166,041 results

1. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6PBG

   Plasmid

   #162263

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   COPA (COPA Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET21a-Streptavidin-Alive

   Plasmid

   #20860

   Purpose

   Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Alive
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Wt gene

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. MT363

   Plasmid

   #65858

   Purpose

   The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

   Depositor

   Detlef Weigel

   Article

   Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

   Insert

   nahG
   

   Expression

   Plant

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-ConVERGD-(HA)hM3Dq-W3SL

   Plasmid

   #218758

   Purpose

   Provides AND intersectional (Cre+Flp) expression of HA-hM3Dq

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   hM3Dq
   

   Use

   AAV

   Tags

   HA

   Promoter

   hSyn

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-PinkyCaMP

   Plasmid

   #232860

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CAG promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. S96 ELP

   Plasmid

   #68393

   Purpose

   Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   S96 ELP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPGKPhiC31obpA

   Plasmid

   #13795

   Purpose

   Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

   Depositor

   Philippe Soriano

   Article

   Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

   Insert

   PhiC31o
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB4581

   Plasmid

   #171124

   Purpose

   A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

   Insert

   3_sAID
   

   Use

   Other

   Mutation

   non applicable

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9-EGFP

    Plasmid

    #232761

    Purpose

    Expresses dCas9-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-EGFP
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pESC-URA (V5 and HA)

    Plasmid

    #222485

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and URA3 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Tags

    HA and V5

    Expression

    Yeast

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-EGFP-C4-Nrf2

    Plasmid

    #21549

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-GRAB_g5-HT3.0

    Plasmid

    #208717

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Has Service

    AAV1, AAV2, and AAV8

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-EGFP

    Plasmid

    #27082

    Purpose

    Integration-free (episomal) expression of EGFP

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    eGFP
    

    Expression

    Mammalian

    Available Since

    March 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. JAK2 (V617F)-pcw107-V5

    Plasmid

    #64610

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    V617F

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST51-LRRK2-WT

    Plasmid

    #25080

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Destination vector

    Tags

    6XHis and V5

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. V96 ELP

    Plasmid

    #68392

    Purpose

    Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    V96 ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEVA63_HelperΔgIII

    Plasmid

    #190433

    Purpose

    M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Insert

    M13 genes I-V and VII-XI
    

    Use

    Synthetic Biology

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PLKO.1-UPF1-3'UTR

    Plasmid

    #136036

    Purpose

    UPF1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (TTATTACCCAGAATAAGATGC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-Neo-TRE-CMV-Cre-rtTA

    Plasmid

    #165457

    Purpose

    Tetracycline inducible CRE expression from AAVS1 locus

    Depositor

    Madeline Lancaster

    Article

    Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

    Insert

    CRE
    

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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