We narrowed to 9,185 results for: Pol;

1. Akaluc-N1

   Plasmid

   #120371

   Purpose

   Akaluc luciferase mammalian expression in -N1 backbone

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   Akaluc (human optimized)
   

   Expression

   Mammalian

   Mutation

   Human codon optimized

   Promoter

   CMV

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDP20

   Plasmid

   #171097

   Purpose

   Expresses mAID-tagged mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   mCherry2
   

   Use

   Synthetic Biology

   Tags

   miniAID

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-NP-B/BRISBANE/60/2008

   Plasmid

   #187921

   Purpose

   Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   Influenza B Nucleoprotein
   

   Tags

   UNTAGGED

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZH61 pLIB-8His3C-spt4

   Plasmid

   #231649

   Purpose

   express S.cerevisiae spt4 in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   spt4 (SPT4 Budding Yeast)
   

   Tags

   8His-HRV3C

   Expression

   Insect

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCk2_spacer

   Plasmid

   #136072

   Purpose

   Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. EGFP-Ki67-mCherry

   Plasmid

   #183748

   Purpose

   Expresses EGFP-Ki67-mCherry

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Tags

   Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-ReNL

   Plasmid

   #123138

   Purpose

   Piggybac transposon plasmid for live animal optical imaging of Red-enhanced nanolantern

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   Red-enhanced Nanolantern
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWZL-Blast-Flag-HA-hTERT

   Plasmid

   #22396

   Depositor

   William Hahn

   Article

   Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

   Insert

   TERT (TERT Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-ConVERGD-stGtACR1(FusionRed)-W3SL

   Plasmid

   #218761

   Purpose

   Provides AND intersectional (Cre+Flp) expression of stGtACR1-FusionRed

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   stGtACR1
   

   Use

   AAV

   Tags

   FusionRed

   Promoter

   hSyn

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. EpsilonCOP EGFP

    Plasmid

    #128151

    Purpose

    Expression of epsilon COP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    epsilon COP (COPE Bovine)
    

    Expression

    Mammalian

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. NylCp2-TS_pET28a

    Plasmid

    #215421

    Purpose

    Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCp2-TS
    

    Expression

    Bacterial

    Mutation

    D122G/H130Y/D36A/E263Q

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-NP-V5-B/BRISBANE/60/2008

    Plasmid

    #187923

    Purpose

    Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. TLCV2-sgHPDL #3

    Plasmid

    #174165

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. TLCV2-sgHPDL #9

    Plasmid

    #174166

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G03 CYP2D6

    Plasmid

    #67090

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP2D6 (CYP2D6 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Sankar Adhya, Francisco Malagon

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEx-deletion-erm

    Plasmid

    #172213

    Purpose

    For allelic exchange in erythromycin-sensitive Prevotella copri strains

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Allelic exchange in prevotella copri

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AzoRS-4

    Plasmid

    #182884

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Israeli et al Adv. Funct. Mater. 2021, 31, 2011276

    Insert

    AzoRS-4
    

    Expression

    Bacterial

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK-myc-Par3b

    Plasmid

    #19388

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

    Insert

    Partitioning-defective protein 3b (PARD3 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Full length, isoform b.

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CAG-GFPd2

    Plasmid

    #115665

    Purpose

    Piggybac transposon plasmid for destabilized eGFP (GFPd2)

    Depositor

    Jordan Green

    Article

    Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

    Insert

    GFPd2
    

    Use

    Synthetic Biology

    Tags

    Destabilization

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFW6000

    Plasmid

    #224215

    Purpose

    Bacteroides genomic editing tool; aTc inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCellFree_G03 CYP3A4

    Plasmid

    #67104

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP3A4 (CYP3A4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3.1 human cofilin WT

    Plasmid

    #50853

    Purpose

    expresses human cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pmGFP-P2A-K(AAG)20-P2A-RFP

    Plasmid

    #105689

    Purpose

    Double fluorescent stalling reporter K(AAG)20

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAG)20
    P2A-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV (from vector)

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFPC1-6XHis-FLKSRP

    Plasmid

    #23001

    Depositor

    Douglas Black

    Article

    Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

    Insert

    KSRP (KHSRP Human)
    

    Tags

    EGFP and His

    Expression

    Mammalian

    Mutation

    valine inserted at aa#2

    Available Since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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26. HDM_RSV_A_1982_F

    Plasmid

    #237355

    Purpose

    Expression plasmid for RSV A F from 1982

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV A F from 1982 human codon optimized from Genbank Accesion KJ723472
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. HDM_RSV_A_2020_F

    Plasmid

    #237356

    Purpose

    Expression plasmid for RSV A F from 2020

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV A F from 2020 human codon optimized from Genbank Accesion PP495954
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEGFP-mNFM

    Plasmid

    #32909

    Purpose

    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein M

    Depositor

    Anthony Brown

    Article

    Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

    Insert

    EGFP-mouse NFM fusion (Nefm Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

    Plasmid

    #199442

    Purpose

    CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    HITI donor template
    

    Use

    CRISPR

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. NylCK_pET28a

    Plasmid

    #215422

    Purpose

    Expression construct for NylCK gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCK
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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