-
-
qTAG-C-mStayGold-Blast
Plasmid#227254PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7RNAP
Plasmid#74096PurposeT7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNADepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
EZ-Tet-pLKO-Puro
Plasmid#85966PurposeTet/Dox inducible shRNA lentivirus with puromycin selectionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterH1 / TetOAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-tag (m) Vector
Plasmid#101135PurposeExpression of protein of interest with SNAP-Tag in Mammalian cellsDepositorTypeEmpty backboneUseTagsSNAP-tag (SNAP26m)ExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdTomato-ZO1-C-14
Plasmid#58147PurposeLocalization: Tight Junctions, Excitation: 554, Emission: 581DepositorInsertZO1 (TJP1 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-Membrane-1
Plasmid#57992PurposeLocalization: Plasma Membrane, Excitation: 555, Emission: 584DepositorTypeEmpty backboneUseTagsN-terminal 20 Aas MLS from Neuromodulin and mTagR…ExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBT346_pLambda-attB-pCA-GFP-pA
Plasmid#52551Purposefor producing PhiC31 attB-containing minicircle in vitroDepositorInsertsLambda integrase attL1
Lambda integrase attR1
PhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
tetO.Sox9.Puro
Plasmid#117269PurposeExpresses Sox9 and puromycin resistance gene under control of tetO sequence.DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsT2A and puromycin resistant cassette (C terminal …ExpressionMammalianMutationPromotertet-onAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
XLone-SOX17
Plasmid#135764Purposeexpresses human SOX17 upon doxycycline treatmentDepositorInsertSOX17 (SOX17 Human)
UseTagsExpressionMammalianMutationnonePromoterTRE3GAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
VEGFR2-mCh
Plasmid#108854PurposeEncodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linkerDepositorInsertKDR (KDR Human)
UseTagsLabeled with mCherry on the C-Terminus via a 3 am…ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCpfSA
Plasmid#187890PurposeCRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChR2-YFP
Plasmid#171622PurposeTRE-ChR2-YFP reporterDepositorInsertTRE-ChR2-YFP
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_WT USP7
Plasmid#131242PurposeMammalian expression of N-terminally Myc-tagged USP7DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationPromoterCMV enhancer + CMV promoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AncBE4stem
Plasmid#208766PurposeAncBE4max that co-expresses UGI and p53DDDepositorInsertAncBE4max with 2A-UGI and 2A-p53DD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-MAPPER
Plasmid#117721PurposeMAPPER Selectively Labels ER-PM JunctionsDepositorInsertMAPPER
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL528
Plasmid#58495Purposehelper plasmid for bacterial conjugal DNA transferDepositorInsertencodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
UseTagsExpressionMutationPromoterAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2lox-cAMPr
Plasmid#99143PurposeExpresses cAMPr in mammalian cellsDepositorInsertcAMPr
UseCre/LoxTagscpGFPExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only
