We narrowed to 13,780 results for: TIM

1. Cry2(1-531)-mCh-BAXS184E

   Plasmid

   #117238

   Purpose

   Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

   Depositor

   Robert Hughes

   Article

   Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

   Insert

   Cry2531-mCh-BAXS184E
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPIDCB2

   Plasmid

   #169900

   Purpose

   Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

   Depositor

   Richard Schneider

   Article

   Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

   Type

   Empty backbone

   Use

   Avian expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dox inducible

   Available since

   Dec. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYZ173

   Plasmid

   #98410

   Purpose

   Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

   Plasmid

   #20946

   Purpose

   3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   halorhodopsin
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. tetO-Myocd

   Plasmid

   #46033

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   Myocd (Myocd Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEB2-TagRFP-T

   Plasmid

   #103998

   Purpose

   Plasmid encoding TagRFP-T

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   TagRFP-T
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (R32G, K42R, K67R, …

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KA1153_pPBCAG-MerCreMer-IN

   Plasmid

   #124183

   Purpose

   piggyBac-based expression of the tamoxifen-inducible Cre

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   MerCreMer-IRES-Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

   Plasmid

   #174137

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BRAC/pcDNA3

   Plasmid

   #51967

   Purpose

   mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

   Insert

   BRAC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pPIDNB

    Plasmid

    #169901

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Aivian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LAMP-ReNL_pcDNA3

    Plasmid

    #89532

    Purpose

    Red color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 1/8-NORAD-(A)4x

    Plasmid

    #199209

    Purpose

    1/8 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Esther Braselmann

    Article

    Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

    Insert

    1/8-NORAD
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-C1-CAMKIIalpha-K42R

    Plasmid

    #21221

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CAMKIIalpha-K42R (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K42R (Lab plasmid KS 0007).

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP-C1-CaMKIIbeta(K43R)

    Plasmid

    #21225

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CaMKIIbeta(K43R) (Camk2b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CaMKIIbeta(K43R) Lab plasmid CF 0003.

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 1/2-NORAD-(A)4x

    Plasmid

    #199208

    Purpose

    1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Esther Braselmann

    Article

    Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

    Insert

    1/2-NORAD
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mMaroon-PAK-mMaroon

    Plasmid

    #84361

    Purpose

    FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-PAK-mMaroon
    

    Use

    Tags

    mMaroon flanking PAK domain on N and C terminus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAJM.969

    Plasmid

    #108537

    Purpose

    MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    MphRAM + EryR + PMph-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MphR: N94D

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2-QFGal4-SV40pA

    Plasmid

    #155126

    Purpose

    In vitro transcription of QFGal4 mRNA

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4
    

    Use

    Expression in zebrafish

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSETb-ffDronpa

    Plasmid

    #78182

    Purpose

    Expresses ffDronpa E. coli strains

    Depositor

    Peter Dedecker

    Article

    Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

    Insert

    ffDronpa
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpyC-GFP

    Plasmid

    #220191

    Purpose

    sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge