We narrowed to 1,130 results for: ncl

1. pSpCas9_U6_sgRNA_Mettl3C

   Plasmid

   #165420

   Purpose

   sgRNA construct for targeting Mettl3 C-terminus

   Depositor

   Neil Brockdorff

   Article

   Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

   Insert

   PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-hGABARAP

   Plasmid

   #87871

   Purpose

   Express a EGFP-human GABARAP in mammalian cells

   Depositor

   Isei Tanida, Eiki Kominami

   Article

   Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

   Insert

   GABARAP (GABARAP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3-HA-Akt1-K179M

   Plasmid

   #73409

   Purpose

   Expresses kinase-inactive mutant of Akt with HA tag

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   changed K179M to make Kinase inactive mutant

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p6345 MSCV-CMV-Flag-HA-Brd4 1-722

   Plasmid

   #31352

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   Brd4 1-722 (BRD4 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   *see comments

   Promoter

   Available since

   Oct. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA3-HA-Akt1-aa1-149

   Plasmid

   #73410

   Purpose

   Expresses HA tagged aa1-149 of AKT1

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Expresses aa 1-149 only of AKT1

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3-HA-Akt1-aa120-433

   Plasmid

   #73411

   Purpose

   Expresses HA tagged aa120-433 AKT1

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Expresses aa 120-433 of AKT1

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET21b-Caspase-3(D3A: D175A D28A D9A)

   Plasmid

   #90090

   Purpose

   Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

   Depositor

   Clay Clark

   Article

   Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D175A D28A D9A

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-4mtD3cpv

   Plasmid

   #181874

   Purpose

   Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   4mtD3cpv
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   the 4mtD3cpv CDS differs from the recorded GeneBa…

   Promoter

   CaMKIIa

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3-HA-Akt1-aa1-408

   Plasmid

   #73412

   Purpose

   Expresses HA tagged aa1-408 of AKT1

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Expresses aa 1-408 of AKT1

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pENTR_Mm_CEP55(WT)

    Plasmid

    #136333

    Purpose

    Mouse CEP55 cDNA in Gateway pENTR

    Depositor

    Marcos Malumbres, Guillermo de Cárcer

    Article

    de Carcer et al Nat Commun. 2018 Aug 1;9(1):3012. doi: 1

    Insert

    CEP55 (Cep55 Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3 T7 DLC1

    Plasmid

    #24266

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    DLC1 (DYNLL1, DLC1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pHH0103_DYNLL1_Dynein-light

    Plasmid

    #110006

    Purpose

    Protein expression and purification of DYNLL1_Dynein-light

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    DYNLL1_Dynein-light (DYNLL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EGFP-CERT-PH

    Plasmid

    #49572

    Purpose

    expresses the plekstrin homology domain of CERT/OSBP in mammalian cells

    Depositor

    Marci Scidmore

    Article

    Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

    Insert

    GPBP (CERT1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expresses AA 1-122

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET-6xHis-GFP(+36)-DL4

    Plasmid

    #199168

    Purpose

    used to express a supercharged GFP variant that forms inclusion bodies in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+36)-DL4
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

    Promoter

    T7

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362

    Plasmid

    #31354

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 1047-1362 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLEX-Frb:Akt2-IRES-Hygromycin

    Plasmid

    #120713

    Purpose

    Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    AKT2 (AKT2 Human)
    

    Use

    Lentiviral

    Tags

    FRB(T2098L)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p6347 MSCV-CMV-Flag-HA-Brd4-444-722

    Plasmid

    #31353

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 444-722 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEMS1725

    Plasmid

    #105870

    Purpose

    High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    iCreERT2-FFrt-KanR-FFrt
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p6349 MSCV-CMV-Flag-HA-Brd4 1224-1362

    Plasmid

    #31355

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 1224-1362 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRLCon1-3180

    Plasmid

    #31445

    Depositor

    Gerhart Ryffel

    Article

    Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

    Insert

    HNF4A 3'UTR (long) (HNF4A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge