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Plasmid#117046PurposeOptimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoterDepositorInsertGFP
UseTransposonPromoterCMVAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)
Plasmid#153005PurposeExpresses high-affinity split-TurboID C-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (C) (G79)
UseLentiviralTagsFRB, HA, and HaloTagExpressionMammalianPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1- dCas9-2xNLS-EGFP
Plasmid#74710PurposeExpresses dCas9-2xNLS-EGFP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-2xNLS-EGFP
UseCRISPRTagsEGFPExpressionMammalianPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TAC-ERP2
Plasmid#80478PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX304 CMV FKBP-V5-sTurboID-high affinity (N)
Plasmid#153004PurposeExpresses high-affinity split-TurboID N-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (N) (G78)
UseLentiviralTagsFKBP and V5ExpressionMammalianPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-UniSAM
Plasmid#99866PurposeEncodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUniSAM-mCherry + U6-gRNA2.0
UseCRISPR and Synthetic Biology; Dcas9-sam activationExpressionMammalianMutationBbsI sites in CDS were ablated by consensus mutat…PromoterEF1aAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS6-YFP-SMASh
Plasmid#68853PurposeExpresses SMASh tag fused to YFP at C terminus in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYFP-SMASh
TagsSMASh tagExpressionMammalianPromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-MmPeg10rc4
Plasmid#174858PurposeExpresses recoded mouse Peg10 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrecoded Mus musculus PEG10 (Peg10 Mouse)
TagsHAExpressionMammalianPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEZ-Frt-lox-DT
Plasmid#11736PurposeConditional gene targeting vector; contain cloning sites for 5′ and 3′ homology arms, and a central targeting region flanked by LoxP sites, and a neomycin cassette flanked by FRT sites.DepositorInsertpEZ-Frt-lox-DT
UseCre/Lox; /frtAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-SynA
Plasmid#174860PurposeExpresses mouse syncytin A in mammalian cellsDepositorAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-mDlx-ChR2-mCherry-Fishell-3
Plasmid#83898PurposeChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas serviceAAV1, AAV9, and AAV RetrogradeInsertChR2
UseAAVExpressionMammalianPromotermDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)
Plasmid#139998PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMVTNT PINK1 C-myc
Plasmid#13314DepositorAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBbi-Hyg
Plasmid#60524PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)
Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLBH531_MBP-Cas14a1 expression
Plasmid#112500PurposeExpresses MBP fused to Cas14a1DepositorInsertCas14a1
UseCRISPRTags10xHis-MBPExpressionBacterialPromoterT7Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
TagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Dendra2-Lifeact-7
Plasmid#54694PurposeLocalization: Actin, Excitation: 490 / 553, Emission: 507 / 573DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeact-Dendra2
ExpressionMammalianAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL1521 (pSPIN, pSC101* backbone)
Plasmid#160729PurposeSingle-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by