We narrowed to 15,575 results for: NOL
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Plasmid#141049PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF4-2A-EGFP
ExpressionMammalianAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF2-2A-EGFP-L2
Plasmid#141050PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF2-2A-EGFP
ExpressionMammalianAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9-L2
Plasmid#141040PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9
ExpressionMammalianAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-EGFP-PBL-R1
Plasmid#141018PurposeMutisite Gateway mediated vector constructionDepositorInsertEGFP
ExpressionMammalianAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-E2A-cyOFP1
Plasmid#141084Purposeprecursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-E2A-cyOFP1
UseLentiviralPromoterCMVAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-HxHLICNot
Plasmid#140183PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTags12xHisPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP89b CvTA V124N
Plasmid#141207PurposeChromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.DepositorInsertCvTA
ExpressionBacterialMutationV124NAvailable SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-T7-CBD-hGLY
Plasmid#134940PurposeT7 based expression of human glycine acyltransferase with an N-terminal chitin binding domainDepositorInserthuman glycine acyltransferase 2 (GLYATL2 )
Tagschitin binding domainExpressionBacterialPromotert7Available SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-cimA3.7
Plasmid#134595PurposeLow Phosphate inducible mutant citramalate synthaseDepositorInsertcimA3.7
ExpressionBacterialMutationI47V, E114V, H126Q, T204A, L238SPromoteryibDpAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-SBS-mCherry
Plasmid#134598PurposeLow Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)DepositorInsertSBS-mCherry
TagsmCherryExpressionBacterialPromoteryibDpAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-β20cp6
Plasmid#127060PurposeLow phosphate inducible circular permutation of GFP, which is a substrate of the lon proteaseDepositorInsertGFPcp6-beta20
TagsHis tagExpressionBacterialPromoteryibDAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0652
Plasmid#129013PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly_spike-in
Plasmid#125151PurposeVector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cellsDepositorInsertD. pseudoobscura peak #47 enhancer
UseStap-seq screening vectorAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT271.361
Plasmid#127846Purposeclone A3A-W98Y CBEs by SapI Golden GateDepositorInsertA3A-W98Y
MutationW98YAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE420:pNEO_CAC1_Vn_HOG1 terminator
Plasmid#122701PurposePlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus proteinDepositorInsertN-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS-BacA-21222-FLP-3xFLAG
Plasmid#109165PurposeEncodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFlp
Tags3xFLAGExpressionInsectPromoterPolyhedrinAvailable SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE418:pNAT_ACA1_Vc_HOG1 terminator
Plasmid#122700PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus proteinDepositorInsertC-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC111
Plasmid#118906Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6DepositorInsertNcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
TagsLPETGg, HIS6ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω2
Plasmid#101705PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω1
Plasmid#101706PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α2
Plasmid#101707PurposeGoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α1
Plasmid#101708PurposeGoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω2
Plasmid#101709PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15α2
Plasmid#101711PurposeGoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD-MAM33MTS (12)
Plasmid#101693PurposeMAM33MTS in domestication vectorDepositorInsertCCAT-[MTS from S. cerevisiae Superoxide dismutase [Mn] (YHR008C)from S. cerevisiae Mitochondrial acidic protein (YIL070C)]-AATG
UseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_25-eGFP
Plasmid#107413PurposeThis plasmid contain promoter PthrC3_25, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrC3_25Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_7-eGFP
Plasmid#107412PurposeThis plasmid contain promoter PthrC3_7, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrC3_7Available SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6637
Plasmid#106164PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistanceDepositorInsertunknown
ExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)
Plasmid#108239PurposeExpresses PdR-PCNA2(L171C) fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C) fusion protein
TagsHis6 tagExpressionBacterialMutationPCNA2,L171CPromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15T+1X
Plasmid#108188PurposePCNA1-PdX fusion proteinDepositorInsertPCNA1-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationC73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR161Q
Plasmid#92413PurposehQPRTase harboring R161Q mutationDepositorInserthQPRTase (QPRT Human)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationR161QPromoterT7Available SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCML 221
Plasmid#98587PurposepMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).DepositorInsertMS2
PromoterpLacOAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIAhqprtR161G
Plasmid#92412PurposehQPRTase harbouring R161G mutationDepositorInserthQPRTase (QPRT Human)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationR161GPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR102Q
Plasmid#92407PurposehQPRTase harboring R102Q mutationDepositorInserthQPRTase (QPRT Human)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationR102QPromoterT7Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtK171S
Plasmid#92404PurposehQPRTase harbouring K171S mutationDepositorInserthQPRTase (QPRT Human)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationK171SPromoterT7Available SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR138Q
Plasmid#92411PurposehQPRTase harbouring R138Q mutationDepositorInserthQPRTase (QPRT Human)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationR138QPromoterT7Available SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR102A
Plasmid#92405PurposehQPRTASE harbouring R102A mutationDepositorInserthQPRTase (QPRT Human)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationR102APromoterT7Available SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA
Plasmid#87572PurposePV4 gRNA entry vector for CRISPR decoderDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA
Plasmid#89056PurposePV3 gRNA entry vector for CRISPR decoderDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB6
Plasmid#83557PurposeUsed to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.DepositorInsertGAL10 promoter-yEGFP
ExpressionYeastPromoterGAL10Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
const #7-RFP
Plasmid#81081Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23109Available SinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pconst #6-RFP
Plasmid#81080Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23117Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARK1a
Plasmid#81016PurposeE.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-PMKco
PMD-NudB
PromoterLacUV5 and Trc promoterAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-x16
Plasmid#71505PurposeflySTARR-seq screening vector with x16 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectAvailable SinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RpS12-Gateway
Plasmid#71508PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectAvailable SinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-eEF1delta
Plasmid#71501PurposeflySTARR-seq screening vector with eEF1delta core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCEB3B
Plasmid#67117PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 ZFYVE9
Plasmid#67134PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119
Plasmid#66136PurposeExpresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
TagsSulfolobus solfataricus PCNA3ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(T214V)
Plasmid#66137PurposeExpresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
TagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationT214VPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only