Addgene

We narrowed to 4,261 results for: TIL

Showing: 651 - 700 of 4261 results

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pDM291-tetR'-tup11Δ70

Plasmid

#41028

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetR'-tup11Δ70

Use

Tags

Expression

Yeast

Mutation

tup11 starts at aa70

Promoter

Available since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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p7XC3H

Plasmid

#47065

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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LSL

Plasmid

#66590

Purpose

Lox-STOP-Lox reporter

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGGC011

Plasmid

#48825

Purpose

Provides alcR as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

alcR

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCM130

Plasmid

#45828

Depositor

Mary Lidstrom

Article

Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

Type

Empty backbone

Use

Low-background broad-host-range promoter-probe ve…

Tags

Expression

Mutation

Promoter

none

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pEP-EGFPin

Plasmid

#60961

Purpose

PCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

eGFP-Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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iGFP

Plasmid

#66589

Purpose

inverted GFP reporter

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

GFP

Use

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA_Level 1A

Plasmid

#102701

Purpose

Mobius Assembly Level 1 Acceptor Vector A

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:spisPink:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGD000

Plasmid

#48859

Purpose

Empty entry vector for creating GreenGate C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC026

Plasmid

#48831

Purpose

Provides 3xmCherry as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

3xmCherry

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGD001

Plasmid

#48833

Purpose

Provides linker:GFP as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

Linker:GFP

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Use

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Tg(krt4:EGFP-Mmu.Rpl10a)

Plasmid

#128839

Purpose

RNA isolation from keratinocytes via TRAP

Depositor

Anna Huttenlocher

Article

Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

Insert

EGFP-L10a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGG002

Plasmid

#48851

Purpose

Provides an H-A adapter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

H-A adapter - short

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMyBADNT

Plasmid

#84690

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

AraC

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGGG001

Plasmid

#48850

Purpose

Provides an F-H adapter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

F-H adapter - short

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGB001

Plasmid

#48819

Purpose

Provides mCherry:linker as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

mCherry:Linker

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGB000

Plasmid

#48857

Purpose

Empty entry vector for creating GreenGate N-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1107_Tier3(SB)

Plasmid

#169634

Purpose

Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase (5'ITR-A1-pA::A2-pA::A3-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXC3H

Plasmid

#47068

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN cFKT

Plasmid

#18785

Depositor

Han Htun

Article

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Insert

TRAP (mtrB Bacillus subtilis)

Use

Tags

6xHis and HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1048

Plasmid

#208738

Purpose

Characterization plasmid for PRelA (sfgfp expressed from PRelA on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PRelA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PRelA

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA_Level 2A

Plasmid

#102705

Purpose

Mobius Assembly Level 2 Acceptor Vector A

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:sfGFP:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p7XC3GH

Plasmid

#47066

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGM000

Plasmid

#48864

Purpose

Empty entry vector for creating GreenGate supermodules w/o plant resistance cassette.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGN000

Plasmid

#48865

Purpose

Empty entry vector for creating GreenGate supermodules with plant resistance cassette.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC012

Plasmid

#48826

Purpose

Provides GFP:NLS as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP:NLS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGD005

Plasmid

#48853

Purpose

Provides linker:BFP as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

Linker:BFP

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE000

Plasmid

#48860

Purpose

Empty entry vector for creating GreenGate terminator modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXNH3

Plasmid

#47067

Purpose

FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
EYK054

Plasmid

#69512

Purpose

pBAD pduP1-18-gfpmut2-ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

GFPmut2

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPHLWL

Plasmid

#26254

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Multiple cloning site

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA_Level 1Δ

Plasmid

#102704

Purpose

Mobius Assembly Level 1 Acceptor Vector Δ

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:spisPink:rrnBT1-T7TE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CMJ119

Plasmid

#69517

Purpose

pBAD eutE1-20- gfpmut2-ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

GFPmut2

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a- mEGFP-kanMX6

Plasmid

#105146

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mEGFP-kanMX6

Use

Tags

mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pXN-FBLWLF

Plasmid

#26262

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Multiple cloning site

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK-FLARE16-S aadA-GFP

Plasmid

#123562

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

FLARE16-S aadA-GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK_proJK1_LGK

Plasmid

#81160

Purpose

Expresses levoglucosan kinase with promoter proJK1

Depositor

Timothy Whitehead

Article

Klesmith et al ACS Synth Biol. 2015 Sep 14.

Insert

Levoglucosan kinase wild-type

Use

Tags

His6x

Expression

Bacterial

Mutation

Promoter

proJK1

Available since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC051

Plasmid

#48832

Purpose

Provides GUS as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GUS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC015

Plasmid

#48828

Purpose

Provides mCherry as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

mCherry

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTLNXA7

Plasmid

#49033

Purpose

FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Xenopus oocytes expression

Tags

chicken alpha7 signal sequence

Expression

Mutation

Promoter

SP6

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_Strep-Psc-pScaC

Plasmid

#228834

Purpose

E. coli expression of the ScaC passenger domain

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Use

Tags

Strep

Expression

Bacterial

Mutation

aa 33-223

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_pScaC(50-159)

Plasmid

#228836

Purpose

E. coli expression of a truncated ScaC passenger domain (aa 50-159)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

ScaC

Use

Tags

Strep

Expression

Bacterial

Mutation

aa 50-159

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
INT_SS9sp

Plasmid

#212145

Purpose

Effector plasmid for for operon integration into the genome (Safe Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

Depositor

Kang Zhou

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC

Use

Tags

Expression

Bacterial

Mutation

Promoter

pJ23119

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1051

Plasmid

#208739

Purpose

Characterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PZwf

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PZwf

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1054

Plasmid

#208740

Purpose

Characterization plasmid for PAmy (sfgfp expressed from PAmy on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PAmy

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PAmy

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1055

Plasmid

#208741

Purpose

Characterization plasmid for PHrcA (sfgfp expressed from PHrcA on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PHrcA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PHrcA

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR1057

Plasmid

#208742

Purpose

Characterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PPgk

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PPgk

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK-E.coli rrnB 3'UTR

Plasmid

#123573

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

E.coli rrnB 3'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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