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Showing: 6951 - 7000 of 14587 results

  1. cTRE-GFP-miR30_shPten.1523

    Plasmid
    #135667
    Purpose
    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    shPten
    Use
    Mouse Targeting
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135667-plasmid-map-sequence-id-325441

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  4. pREP4xV5_ccdB

    Plasmid
    #79016
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79016-plasmid-map-sequence-id-147773

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  5. STK080_pSTK-0D-bkdB Cold double terminator

    Plasmid
    #235414
    Purpose
    Very strong terminator in B. subtilis. Transcriptional terminator and 3'-mRNA stabilizer from bkdB gene of B. subtilis. Positive impact in expression at 18C. Contain STOP codons in both directions
    Depositor
    Tom Ellis
    Insert
    bkdB Cold double terminator
    Expression
    Bacterial
    Mutation
    No
    Available Since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    235414-plasmid-map-sequence-id-502668

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  6. pTet_KaeCanABC(G189K)

    Plasmid
    #248957
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G189K)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanABC(G189K)
    Expression
    Bacterial
    Mutation
    G189K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248957-plasmid-map-sequence-id-500783

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  7. pTet_KaeCanA(C187A)BC

    Plasmid
    #248954
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with CanA mutated (C187A)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanA(C187A)BC
    Expression
    Bacterial
    Mutation
    C187A
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248954-plasmid-map-sequence-id-500775

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  8. pTet_KaeCanABC(G104K)

    Plasmid
    #248956
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G104K)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanABC(G104K)
    Expression
    Bacterial
    Mutation
    G104K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248956-plasmid-map-sequence-id-500766

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  9. pTet_PflHamMA(deltaA)B

    Plasmid
    #248938
    Purpose
    Hachiman from P. fluorescens under pTet control with the dHamA domain removed
    Depositor
    Jennifer Doudna
    Insert
    PflHamMA(deltaA)B
    Expression
    Bacterial
    Mutation
    HamA domain deletion
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248938-plasmid-map-sequence-id-500763

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  10. pTet_KaeCanABC(H271A)

    Plasmid
    #248955
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (H271A)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanABC(H271A)
    Expression
    Bacterial
    Mutation
    H271A
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248955-plasmid-map-sequence-id-500762

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  11. pDest_PANCEd_AraC_S170_LOV_sd2_R2_C1

    Plasmid
    #242152
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE cassette with a LOV domain insertion at S170
    Depositor
    Dominik Niopek
    Insert
    AraC R2 S170 LOV
    Expression
    Bacterial
    Mutation
    E3K, R146L, A152T, R251L
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242152-plasmid-map-sequence-id-484660

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  12. pDest_PANCEd_AraC_S170_LOV_sd2_R5_C1

    Plasmid
    #242153
    Purpose
    Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE cassette with a LOV domain insertion at S170
    Depositor
    Dominik Niopek
    Insert
    AraC R5 S170 LOV
    Expression
    Bacterial
    Mutation
    A2E, L156I, R251H
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242153-plasmid-map-sequence-id-484652

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  13. pHflu3

    Plasmid
    #203881
    Purpose
    pHI1 with insertion of kanamycin-resistance gene
    Depositor
    Bogumil Karas
    Insert
    aminoglycoside O-phosphotransferase APH(3')-IIa
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203881-plasmid-map-sequence-id-403829

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  14. pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO

    Plasmid
    #204079
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    TALEsp1-stabilized promoter driving lacI repressor; Ptac-lacO IPTG-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204079-plasmid-map-sequence-id-413894

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  15. AAV-CAG-FLEX-LifeAct-jGCaMP8s-WPRE

    Plasmid
    #186049
    Purpose
    AAV-mediated expression of LifeAct-jGCaMP8s under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    LifeAct-jGCaMP8s
    Use
    AAV
    Tags
    LifeAct-6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186049-plasmid-map-sequence-id-370172

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  20. pCASCADE-gapAP1

    Plasmid
    #87146
    Purpose
    gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87146-plasmid-map-sequence-id-174938

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  21. pREP41xV5_ccdB

    Plasmid
    #79017
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79017-plasmid-map-sequence-id-151963

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  22. pREP42xV5_ccdB

    Plasmid
    #79018
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79018-plasmid-map-sequence-id-147775

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  23. pHL 1904

    Plasmid
    #53021
    Purpose
    AmpR + fimS::gfpLVA::Asp terminator + CamR + ColE1
    Depositor
    Han Lim
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Asp terminator, LVA, and fimS
    Expression
    Bacterial
    Promoter
    fimS
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. pLIX_403

    Plasmid
    #41395
    Purpose
    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Tags
    V5
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41395-plasmid-map-sequence-id-364439

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  25. pCW57.1

    Plasmid
    #41393
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available Since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41393-plasmid-map-sequence-id-364440

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  26. pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

    Plasmid
    #128450
    Purpose
    lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128450-plasmid-map-sequence-id-256464

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  27. pCWU50

    Plasmid
    #232318
    Purpose
    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Promoter
    fdx
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232318-plasmid-map-sequence-id-462281

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  28. pRL528

    Plasmid
    #58495
    Purpose
    helper plasmid for bacterial conjugal DNA transfer
    Depositor
    Peter Wolk
    Article
    Elhai et al Methods Enzymol. 1988;167:747-54.
    Insert
    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    Available Since
    Oct. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    58495-plasmid-map-sequence-id-107317

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  29. pMH-Halo tag

    Plasmid
    #154144
    Purpose
    Gateway compatible mammalian expression plasmid for halo tag fusion proteins
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    Halo tag
    Expression
    Mammalian
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154144-plasmid-map-sequence-id-309445

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  33. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Karl Kramer
    Type
    Empty backbone
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53581-plasmid-map-sequence-id-242038

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  34. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

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  35. pZP06

    Plasmid
    #232320
    Purpose
    pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232320-plasmid-map-sequence-id-462279

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  36. pMH-EBFP

    Plasmid
    #161714
    Purpose
    Gateway-compatible destination vector for mammalian expression of N-terminal EBFP protein under CMV promoter
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    cmv
    Available Since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    161714-plasmid-map-sequence-id-319502

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  37. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid
    #218193
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF
    Depositor
    Florence Pojer
    Insert
    PNGaseF
    Tags
    tufA-His-CBD-3C
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 41-354
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218193-plasmid-map-sequence-id-431798

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  38. pAL-hCul1-NTD

    Plasmid
    #29518
    Depositor
    Ning Zheng
    Insert
    CUL1 (CUL1 Human)
    Tags
    Thrombin Cleavage Site
    Expression
    Bacterial
    Mutation
    N-terminal 415 aa
    Available Since
    May 26, 2011
    Availability
    Academic Institutions and Nonprofits only

  39. pCC1R

    Plasmid
    #213497
    Purpose
    Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit
    Depositor
    Mark Isalan
    Inserts
    pcaU
    nahR
    rpaR (rpaR )
    phlF
    cinR
    vanR
    aiiA
    Use
    Synthetic Biology
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    213497-plasmid-map-sequence-id-435615

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  44. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  49. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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Showing: 6951 - 7000 of 14587 results