We narrowed to 13,335 results for: ache
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Plasmid#169952PurposeMammalian expression of CARD8-FLAG with a site III mutation (L368G)DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1 LIC 6A CARD8-FLAG E274R
Plasmid#169950PurposeMammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG F370G
Plasmid#169953PurposeMammalian expression of CARD8-FLAG with a site III mutation (F370G)DepositorAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 A59P
Plasmid#166816PurposeGateway pDONR vector encoding NLRP1 with the patient mutation A59P (Herlin et al., 2020) (no stop codon)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159852PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc227
Plasmid#161948PurposeContains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetRDepositorInsertpSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
ExpressionBacterialPromoterPBADAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc367
Plasmid#161947PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20DepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
ExpressionBacterialAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc228
Plasmid#161946PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetRDepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
ExpressionBacterialAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B CARD8 UPA-CARD-mCherry R495E
Plasmid#164016PurposeMammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6B CARD8 UPA-CARD-mCherry Y527A
Plasmid#164018PurposeMammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imagingDepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD A452C
Plasmid#164023PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (A452C mutation for cysteine labelling applications)DepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT-9251
Plasmid#124226PurposeBacterial SpCas9-HF1 expressionDepositorInsertSpCas9-HF1
UseCRISPRExpressionBacterialPromoterTetR/TetAAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM266 - pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Plasmid#159829PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertpEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Plasmid#159833PurposeSingle copy or array insertion by MosTI using split NeoR selectionDepositorInsertpEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfKitlga
Plasmid#140292PurposeExpress soluble His-tagged zebrafish Kitlga in insect cellsDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Plasmid#159838PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arraysDepositorInsertpDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
ExpressionWormMutationNot applicableAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAT2_G-alpha
Plasmid#109947PurposeProtein expression and purification of GNAT2_G-alphaDepositorInsertGNAT2_G-alpha (GNAT2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA3
Plasmid#158481PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codonDepositorAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA2
Plasmid#158480PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codonDepositorAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA11
Plasmid#158489PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codonDepositorAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA9
Plasmid#158487PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codonDepositorAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA4
Plasmid#158482PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codonDepositorAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA10
Plasmid#158488PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codonDepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA12
Plasmid#158490PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codonDepositorAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_3K
Plasmid#131205Purposemammalian expression, tet RDepositorInsertRIPK2 K209/410/538R (RIPK2 Human)
TagsEGFPExpressionMammalianMutationK209/410/538RPromoterpCMVAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Hypa-nCas9n-A69
Plasmid#125645Purposemicro-injection of poly-adenylated Hypa-nCas9n RNADepositorInsertHypa-nCas9n
UseRna in vitro transcription vectorMutationN692A, M694A, Q695A, H698A from wild-type Cas9PromoterT3Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP
Plasmid#124066PurposeE. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.DepositorInsertcytATL-R232Q-sfGFP
TagssfGFPExpressionBacterialMutationR232QPromotertrcAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti4 Blast RFP-PNRC1
Plasmid#123297PurposeMammalian expression of PNRC1 N-RFPDepositorAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51 PNRC1
Plasmid#123293PurposeExpression of PNRC1 in mammalian cellsDepositorInsertPNRC1 (PNRC1 Human)
ExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pmyo-3::hpo-30 gDNA (pBAB204)
Plasmid#117385PurposeAllows for body-wall muscle expression of HPO-30DepositorInsertgenomic DNA of hpo-30 (833 bp)
ExpressionWormAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFL I3
Plasmid#113514PurposeExpression of I3 in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-STOP-HEC1
Plasmid#114026PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.DepositorInserteGFP-STOP-HEC1 (NDC80 Human)
ExpressionMammalianMutationStart codon of Hec1 is mutated to stop codonAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-biotin-myc- mCE
Plasmid#82475PurposeExpresses mRNA capping enzyme containing N terminal biotin and myc tagDepositorAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H
Plasmid#108378PurposeFRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
ExpressionMammalianPromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-TPI_WT-xrRNA-4H
Plasmid#108377PurposeFRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
ExpressionMammalianPromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1474
Plasmid#82420PurposePlasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
TagsHAExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterCMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1475
Plasmid#82421PurposePlasmid for expression of HA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
TagsHAExpressionMammalianMutationdeleted amino acids 1-1158 from nvGW182PromoterCMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1476
Plasmid#82422PurposePlasmid for expression of NHA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
TagsNHAExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterCMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1477
Plasmid#82423PurposePlasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
TagsNHAExpressionMammalianMutationdeleted amino acids 1-1158 from nvGW182PromoterCMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1479
Plasmid#82425PurposePlasmid for expression of GST-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
TagsGSTExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterEF1AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1480
Plasmid#82426PurposePlasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
TagsGSTExpressionMammalianMutationamino acids 1-1158 of nvGW182 were deletedPromoterEF1AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA1_no stop
Plasmid#83847PurposeGateway donor vector for IAA1 with no stop codonDepositorAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio
Plasmid#80325PurposeExpresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tagsDepositorInsertPXDNL-003 (PXDNL Human)
TagsBiotinExpressionMammalianMutationH170A and H256APromoterCMVAvailable SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-AVITEV-GFP
Plasmid#74052Purposeretroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage siteDepositorInsertAVITEV-GFP
UseRetroviralTagsAVI-TEV-tag N-terminal to the GFPExpressionMammalianPromoterpMSCV-LTRsAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_C1 I3(resist)
Plasmid#61399PurposeExpresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
TagsmCherryExpressionMammalianMutationsilent mutations of bases C183T T192C T189CPromoterCMVAvailable SinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3(resist)
Plasmid#61400PurposeExpresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
TagsGFPExpressionMammalianMutationsilent mutations of bases C183T T192C T189CPromoterCMVAvailable SinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-GFP-I3(V41A/W43A)
Plasmid#61402PurposeExpresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cellsDepositorAvailable SinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Keratin-17
Plasmid#55424PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475DepositorAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Lasp1-N-10
Plasmid#55426PurposeLocalization: Focal Adhesions, Excitation: 433, Emission: 475DepositorAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only