We narrowed to 6,511 results for: itch

1. pLV_ADRB2-mTurquoise2-iLID

   Plasmid

   #161002

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   ADRB2-mTurquoise2-iLID
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBad-LgBiT-PhoCl1-SmBiT-MBP

   Plasmid

   #164034

   Purpose

   luciferase-based PhoCl screening construct

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   LgBiT-PhoCl1-SmBiT-MBP
   

   Use

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBad promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriEx-mCherry-LOV2 V416L

   Plasmid

   #81036

   Purpose

   mammalian expression of LOV V416L, a mutant with super long relaxation time

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV V416L
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   V416L

   Promoter

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ER-mCherry-eMagA

   Plasmid

   #162248

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   CytP450 ER targeting sequencel-mCherry-eMagA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbE5a-Opto-Cre-Vvd

   Plasmid

   #134404

   Purpose

   Light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   Opto-Cre-Vvd
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. eMagA-mCherry-Mito

   Plasmid

   #162251

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagA-mCherry-OMP25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR-mCherry-CRY2-NLS

   Plasmid

   #145259

   Purpose

   Mammalian Expression of Nuclear Control OptoConstruct with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBLADE_ONLY_C

   Plasmid

   #168050

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Insert

   Blue light-inducible AraC dimers in Escherichia coli
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101**

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3-LOV-VP16

   Plasmid

   #42499

   Purpose

   LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

   Depositor

   Ricardo Dolmetsch

   Article

   Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

   Insert

   FKF1 (N-LOV, G128D mutant) fused with VP16
   

   Use

   Tags

   F-type VP16 activation domain and HA tag

   Expression

   Mammalian

   Mutation

   G128D 1-168aa

   Promoter

   CMV

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-N-I-N-CAAX

    Plasmid

    #41686

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145N-IntersectinDH-Dronpa145N-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-b2AR-eLOV-TEVcs-FLAG-GAL4-V5

    Plasmid

    #104841

    Purpose

    b2AR-SPARK TF component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    b2AR-eLOV-TEVcs-FLAG-GAL4-V5
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBMN HAYFP-LOV24

    Plasmid

    #49570

    Purpose

    regulate protein stability with light

    Depositor

    Thomas Wandless

    Article

    Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

    Insert

    YFP-LOV24
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    delete the last 3 aa from AsLOV2, with I532A muta…

    Promoter

    LTR

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ca-FLARE (TF)

    Plasmid

    #92213

    Purpose

    Transcription factor component of FLARE

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-NBid-PhoCl2c-CBid

    Plasmid

    #164051

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl2c

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl2c-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDS221

    Plasmid

    #34981

    Purpose

    Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb1–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTriEx-mCherry-LOV2 I427T

    Plasmid

    #81035

    Purpose

    Mammalian expression of LOV I427T, a mutant with super short relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV I427T
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    I427T

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGal4AD-CIBN

    Plasmid

    #28246

    Purpose

    Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1 (1-171aa)
    

    Use

    Tags

    Gal4AD

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NLSx3-CRY2PHR-p65

    Plasmid

    #64124

    Purpose

    Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    CRY2PHR-p65
    

    Use

    Tags

    His, NLSx3, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Fus-BcLOV-mCh-SOS

    Plasmid

    #208275

    Purpose

    Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    Fus-BcLOV-mCh-SOS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSP64-GFP-ePDZ-CAAX

    Plasmid

    #135603

    Purpose

    Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    Lifeact-GFP-ePDZ-CAAX
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    CAAX, EGFP, and ePDZ

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAGGS-SspB-mScarlet-I

    Plasmid

    #178521

    Purpose

    Expresses SspB-mScarlet-I in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    SspB-mScarlet-I
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TagRFP-T-VAPB(1- 218)- eMagB

    Plasmid

    #162255

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    TagRFP-T-VAPB(1- 218)- eMagB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Plasma membrane-targeted optodPLD

    Plasmid

    #140061

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT2ADWpuro_2paRAF

    Plasmid

    #129653

    Purpose

    For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PhyB-mCherry-Tom7

    Plasmid

    #66571

    Purpose

    Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae

    Depositor

    Orion Weiner

    Article

    Jost et al ACS Synth Biol. 2015 Jun 2.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    Tom7 and mCherry

    Expression

    Yeast

    Mutation

    PhyB portion contains aa's 1-908

    Promoter

    pADH1

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. His6_BcLOV4_mCherry_BamUK

    Plasmid

    #114596

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Use

    Tags

    His6 and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCBI-LexA-CIB1-biLINuS

    Plasmid

    #192605

    Purpose

    Plasmid for constitutive expression of LexA-CIB1-biLINuS.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    LexA-CIB1-biLINuS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-K-I-N-CAAX

    Plasmid

    #41640

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145K-IntersectinDH-Dronpa145N-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLIC-lyn11-myc-LOV2GIVe

    Plasmid

    #170623

    Purpose

    For the mammalian expression of mLOV2GIVe

    Depositor

    Mikel Garcia-Marcos

    Article

    Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

    Insert

    Lyn11-myc-LOV2GIVe
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pBabe-PI(WT)-Src(WT)-mCherry

    Plasmid

    #87356

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDS257

    Plasmid

    #34971

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV_Lyn11-mTurquoise2-iLID

    Plasmid

    #161000

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    Lyn11-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

    Plasmid

    #202719

    Purpose

    Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

    Depositor

    Adam Cohen

    Article

    Xiang et al bioRxiv 2023

    Inserts

    Myr-Pink Flamindo
    EGFP-bPAC-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-PhoCl-mCherry-myc

    Plasmid

    #87692

    Purpose

    Express PhoCl-mCherry with C-term myc tag in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl-mCherry-myc
    

    Use

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV_mTurquoise2-iLID-CAAX

    Plasmid

    #160999

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    mTurquoise2-iLID-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTriEx-mCherry-LOVSD

    Plasmid

    #81033

    Purpose

    Mammalian expression of the super-dark state of LOV2

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOVSD
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    C450A, L514K, G528A, L531E, N538E

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4

    Plasmid

    #135460

    Purpose

    Mammalian expression of uFLARE TF component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
    

    Use

    Tags

    HA, flag,

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mCherry-PI(WT)-Vav2(DPZ)

    Plasmid

    #86974

    Purpose

    PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/C1 (Vav2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge