Addgene

We narrowed to 8,061 results for: gnal

Showing: 4021 - 4050 of 8061 results

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pGlomyc-Frat2

Plasmid

#124500

Purpose

Expresses myc-tagged mouse FRAT2 in mammalian cells (N-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Frat2 (Frat2 Mouse)

Use

Tags

myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5-rat ERK2-S151D

Plasmid

#40818

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

S151D

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
px330 CTCF gRNA

Plasmid

#165588

Purpose

Insertion of CTCF degon

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

CTCF (CTCF Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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ETV3_pet28a

Plasmid

#131646

Purpose

Bacterial expression of ETS gene ETV3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV3 (ETV3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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px330 CBP gRNA

Plasmid

#165587

Purpose

insertin of CBP degrom

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

CBP (Crebbp Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgAdam10 v3

Plasmid

#158034

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV5-rat ERK2-L73P

Plasmid

#40817

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

L73P

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pCEP4-HA-Flag-natT1R2 ECD

Plasmid

#113954

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-568

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBABEhygro-Flag-ZO-2-1st-PDZ-mut

Plasmid

#27474

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

K38E, F44H, I46E and V48E

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/myc-His-A-YAP2-deltaC

Plasmid

#21125

Depositor

Marius Sudol

Article

Tsutomu Oka & Marius et al

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

5 Carboxy-terminal amino acids -FLTWL are deleted

Promoter

Available since

July 2, 2009

Availability

Academic Institutions and Nonprofits only
pDEST53-Arl13b20

Plasmid

#40876

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted Arl N-terminal domain; added stop codon

Promoter

CMV

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only
RET_Y1062F-SHC

Plasmid

#139620

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET_Y1062F (RET Human)

Use

Tags

StrepIII-HA

Expression

Mammalian

Mutation

Y1062F

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-Fos S374A

Plasmid

#118302

Purpose

Expression of mouse C-Fos

Depositor

Philip Stork

Article

Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

Insert

C-Fos S374A (Fos Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

S374A

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRRG319-nlk

Plasmid

#99082

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea nemo-like kinase (also known as h.109.4e)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

nemo-like kinase (also known as h.109.4e) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) NEMO shRNA1

Plasmid

#22505

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

NEMO shRNA1 (Ikbkg Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
p2Flag CMV2-ZO-2-delta-N

Plasmid

#27419

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Deletion of coding nucleotides from 1-351. This r…

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
LP454

Plasmid

#90185

Purpose

pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 1-649

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

NPHP4 (NPHP4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

aa residues 1-649

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-WIP 460 deletion

Plasmid

#11773

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP aa1-460 (WIPF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

aa461-503 deleted

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shLKB1-Ms

Plasmid

#61234

Purpose

Entry clone encoding shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (STK11, Stk11 Mouse, Human)

Use

Entr clone

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

Plasmid

#67687

Purpose

UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

dusp6 (dusp6 Zebrafish)

Use

Tags

6xmyc-NLS-tdTomato-2A

Expression

Mutation

S159A, S196A

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 R419A

Plasmid

#111792

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R419A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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beta4-nAChR shRNA (Beta-4.3)

Plasmid

#86650

Purpose

expression of shRNA targeting CHRNB4

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNB4 (beta4-nAChR) (CHRNB4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT-DEST EVC2dW-TEV-YFP-Flag

Plasmid

#41006

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Use

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

deleted amino acids 1177-1220

Promoter

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCLX-MIFE-hM3Dq-PGK-mCherry

Plasmid

#114294

Purpose

Mifepristone-inducible expression of hM3Dq protein

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

hM3Dq

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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RCAN1-aa1-150

Plasmid

#65412

Purpose

RCAN1 aa1-150 expression with CMV promoter. Flag tagged.

Depositor

Jeffery Molkentin

Article

Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

Insert

RCAN1-aa1-150 (Rcan1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa1-150 present, deleted 151 to end

Promoter

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pSicoR (Puro) NEMO shRNA2

Plasmid

#22506

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

NEMO shRNA2 (Ikbkg Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL VV458/460PP

Plasmid

#61368

Purpose

Expresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with VV458/460P mutation (RIPK3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P and V460to P

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pInducer21 Flag-Sesn2 419A/422A/426A

Plasmid

#61870

Purpose

Dox inducible lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutant

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

419A/422A/426A

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-Cerulean-UCR1

Plasmid

#67578

Purpose

Expresses the UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

Upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Use

Tags

Cerulean and FLAG

Expression

Mammalian

Mutation

Amino acid residues 17-136 of PDE4D3

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-hRIP1-FL I539P

Plasmid

#61380

Purpose

Expresses Human full length RIPK1with I539P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP1with I539P mutation (RIPK1 Human)

Use

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

changed I539 to P

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only