulin

   Plasmid

   #36202

   Purpose

   In vivo visualization of microtubules (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   Alpha-tubulin (TUBA1B Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-MEG3

   Plasmid

   #44727

   Depositor

   Anne Klibanski

   Article

   Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

   Insert

   Maternally Expressed Gene 3 (MEG3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-EGFP

   Plasmid

   #50465

   Purpose

   EGFP under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial size

   Insert

   EGFP
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. BiP-mGFP

   Plasmid

   #62231

   Purpose

   Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.

   Depositor

   Erik Snapp

   Article

   Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

   Insert

   hamster BiP-mGFP-KDEL
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDual_dsCas9_Blast

   Plasmid

   #214681

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. v3em-Cterm-PE2max-∆RNaseH-dualU6

   Plasmid

   #198735

   Purpose

   AAV genome encoding C-terminal PE2max and U6 expression cassettes

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   NpuC-CtermPE2max∆RNaseH
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cbh

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tag5Amyc-GSK3b WT

   Plasmid

   #16260

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   GSK3 beta (GSK3B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-FLAG-p14

   Plasmid

   #42330

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   p14 (LAMTOR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYTK001

   Plasmid

   #65108

   Purpose

   Part Plasmid Entry Vector to be used in the Dueber YTK system.

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   none
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    U6-96Ab and hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Plasmid

    #180475

    Purpose

    Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Zebrafish ubb promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131004

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS U3-hTR-500

    Plasmid

    #28170

    Depositor

    Kathleen Collins

    Article

    Fu et al Mol Cell. 2003 May . 11(5):1361-72.

    Insert

    U3-hTR-500 (TERC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-DAAO

    Plasmid

    #60344

    Purpose

    Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

    Depositor

    Vsevolod Belousov

    Article

    Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

    Insert

    DAAO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3-EF1A-mTert

    Plasmid

    #162555

    Purpose

    Overexpression of Tert

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of Tert (Tert Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKL123

    Plasmid

    #187889

    Purpose

    AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9

    Depositor

    Martin Kampmann

    Article

    Leng et al Nat Neurosci. 2022 Nov;25(11):1528-1542.

    Insert

    NFIA-SOX9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Hs.cargo(Cre)

    Plasmid

    #174863

    Purpose

    Expresses human Peg10 cargoRNA encoding Cre recombinase

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    Cre flanked by HsPeg10 UTRs
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p4xmts-mScarlet3_N1

    Plasmid

    #189774

    Purpose

    Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    4xmts-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-HA-Flag-natT1R2

    Plasmid

    #113944

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIS0

    Plasmid

    #12178

    Purpose

    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

    Depositor

    David Bartel

    Article

    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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