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Plasmid#83310PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-R10
UseTags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204C/F223R/S202K/K41DPromoterT7Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-pcDronpa2-A69T
Plasmid#98578Purposephotoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpressionDepositorInsertpcDronpa2-A69T
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS316-GFP-COF1
Plasmid#31843DepositorInsertCofilin (COF1 Budding Yeast)
UseTagsGFP (S65T)ExpressionYeastMutationPromoterT7Available sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET-A2-77p-Venus
Plasmid#64856PurposeExpresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tagDepositorInsertVWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)
UseTags7X His and VenusExpressionBacterialMutationPromoterT7 PromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mKikGR-V69T
Plasmid#98574Purposephotoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmKikGR-V69T
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3
Plasmid#79613PurposeExpresses dCas9-SH3 in yeast cellsDepositorInsertSpCas9
UseTagsSH3 domainExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis
Plasmid#60390PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4)PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM2-mRUBY2
Plasmid#107793Purposedonor vector for knock-in gene editing at human DNM2 locusDepositorInsertDNM2-mRuby2 (DNM2 Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM1-EGFP
Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1
Plasmid#18684DepositorInsertRnq1 (RNQ1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
7859 pRS305-pUG72
Plasmid#10897DepositorInsertUra3 (URA3 Budding Yeast)
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS403-PGAL1-weakSC_GFP
Plasmid#22315DepositorInsertGFP-rad9
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPmal-25u
Plasmid#14480DepositorInsertFLIPmal
UseTagsECFP and EYFPExpressionBacterialMutationW230APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_PCP-VP64_B-PP7
Plasmid#177157PurposeExpresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cellsDepositorInsertsPCP-VP64
B-PP7
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSAN-QUEEN 84BP
Plasmid#129378Purposea variation of QUEEN ATP sensorDepositorInsertQUEEN TogBP (84BP) N/A
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Num1-PH
Plasmid#71374PurposeFor analysis of PH domain localization in yeastDepositorInsertNum1-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa2563-2692PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) pRSETa
Plasmid#8554DepositorInsertHOXD12 (Hoxd12 Mouse)
UseTagsHIS and XPressExpressionBacterialMutationFusion sequence is: ggatccATGPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mm
Plasmid#51059PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GST-05230
Plasmid#67874PurposeE.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.DepositorInsertshy1
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-spa-GCaMP6f
Plasmid#67563Purposebacterial expression of a photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseTagsHIS-tagExpressionBacterialMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE-100u
Plasmid#13539DepositorInsertFLIP-E S118W
UseTagsECFP and VenusExpressionBacterialMutationS118W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII32Pglu-2.2m
Plasmid#13566DepositorInsertFLIPglu F16A P32S-ECFP-D33R
UseTags6xHis and CitrineExpressionBacterialMutationF16A, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1(406)
Plasmid#122068PurposeExpressing ATG1DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS4-blast
Plasmid#158473PurposeConstitutive lentiviral expression of human TMPRSS4.DepositorInsertTMPRSS4 (TMPRSS4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII12Pglu-600uDelta4
Plasmid#13567DepositorInsertFLIPglu F16A Y12S-ECFP-D13R, 4aa linker
UseTagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBPromoterAvailable sinceFeb. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-1m
Plasmid#13551DepositorInsertFLIP-E intramol A207W (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationA207W, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPmal-225u
Plasmid#14481DepositorInsertFLIPmal
UseTagsECFP and EYFPExpressionBacterialMutationW62APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSV-p65T305A,S276A
Plasmid#106456PurposeNF-kB p65, kinase sites 276,305 mutatedDepositorInsertNF-kB p65, kinase sites 276,305 mutated (RELA Human)
UseTagsExpressionMammalianMutationkinase sites inactivatedPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS414 GPD-Sis1deltaG/F
Plasmid#18688DepositorInsertSis1 deltaG/F
UseTagsExpressionYeastMutationSis1 with the G/F region deletedPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 (1-428)
Plasmid#60395PurposeExpression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationdeleted 429 - endPromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-Tmprss6(S762A)
Plasmid#18791DepositorInsertType II transmembrane serine protease 6 (Tmprss6 Mouse)
UseTags3xFLAGExpressionMammalianMutationChanged Serine762 to AlaninePromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-LSS-mEGFP
Plasmid#112942PurposeBacterial expression vector for LSS-EGFPDepositorInsertLSSS-EGFP
UseTagsExpressionMammalianMutationPromoterT7Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-LSS-SGFP2
Plasmid#112939PurposeBacterial expression vector for LSS-SGFP2DepositorInsertLSS-SGFP2
UseTagsExpressionMammalianMutationPromoterT7Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPmal-control
Plasmid#14482DepositorInsertFLIPmal
UseTagsECFP and EYFPExpressionBacterialMutationW340APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(E224Q)
Plasmid#32027DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationE224QPromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-RoTq-Off
Plasmid#234466PurposeBacterial expression vector for RoTq-OffDepositorInsertRoTq-Off
UseTags7xHisExpressionBacterialMutationPromoterAvailable sinceMay 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN-pRSFDuet
Plasmid#238342Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta(wt) in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (wt) (dnaN E. coli)
UseTags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationnone (wt)PromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-CFAPorig
Plasmid#221092PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 S1584F
Plasmid#234122PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationS1584FPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 S1584Y
Plasmid#234121PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationS1584YPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 V1862L
Plasmid#234123PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationV1862LPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Fyv6-CFLAG
Plasmid#234133PurposeRestriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-NFLAG-Fyv6
Plasmid#234139PurposeRestriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 G1868R
Plasmid#234124PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationG1868RPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-M175I
Plasmid#234118PurposeSuppressor screen mutant in Cef2DepositorInsertCef1
UseTagsExpressionYeastMutationM175IPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-Q193P
Plasmid#234117PurposeSuppressor screen mutant in Cef1DepositorInsertCef1
UseTagsExpressionYeastMutationQ193PPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-A37V
Plasmid#234119PurposeSuppressor screen mutant in Cef3DepositorInsertCef1
UseTagsExpressionYeastMutationA37VPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 WT TRP1
Plasmid#234120PurposeThe N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORFDepositorInsertPrp8
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-CUP1pr-NFAPoptim
Plasmid#221110PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-CUP1pr-CFAPoptim
Plasmid#221111PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only