We narrowed to 12,214 results for: LEA
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Plasmid#74110PurposePlasmid containing tetracycline resistance gene cassette flanked by multiple restriction sitesDepositorInserttetA(C)
UseBacterial vector based on pmb1 repliconMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…Available sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ220-Ultra
Plasmid#176889PurposeGateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoterDepositorInsertAsCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNucleoplasmin NLS and SV40 NLSExpressionPlantAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
Tags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIB2
Plasmid#112700PurposeExpression of NFIB2 protein and GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNuclear Factor I B2 (Nfib Mouse)
TagsGFP and HAExpressionMammalianAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY006 (pcDNA3.1-hBpCpf1)
Plasmid#69978PurposeExpresses humanized BpCpf1DepositorInserthBpCpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.BB.3z
Plasmid#200679PurposeModular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-miRFP670nano-miRFP670
Plasmid#216131PurposeLentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.DepositorInsertEF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
UseLentiviralExpressionMammalianAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-HiFi SpCas9-NLS-6xHis
Plasmid#207376PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OT1.0
Plasmid#185387PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin sensor GRAB_OT1.0
UseAAVPromoterEFSAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-mono-MAD7
Plasmid#170134PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRExpressionPlantPromotermaize Ubiquitin-1, switchgrass Ubiquitin-1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP_P2A_Halo_GluA2
Plasmid#170454PurposeExpresses halo tagged GluA2 in neuronal cellsDepositorPublicationAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-TP1107(Q15TAG)
Plasmid#247462PurposeBacterial expression of anti-IgG VHH clone TP1107 with amber stop codon at Q15 for unnatural amino acid incorporationDepositorInsertTP1107 Q15TAG
TagsHis6 and TEV protease cleavage siteExpressionBacterialMutationOriginal Q15 codon (CAA) was modified to amber st…PromoterT7Available sinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE
Plasmid#89587PurposeFor nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnuclear localization signal (nls) turboRFP
ExpressionMammalianAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGem-sHyper:sAPX
Plasmid#84738PurposeSimultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cellsDepositorInsertsTagsnuclear localisation signals from the coding sequ…ExpressionPlantPromoterpFMV/p35SAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
TagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-4GS-LaG-2
Plasmid#172764PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)DepositorInsertLaG-16-4GS-LaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-NRBF2
Plasmid#99330PurposeMammalian expression of NRBF2, which associates with the PI3KC3-C1 complexDepositorInsertNuclear receptor-binding factor 2 (NRBF2 Human)
TagsHis-MBPExpressionBacterialPromoterT7Available sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpRY-ABE8e(V106W)
Plasmid#198553PurposeExpresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpRY-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpRY-YE1-BE4max
Plasmid#198554PurposeExpresses human codon-optimized SpRY-YE1-BE4max and blasticidin resistance: EFS promoter-SpRY-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpRY-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only