We narrowed to 7,692 results for: Lif;
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Plasmid#209400PurposeTransiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
TagsGFPExpressionPlantPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP8H
Plasmid#188062PurposePROSS-designed versatile peroxidase 8HDepositorInsertPROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
TagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
ExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-wt-TTF-1
Plasmid#49989PurposeFor transient transfection to express human wild type TTF-1 proteinDepositorAvailable SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNCS tdTomato
Plasmid#91767PurposeBacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)DepositorInserttandem dimer Tomato
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisB-TRF2
Plasmid#50488PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 (TERF2 Human)
Tags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-500PromoterpTrcAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGG-cTP-TurboID
Plasmid#209394PurposeTransiently expressing TurboID fused with a chloroplast transit peptide (cTP) in plantaDepositorInsertcTP-3XHA-TurboID
ExpressionPlantPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot8
Plasmid#37367DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
Plasmid#74101Purposevector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015DepositorInsertsGreen Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
Available SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMD19-PBBa_J23117-sgRNA-Spr
Plasmid#190793PurposeTemplate vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.DepositorInsertsgRNA (dummy)
UseSynthetic BiologyExpressionBacterialPromoterBBa_J23117Available SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-hXRCC1
Plasmid#52274PurposeBacterial expression of human XRCC1 with N-terminal GST-tagDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr
Plasmid#190794PurposeTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.DepositorInsertsgRNA-scaffold only
UseSynthetic BiologyExpressionBacterialPromoterBBa_J23117Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGex-4T-2_GABARAP
Plasmid#73948PurposeFor recombinant expression of human GABARAP in E.coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSc-humanADAR2
Plasmid#171106PurposeExpresses human ADAR2 in yeast.DepositorAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-3XNLS
Plasmid#209392PurposeTransiently expressing TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
ExpressionPlantPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-SAR1-V5-His6
Plasmid#67451PurposeMammalian expression of hSar1 with C-term V5 and His6 tagDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESUMO-hRORg1
Plasmid#170330PurposeExpresses RORg isoform 1 with N-terminal HisSUMO tag in bacteriaDepositorAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-PCNA-19
Plasmid#54221PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorAvailable SinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAP03-ermE*p
Plasmid#120234PurposeCarries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing ermE*p promoter and ampicillin resistance gene
UseSynthetic BiologyMutationbla (amp resistance gene)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TDsmURFP-RBS-HO-1
Plasmid#80342PurposeExpresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.DepositorInsertsTandem Dimer smURFP
HO-1
Tags6 Histidine TagExpressionBacterialPromoterpBADAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB114
Plasmid#68568PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsGlucocorticoid receptor (GR)ExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP11H
Plasmid#188063PurposePROSS-designed versatile peroxidase 11HDepositorInsertPROSS-designed versatile peroxidase from Pleurotus ostreatus
TagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHLP700
Plasmid#203742PurposeC-terminal tagging of target proteins with AID*/3xHA degronDepositorInsertIAA17 auxin binding domain codons 71-114
UsePcr amplification template for gene taggingTags3xHAPromoternoneAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHLP701
Plasmid#203743PurposeC-terminal tagging of target proteins with 3xV5-IAA17 degronDepositorInsertIAA17 auxin binding domain
UsePcr amplification template for gene taggingTags3xV5PromoternoneAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SAR1-no stop
Plasmid#67345PurposehSar1 without stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4in
Plasmid#176232PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4out
Plasmid#176233PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB33 (pUC19-KANMX-tetO7-CYCpr)
Plasmid#210472PurposePlasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.DepositorInsertKanMX-TetO7-CYCpr cassette
UseSynthetic BiologyAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisense IRES
Plasmid#69910PurposeExpresses a GFP circular RNA containing the antisense EMCV IRES in mammalian cellsDepositorAvailable SinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB405
Plasmid#68670PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1
Plasmid#67472PurposeMammalian expression of hSar1 with stop codon (native expression)DepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB402
Plasmid#68667PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS hIhh (CT#402)
Plasmid#13995DepositorInsertIhh (IHH Human)
ExpressionBacterialAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB435
Plasmid#68700PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS Galectin1
Plasmid#19216DepositorInsertGalectin1 (LGALS1 Chicken)
ExpressionBacterialAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA3-eIFiso4E120(+)
Plasmid#86092PurposeThis vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.DepositorInserteIFiso4E
ExpressionPlantAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRCHisB-TRF2∆M
Plasmid#50489PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 Delta Myb (TERF2 Human)
Tags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-437PromoterpTrcAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGG-GFP-PM-TurboID
Plasmid#209402PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in plantaDepositorInsertcTP-3XHA-TurboID
TagsGFPExpressionPlantPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateMutationThe 3’-end of the chloramphenicol resistance gene…Available SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP5H
Plasmid#188061PurposePROSS-designed versatile peroxidase 5HDepositorInsertPROSS-designed versatile peroxidase from Pleurotus ostreatus
TagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation43 PROSS mutations, described in publicationAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) mPea3 EnR (CT#1120)
Plasmid#13909DepositorInsertPea3 (Etv4 Mouse)
UseRetroviral; Avian expressionAvailable SinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRC1765
Plasmid#141346PurposeMini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB406
Plasmid#68671PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Wnt3a (CT#169)
Plasmid#13976DepositorInsertWnt3a (Wnt3a Mouse)
UseRetroviral; Avian expressionAvailable SinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre_59.F2
Plasmid#60207PurposeExpresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
TagsFKBP12 and Nuclear localisation signalExpressionMammalianMutationamino acids 19-59 of the humanized version of Cre…PromoterCMVAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID
Plasmid#209393PurposeTransiently expressing TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
ExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNAseq_cal_Tuschl
Plasmid#59426PurposeEncodes sequence non-cognate to human genome for generation of RNAseq calibratorsDepositorInsertRNAseq_cal_Tuschl
Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB225
Plasmid#68616PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only